Näytä suppeat kuvailutiedot

dc.contributor.authorDinasquet, Julie
dc.contributor.authorTiirola, Marja
dc.contributor.authorAzam, Farooq
dc.date.accessioned2018-09-17T07:34:57Z
dc.date.available2018-09-17T07:34:57Z
dc.date.issued2018
dc.identifier.citationDinasquet, J., Tiirola, M., & Azam, F. (2018). Enrichment of Bacterioplankton Able to Utilize One-Carbon and Methylated Compounds in the Coastal Pacific Ocean. <i>Frontiers in Marine Science</i>, <i>5</i>, Article 307. <a href="https://doi.org/10.3389/fmars.2018.00307" target="_blank">https://doi.org/10.3389/fmars.2018.00307</a>
dc.identifier.otherCONVID_28223163
dc.identifier.otherTUTKAID_78592
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/59533
dc.description.abstractUnderstanding the temporal variations and succession of bacterial communities involved in the turnover of one-carbon and methylated compounds is necessary to better predict bacterial impacts on the marine carbon cycle and air-sea carbon fluxes. The ability of the local bacterioplankton community to exploit one-carbon and methylated compounds as main source of bioavailable carbon during a productive and less productive period was assessed through enrichment experiments. Surface seawater was amended with methanol and trimethylamine-N-oxide (TMAO), and bacterial abundance, production, oxygen consumption, as well as methanol turnover and growth rates of putative methylotrophs were followed. Bacterial community structure and functional diversity was examined through amplicon sequencing of 16S rRNA and methanol dehydrogenase (mxaF) marker genes. 2-fold increase in oxygen consumption and bacterial growth rates, and up to 4-fold higher methanol assimilation were observed in the amended seawater samples. Capacity to drawdown the substrates was similar between both experiments. In less productive conditions, methanol enriched obligate methylotrophs, especially Methylophaga spp., accounted for ∼70% of bacterial cells analyzed by fluorescence in situ hybridization and 16S rRNA gene sequencing, while TMAO enriched taxa belonged to Oceanospirillales and putative β- and γ-Proteobacterial methylotrophs. In the experiment performed during the more productive period, bacterial communities were structurally resistant, suggesting that facultative organisms may have dominated the observed methylotrophic activity. Moreover, enrichment of distinct methylotrophic taxa but similar activity rates observed in response to different substrate additions suggests a functional redundancy of substrate specific marine methylotrophic populations. Marine bacterioplankton cycling of one-carbon and methylated compounds appears to depend on the system productivity, and hence may have predictable temporal impacts on air-sea fluxes of volatile organic compounds.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherFrontiers Research Foundation
dc.relation.ispartofseriesFrontiers in Marine Science
dc.rightsCC BY 4.0
dc.rights© 2018 Dinasquet, Tiirola and Azam
dc.subject.otherbacterial community composition
dc.subject.othercoastal ecosys
dc.titleEnrichment of Bacterioplankton Able to Utilize One-Carbon and Methylated Compounds in the Coastal Pacific Ocean
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201809074049
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineYmpäristötiedefi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineEnvironmental Scienceen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2018-09-07T12:15:12Z
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2296-7745
dc.relation.numberinseries0
dc.relation.volume5
dc.type.versionpublishedVersion
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber615146
dc.relation.grantnumber615146
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/615146/EU//
dc.subject.ysoplankton
dc.subject.ysobakteerit
dc.subject.ysoekosysteemit (ekologia)
dc.subject.ysometanoli
dc.subject.ysorannikkoalueet
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p3053
jyx.subject.urihttp://www.yso.fi/onto/yso/p1749
jyx.subject.urihttp://www.yso.fi/onto/yso/p4997
jyx.subject.urihttp://www.yso.fi/onto/yso/p16842
jyx.subject.urihttp://www.yso.fi/onto/yso/p26383
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3389/fmars.2018.00307
dc.relation.funderEuroopan komissiofi
dc.relation.funderEuropean Commissionen
jyx.fundingprogramEU:n 7. puiteohjelma (FP7)fi
jyx.fundingprogramFP7 (EU's 7th Framework Programme)en
jyx.fundinginformationThis work was supported by a Marie Curie Actions-International Outgoing Fellowship (PIOF-GA-2013-629378 to JD), the European Research Council (Consolidator Grant 615146 to MT) and the Gordon and Betty Moore Foundation (Grant GBMF4827 to FA).
dc.type.okmA1


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