Enrichment of Bacterioplankton Able to Utilize One-Carbon and Methylated Compounds in the Coastal Pacific Ocean
Dinasquet, J., Tiirola, M., & Azam, F. (2018). Enrichment of Bacterioplankton Able to Utilize One-Carbon and Methylated Compounds in the Coastal Pacific Ocean. Frontiers in Marine Science, 5, Article 307. https://doi.org/10.3389/fmars.2018.00307
Published inFrontiers in Marine Science
Understanding the temporal variations and succession of bacterial communities involved in the turnover of one-carbon and methylated compounds is necessary to better predict bacterial impacts on the marine carbon cycle and air-sea carbon fluxes. The ability of the local bacterioplankton community to exploit one-carbon and methylated compounds as main source of bioavailable carbon during a productive and less productive period was assessed through enrichment experiments. Surface seawater was amended with methanol and trimethylamine-N-oxide (TMAO), and bacterial abundance, production, oxygen consumption, as well as methanol turnover and growth rates of putative methylotrophs were followed. Bacterial community structure and functional diversity was examined through amplicon sequencing of 16S rRNA and methanol dehydrogenase (mxaF) marker genes. 2-fold increase in oxygen consumption and bacterial growth rates, and up to 4-fold higher methanol assimilation were observed in the amended seawater samples. Capacity to drawdown the substrates was similar between both experiments. In less productive conditions, methanol enriched obligate methylotrophs, especially Methylophaga spp., accounted for ∼70% of bacterial cells analyzed by fluorescence in situ hybridization and 16S rRNA gene sequencing, while TMAO enriched taxa belonged to Oceanospirillales and putative β- and γ-Proteobacterial methylotrophs. In the experiment performed during the more productive period, bacterial communities were structurally resistant, suggesting that facultative organisms may have dominated the observed methylotrophic activity. Moreover, enrichment of distinct methylotrophic taxa but similar activity rates observed in response to different substrate additions suggests a functional redundancy of substrate specific marine methylotrophic populations. Marine bacterioplankton cycling of one-carbon and methylated compounds appears to depend on the system productivity, and hence may have predictable temporal impacts on air-sea fluxes of volatile organic compounds. ...
PublisherFrontiers Research Foundation
Publication in research information system
MetadataShow full item record
Related funder(s)European Commission
Funding program(s)FP7 (EU's 7th Framework Programme)
The content of the publication reflects only the author’s view. The funder is not responsible for any use that may be made of the information it contains.
Additional information about fundingThis work was supported by a Marie Curie Actions-International Outgoing Fellowship (PIOF-GA-2013-629378 to JD), the European Research Council (Consolidator Grant 615146 to MT) and the Gordon and Betty Moore Foundation (Grant GBMF4827 to FA).
Showing items with similar title or keywords.
Roiha, Toni (University of Jyväskylä, 2015)
Effects of fish community composition on ontogenetic niche shifts of Arctic charr (Salvelinus alpinus) and brown trout (Salmo trutta) in subarctic lakes Kangosjärvi, Henna (2021)Ravintoverkkotutkimukset yhdistävät biodiversiteetin sekä ekosysteemien toiminnan ja tarjoavat täten kokonaisvaltaisemman näkökulman suojelubiologisiin tutkimuksiin. Ravintoverkkotutkimuksissa on tärkeä huomioida generalistien ...
Allochthonous carbon is a major regulator to bacterial growth and community composition in subarctic freshwaters Roiha, Toni; Peura, Sari; Cusson, Mathieu; Rautio, Milla (Nature Publishing Group, 2016)In the subarctic region, climate warming and permafrost thaw are leading to emergence of ponds and to an increase in mobility of catchment carbon. As carbon of terrestrial origin is increasing in subarctic freshwaters ...
Poosakkannu, Anbu; Nissinen, Riitta; Kytöviita, Minna-Maarit (Springer, 2017)The effects of arbuscular mycorrhizal (AM) fungi on plant-associated microbes are poorly known. We tested the hypothesis that colonization by an AM fungus affects microbial species richness and microbial community composition ...
Ecosystem responses to increased organic carbon concentration : comparing results based on long-term monitoring and whole-lake experimentation Kankaala, Paula; Arvola, Lauri; Hiltunen, Minna; Huotari, Jussi; Jones, Roger I.; Nykänen, Hannu; Ojala, Anne; Olin, Mikko; Peltomaa, Elina; Peura, Sari; Rask, Martti; Tulonen, Tiina; Vesala, Sami (Taylor & Francis, 2019)Recent increases in terrestrial dissolved organic carbon (DOC) concentrations in northern inland waters have many ecological consequences. We examined available data on carbon cycles and food webs of 2 boreal headwater ...