dc.contributor.author | Hopkins, David | |
dc.contributor.author | Envall, Tapio | |
dc.contributor.author | Poikela, Noora | |
dc.contributor.author | Pentikäinen, Olli | |
dc.contributor.author | Kankare, Maaria | |
dc.date.accessioned | 2018-05-25T10:57:35Z | |
dc.date.available | 2018-05-25T10:57:35Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Hopkins, D., Envall, T., Poikela, N., Pentikäinen, O., & Kankare, M. (2018). Effects of cold acclimation and dsRNA injections on Gs1l gene splicing in Drosophila montana. <i>Scientific Reports</i>, <i>8</i>, Article 7577. <a href="https://doi.org/10.1038/s41598-018-25872-0" target="_blank">https://doi.org/10.1038/s41598-018-25872-0</a> | |
dc.identifier.other | CONVID_28057690 | |
dc.identifier.other | TUTKAID_77667 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/58124 | |
dc.description.abstract | Alternative splicing, in which one gene produce multiple transcripts, may infuence how adaptive genes
respond to specifc environments. A newly produced transcriptome of Drosophila montana shows the
Gs1-like (Gs1l) gene to express multiple splice variants and to be down regulated in cold acclimated
fies with increased cold tolerance. Gs1l’s efect on cold tolerance was further tested by injecting cold
acclimated and non-acclimated fies from two distantly located northern and southern fy populations
with double stranded RNA (dsRNA) targeting Gs1l. While both populations had similar cold acclimation
responses, dsRNA injections only efected the northern population. The nature of splicing expression
was then investigated in the northern population by confrming which Gs1l variants are present, by
comparing the expression of diferent gene regions and by predicting the protein structures of splices
using homology modelling. We fnd diferent splices of Gs1l not only appear to have independent
impacts on cold acclimation but also elicit diferent efects in populations originating from two very
diferent environments. Also, at the protein level, Gs1l appears homologous to the human HDHD1A
protein and some splices might produce functionally diferent proteins though this needs to be verifed
in future studies by measuring the particular protein levels. Taken together, Gs1l appears to be an
interesting new candidate to test how splicing infuences adaptations. | fi |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | |
dc.publisher | Nature Publishing Group | |
dc.relation.ispartofseries | Scientific Reports | |
dc.rights | CC BY 4.0 | |
dc.subject.other | kylmänkestävyys | fi |
dc.subject.other | akklimatisaatio | fi |
dc.subject.other | RNA | fi |
dc.subject.other | geenit | fi |
dc.subject.other | mahlakärpäset | fi |
dc.subject.other | cold resistance | fi |
dc.subject.other | acclimation | fi |
dc.subject.other | genes | fi |
dc.subject.other | Drosophilidae | fi |
dc.title | Effects of cold acclimation and dsRNA injections on Gs1l gene splicing in Drosophila montana | |
dc.type | article | |
dc.identifier.urn | URN:NBN:fi:jyu-201805212697 | |
dc.contributor.laitos | Bio- ja ympäristötieteiden laitos | fi |
dc.contributor.laitos | Department of Biological and Environmental Science | en |
dc.contributor.oppiaine | Ekologia ja evoluutiobiologia | fi |
dc.contributor.oppiaine | Ecology and Evolutionary Biology | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.date.updated | 2018-05-21T09:15:10Z | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.relation.issn | 2045-2322 | |
dc.relation.numberinseries | 0 | |
dc.relation.volume | 8 | |
dc.type.version | publishedVersion | |
dc.rights.copyright | © the Authors, 2018. | |
dc.rights.accesslevel | openAccess | fi |
dc.relation.grantnumber | 268214 | |
dc.subject.yso | kylmänkestävyys | |
dc.subject.yso | mahlakärpäset | |
dc.subject.yso | akklimatisaatio | |
dc.subject.yso | RNA | |
dc.subject.yso | geenit | |
dc.format.content | fulltext | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p3062 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p12159 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p25030 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p7689 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p147 | |
dc.rights.url | https://creativecommons.org/licenses/by/4.0/ | |
dc.relation.doi | 10.1038/s41598-018-25872-0 | |
dc.relation.funder | Suomen Akatemia | fi |
dc.relation.funder | Research Council of Finland | en |
jyx.fundingprogram | Akatemiatutkija, SA | fi |
jyx.fundingprogram | Academy Research Fellow, AoF | en |
jyx.fundinginformation | We thank David Dolezel and his lab for the guidance in the dsRNA injections in D. montana, Kalevi Trontti for the input of bringing the dsRNA system to our laboratory and the original primer design of the first Gs1l transcripts and Ville Hoikkala for the help with the first test dsRNA injections. Darren J. Parker is thanked for the input in the transcriptome analyses. We also like to thank Sara Calhim and Sebastiano De Bona for their statistical advice. The project was supported by the grants from Academy of Finland to MK (projects 268214 and 272927). | |
dc.type.okm | A1 | |