Photosensitive Alternative Splicing of the Circadian Clock Gene timeless Is Population Specific in a Cold-Adapted Fly, Drosophila montana
Tapanainen, R., Parker, D., & Kankare, M. (2018). Photosensitive Alternative Splicing of the Circadian Clock Gene timeless Is Population Specific in a Cold-Adapted Fly, Drosophila montana. G3: Genes, Genomes, Genetics, 8(4), 1291-1297. https://doi.org/10.1534/g3.118.200050
Published inG3: Genes, Genomes, Genetics
© 2018 Tapanainen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License.
To function properly, organisms must adjust their physiology, behavior and metabolism in response to a suite of varying environmental conditions. One of the central regulators of these changes is organisms’ internal circadian clock, and recent evidence has suggested that the clock genes are also important in the regulation of seasonal adjustments. In particular, thermosensitive splicing of the core clock gene timeless in a cosmopolitan fly, Drosophila melanogaster, has implicated this gene to be involved in thermal adaptation. To further investigate this link we examined the splicing of timeless in a northern malt fly species, Drosophila montana, which can withstand much colder climatic conditions than its southern relative. We studied northern and southern populations from two different continents (North America and Europe) to find out whether and how the splicing of this gene varies in response to different temperatures and day lengths. Interestingly, we found that the expression of timeless splice variants was sensitive to differences in light conditions, and while the flies of all study populations showed a change in the usage of splice variants in constant light compared to LD 22:2, the direction of the shift varied between populations. Overall, our findings suggest that the splicing of timeless in northern Drosophila montana flies is photosensitive, rather than thermosensitive and highlights the value of studying multiple species and populations in order to gain perspective on the generality of gene function changes in different kinds of environmental conditions. ...
PublisherGenetics Society of America
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Related funder(s)Academy of Finland
Funding program(s)Research post as Academy Research Fellow, AoF
Additional information about fundingWe thank Kalevi Trontti for the guidance in the molecular cloning, Tapio Envall for the help with the expression analysis calculations and Anneli Hoikkala for critical reading of the manuscript, valuable comments and original preparing of the Supplementary Table S8 in File S1. The project was supported by the grants from Academy of Finland to MK (projects 268214 and 272927).
Except where otherwise noted, this item's license is described as © 2018 Tapanainen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License.
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