High-affinity and selective detection of pyrophosphate in water by a resorcinarene salt receptor
Beyeh, N. K., Díez, I., Taimoory, S. M., Meister, D., Feig, A. I., Trant, J. F., Ras, R. H. A., & Rissanen, K. (2018). High-affinity and selective detection of pyrophosphate in water by a resorcinarene salt receptor. Chemical Science, 9(5), 1358-1367. https://doi.org/10.1039/C7SC05167K
Published inChemical Science
© the Authors, The Royal Society of Chemistry 2018. This is an open access article distributed under the terms of the Creative Commons License.
Pyrophosphate (PPi) is a byproduct of DNA and RNA synthesis, and abnormal levels are indicative of disease. We report the high-affinity binding of PPi in water by N-alkyl ammonium resorcinarene chloride receptors. Experimental analysis using 1H and 31P NMR, isothermal titration calorimetry, mass spectrometry, and UV-vis spectroscopy all support exceptional selectivity of these systems for PPi in water. The measured affinity of K1 = 1.60 × 107 M−1 for PPi is three orders of magnitude larger than that observed for binding to another phosphate, ATP. This exceptional anion-binding affinity in water is explored through a detailed density functional theory computational study. These systems provide a promising avenue for the development of future innovative medical diagnostic tools.