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dc.contributor.authorKoskinen, Satu
dc.contributor.authorKyröläinen, Heikki
dc.contributor.authorFlink, Riina
dc.contributor.authorSelänne, Harri
dc.contributor.authorGagnon, Sheila S.
dc.contributor.authorAhtiainen, Juha
dc.contributor.authorNindl, Bradley C.
dc.contributor.authorLehti, Maarit
dc.date.accessioned2018-01-30T06:51:38Z
dc.date.available2018-12-02T22:35:58Z
dc.date.issued2017
dc.identifier.citationKoskinen, S., Kyröläinen, H., Flink, R., Selänne, H., Gagnon, S. S., Ahtiainen, J., Nindl, B. C., & Lehti, M. (2017). Human skeletal muscle type 1 fibre distribution and response of stress-sensing proteins along the titin molecule after submaximal exhaustive exercise. <i>Histochemistry and Cell Biology</i>, <i>148</i>(5), 545-555. <a href="https://doi.org/10.1007/s00418-017-1595-z" target="_blank">https://doi.org/10.1007/s00418-017-1595-z</a>
dc.identifier.otherCONVID_27121619
dc.identifier.otherTUTKAID_74457
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/56944
dc.description.abstractEarly responses of stress-sensing proteins, muscle LIM protein (MLP), ankyrin repeat proteins (Ankrd1/CARP and Ankrd2/Arpp) and muscle-specific RING finger proteins (MuRF1 and MuRF2), along the titin molecule were investigated in the present experiment after submaximal exhaustive exercise. Ten healthy men performed continuous drop jumping unilaterally on a sledge apparatus with a submaximal height until complete exhaustion. Five stress-sensing proteins were analysed by mRNA measurements from biopsies obtained immediately and 3 h after the exercise from exercised vastus lateralis muscle while control biopsies were obtained from non-exercised legs before the exercise. Decreased maximal jump height and increased serum creatine kinase activities as indirect markers for muscle damage and HSP27 immunostainings on muscle biopsies as a direct marker for muscle damage indicated that the current exercised protocol caused muscle damage. mRNA levels for four (MLP, Ankrd1/CARP, MuRF1 and MuRF2) out of the five studied stress sensors significantly (p < 0.05) increased 3 h after fatiguing exercise. The magnitude of MLP and Ankrd2 responses was related to the proportion of type 1 myofibres. Our data showed that the submaximal exhaustive exercise with subject’s own physical fitness level activates titin-based stretch-sensing proteins. These results suggest that both degenerative and regenerative pathways are activated in very early phase after the exercise or probably already during the exercise. Activation of these proteins represents an initial step forward adaptive remodelling of the exercised muscle and may also be involved in the initiation of myofibre repair.
dc.languageeng
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofseriesHistochemistry and Cell Biology
dc.subject.otherhuman skeletal muscle
dc.subject.otherexercise induced muscle damage
dc.subject.othertitin
dc.subject.otherstress-sensing proteins
dc.titleHuman skeletal muscle type 1 fibre distribution and response of stress-sensing proteins along the titin molecule after submaximal exhaustive exercise
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201801291360
dc.contributor.laitosLiikuntatieteellinen tiedekuntafi
dc.contributor.laitosFaculty of Sport and Health Sciencesen
dc.contributor.oppiaineValmennus- ja testausoppifi
dc.contributor.oppiaineScience of Sport Coaching and Fitness Testingen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2018-01-29T13:15:09Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.format.pagerange545-555
dc.relation.issn0948-6143
dc.relation.numberinseries5
dc.relation.volume148
dc.type.versionacceptedVersion
dc.rights.copyright© Springer-Verlag GmbH Germany, 2017. This is a final draft version of an article whose final and definitive form has been published by Springer-Verlag GmbH Germany. Published in this repository with the kind permission of the publisher.
dc.rights.accesslevelopenAccessfi
dc.subject.ysolihakset
dc.subject.ysotuki- ja liikuntaelimet
dc.subject.ysourheiluvammat
jyx.subject.urihttp://www.yso.fi/onto/yso/p2784
jyx.subject.urihttp://www.yso.fi/onto/yso/p2785
jyx.subject.urihttp://www.yso.fi/onto/yso/p12766
dc.relation.doi10.1007/s00418-017-1595-z


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