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dc.contributor.authorSaarinen, Niila V. V.
dc.contributor.authorLaiho, Jutta E.
dc.contributor.authorRichardson, Sarah J.
dc.contributor.authorZeissler, Marie
dc.contributor.authorStone, Virginia M.
dc.contributor.authorMarjomäki, Varpu
dc.contributor.authorKantoluoto, Tino
dc.contributor.authorHorwitz, Marc S.
dc.contributor.authorSioofy-Khojine, Amirbabak
dc.contributor.authorHonkimaa, Anni
dc.contributor.authorHankaniemi, Minna M.
dc.contributor.authorFlodström-Tullberg, Malin
dc.contributor.authorHyöty, Heikki
dc.contributor.authorHytönen, Vesa P.
dc.contributor.authorLaitinen, Olli H.
dc.date.accessioned2018-01-18T10:51:22Z
dc.date.available2018-01-18T10:51:22Z
dc.date.issued2018
dc.identifier.citationSaarinen, N. V. V., Laiho, J. E., Richardson, S. J., Zeissler, M., Stone, V. M., Marjomäki, V., . . . Laitinen, O. H. (2018). A novel rat CVB1-VP1 monoclonal antibody 3A6 detects a broad range of enteroviruses. <em>Scientific Reports</em>, 8, 33. <a href="https://doi.org/10.1038/s41598-017-18495-4">doi:10.1038/s41598-017-18495-4</a>
dc.identifier.otherTUTKAID_76509
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/56789
dc.description.abstractEnteroviruses (EVs) are common RNA viruses that cause diseases ranging from rash to paralytic poliomyelitis. For example, EV-A and EV-C viruses cause hand-foot and mouth disease and EV-B viruses cause encephalitis and myocarditis, which can result in severe morbidity and mortality. While new vaccines and treatments for EVs are under development, methods for studying and diagnosing EV infections are still limited and therefore new diagnostic tools are required. Our aim was to produce and characterize new antibodies that work in multiple applications and detect EVs in tissues and in vitro. Rats were immunized with Coxsackievirus B1 capsid protein VP1 and hybridomas were produced. Hybridoma clones were selected based on their reactivity in different immunoassays. The most promising clone, 3A6, was characterized and it performed well in multiple techniques including ELISA, immunoelectron microscopy, immunocyto- and histochemistry and in Western blotting, detecting EVs in infected cells and tissues. It recognized several EV-Bs and also the EV-C representative Poliovirus 3, making it a broad-spectrum EV specific antibody. The 3A6 rat monoclonal antibody can help to overcome some of the challenges faced with commonly used EV antibodies: it enables simultaneous use of mouse-derived antibodies in double staining and it is useful in murine models.
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofseriesScientific Reports
dc.subject.othervasta-aineet
dc.subject.otherenterovirukset
dc.subject.otherantibodies
dc.subject.otherenteroviruses
dc.titleA novel rat CVB1-VP1 monoclonal antibody 3A6 detects a broad range of enteroviruses
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201801151192
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosThe Department of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologia
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2018-01-15T13:16:11Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.relation.issn2045-2322
dc.relation.volume8
dc.type.versionpublishedVersion
dc.rights.copyright© the Authors, 2017. This is an open access article distributed under the terms of the Creative Commons License.
dc.rights.accesslevelopenAccessfi
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1038/s41598-017-18495-4


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© the Authors, 2017. This is an open access article distributed under the terms of the Creative Commons License.
Except where otherwise noted, this item's license is described as © the Authors, 2017. This is an open access article distributed under the terms of the Creative Commons License.