Gliding motility and expression of motility-related genes in spreading and nonspreading colonies of Flavobacterium columnare
Lataukset:
Penttinen, R., Hoikkala, V., & Sundberg, L.-R. (2018). Gliding Motility and Expression of Motility-Related Genes in Spreading and Non-spreading Colonies of Flavobacterium columnare. Frontiers in Microbiology, 9, 525. doi:10.3389/fmicb.2018.00525
Julkaistu sarjassa
Frontiers in MicrobiologyPäivämäärä
2018Tekijänoikeudet
© 2018 Penttinen, Hoikkala and Sundberg. This is an open access article distributed under the terms of the Creative Commons License.
Gliding motility machinery enables moving on surfaces in many species among
Bacteroidetes, resulting in bacterial colonies with spreading appearance. The adhesins required for gliding are secreted through a gliding motility –associated protein secretion
system known as the type IX secretion system (T9SS). The fish pathogen Flavobacterium
columnare produces spreading (Rhizoid, Rz; Soft, S) and nonspreading (Rough, R) colony
types, of which only the spreading Rz type is virulent. In this study, we explored the
spreading behaviour of these colony types by microscopic imaging and measured the
expression of genes associated with gliding motility and T9SS (gldG, gldH, gldL, sprA, sprB, sprE, sprF, sprT and porV) under high and low resource levels. The spreading colony types responded to low resource level by increased colony size. The nonspreading colony type as
well as the cells subjected to high nutrient level expressed only moderate cell movements.
Yet, low nutrient level provoked more active gliding motility by individual cells and
increased biofilm spreading by cooperative gliding. The gene expression survey
demonstrated an increased expression level of sprA and sprF under low nutrient conditions.
Surprisingly, the expression of gliding motility genes was not consistently associated with
more active spreading behaviour. Our study demonstrates that environmental nutrient level is
an important regulator of gliding motility and also the expression of some of the associated
genes. Furthermore, our results may help to understand the connections between nutrient
concentration, gliding motility and virulence of F. columnare.
...
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Frontiers Research FoundationISSN Hae Julkaisufoorumista
1664-302XAsiasanat
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