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dc.contributor.authorAshrafi, Roghaieh
dc.contributor.authorBruneaux, Matthieu
dc.contributor.authorSundberg, Lotta-Riina
dc.contributor.authorPulkkinen, Katja
dc.contributor.authorKetola, Tarmo
dc.date.accessioned2017-04-26T10:34:34Z
dc.date.available2017-04-26T10:34:34Z
dc.date.issued2017
dc.identifier.citationAshrafi, R., Bruneaux, M., Sundberg, L.-R., Pulkkinen, K., & Ketola, T. (2017). Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium. <i>Scientific Reports</i>, <i>7</i>, Article 980. <a href="https://doi.org/10.1038/s41598-017-01074-y" target="_blank">https://doi.org/10.1038/s41598-017-01074-y</a>
dc.identifier.otherCONVID_26972349
dc.identifier.otherTUTKAID_73612
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/53693
dc.description.abstractStudies on species’ responses to climate change have focused largely on the direct effect of abiotic factors and in particular temperature, neglecting the effects of biotic interactions in determining the outcome of climate change projections. Many microbes rely on strong interference competition; hence the fitness of many pathogenic bacteria could be a function of both their growth properties and intraspecific competition. However, due to technical challenges in distinguishing and tracking individual strains, experimental evidence on intraspecific competition has been limited so far. Here, we developed a robust application of the high-resolution melting (HRM) assay to study head-to-head competition between mixed genotype co-cultures of a waterborne bacterial pathogen of fish, Flavobacterium columnare, at two different temperatures. We found that competition outcome in liquid cultures seemed to be well predicted by growth yield of isolated strains, but was mostly inconsistent with interference competition results measured in inhibition tests on solid agar, especially as no growth inhibition between strain pairs was detected at the higher temperature. These results suggest that, for a given temperature, the factors driving competition outcome differ between liquid and solid environments.en
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofseriesScientific Reports
dc.subject.otherpathogenic bacteria
dc.subject.otherintraspecific competition
dc.subject.otherhigh-resolution melting (HRM) assay
dc.titleApplication of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201704252083
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineEkologia ja evoluutiobiologiafi
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineAkvaattiset tieteetfi
dc.contributor.oppiaineBiologisten vuorovaikutusten huippututkimusyksikköfi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineEcology and Evolutionary Biologyen
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineAquatic Sciencesen
dc.contributor.oppiaineCentre of Excellence in Biological Interactions Researchen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2017-04-25T15:15:04Z
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2045-2322
dc.relation.numberinseries0
dc.relation.volume7
dc.type.versionpublishedVersion
dc.rights.copyright© the Authors, 2017. This article is licensed under a Creative Commons Attribution 4.0 International License.
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber278751
dc.subject.ysolämpötila
jyx.subject.urihttp://www.yso.fi/onto/yso/p2100
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.datasethttps://jyx.jyu.fi/dspace/handle/123456789/53541
dc.relation.doi10.1038/s41598-017-01074-y
dc.relation.funderSuomen Akatemiafi
dc.relation.funderResearch Council of Finlanden
jyx.fundingprogramAkatemiatutkija, SAfi
jyx.fundingprogramAcademy Research Fellow, AoFen
jyx.fundinginformationThis work was supported by KONE foundation (Roghaieh Ashrafi via project “Constraints of evolutionary adaptation to climate change” to Tarmo Ketola), OLVI foundation (Roghaieh Ashrafi #201620393), the Jane and Aatos Erkko Foundation (Lotta-Riina Sundberg), Finnish Cultural Foundation (Katja Pulkkinen) and Academy of Finland (Lotta-Riina Sundberg #272995, Tarmo Ketola #278751, Jouni Taskinen #260704 to Katja Pulkkinen) and Centre of Excellence in Biological Interactions (#252411, Prof. Johanna Mappes) to Roghaieh Ashrafi, Lotta-Riina Sundberg, and Tarmo Ketola.
dc.type.okmA1


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© the Authors, 2017. This article is licensed under a Creative Commons Attribution 4.0 International License.
Except where otherwise noted, this item's license is described as © the Authors, 2017. This article is licensed under a Creative Commons Attribution 4.0 International License.