Research data of an article: "Application of high resolution melting assay (HMR) to study temperature dependent infraspecific competition in an pathogenic bacterium."
© University of Jyväskylä, 2017. This research data is available with the permission of the authors. Please contact the corresponding author.
Studies on species’ responses to climate change have focused largely on the direct effect of abiotic factors and in particular temperature, neglecting the effects of biotic interactions in determining the outcome of climate change projections. Many microbes rely on strong interference competition; hence the fitness of many pathogenic bacteria could be a function of both their growth properties and intraspecific competition. However, due to technical challenges in distinguishing and tracking individual strains, experimental evidence on intraspecific competition has been limited so far. Here, we developed a robust application of the high-resolution melting (HRM) assay to study head-to-head competition between mixed genotype co-cultures of a waterborne bacterial pathogen of fish, Flavobacterium columnare, at two different temperatures. We found that competition outcome in liquid cultures seemed to be well predicted by growth yield of isolated strains, but was mostly inconsistent with interference competition results measured in inhibition tests on solid agar, especially as no growth inhibition between strain pairs was detected at the higher temperature. These results suggest that, for a given temperature, the factors driving competition outcome differ between liquid and solid environments. ...
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Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium Ashrafi, Roghaieh; Bruneaux, Matthieu; Sundberg, Lotta-Riina; Pulkkinen, Katja; Ketola, Tarmo (Nature Publishing Group, 2017)Studies on species’ responses to climate change have focused largely on the direct effect of abiotic factors and in particular temperature, neglecting the effects of biotic interactions in determining the outcome of climate ...
Kinnula, Hanna; Mappes, Johanna; Sundberg, Lotta-Riina (BioMed Central, 2017)Background: In nature, organisms are commonly coinfected by two or more parasite strains, which has been shown to influence disease virulence. Yet, the effects of coinfections of environmental opportunistic pathogens ...
Laanto, Elina (University of Jyväskylä, 2014)
Effect of resource availability on evolution of virulence and competition in an environmentally transmitted pathogen Pulkkinen, Katja; Pekkala, Nina; Ashrafi, Roghaieh; Hämäläinen, Dorrit M.; Nkembeng, Aloysius N.; Lipponen, Anssi; Hiltunen, Teppo; Valkonen, Janne; Taskinen, Jouni (Federation of European Microbiological Societies; Oxford University Press, 2018)Understanding ecological and epidemiological factors driving pathogen evolution in contemporary time scales is a major challenge in modern health management. Pathogens that replicate outside the hosts are subject to selection ...
Exploring evolutionary responses to increasing temperature in an environmental opportunistic pathogen Ashrafi, Roghaieh (University of Jyväskylä, 2017)