Research data of an article: "Application of high resolution melting assay (HMR) to study temperature dependent infraspecific competition in an pathogenic bacterium."
© University of Jyväskylä, 2017. This research data is available with the permission of the authors. Please contact the corresponding author.
Studies on species’ responses to climate change have focused largely on the direct effect of abiotic factors and in particular temperature, neglecting the effects of biotic interactions in determining the outcome of climate change projections. Many microbes rely on strong interference competition; hence the fitness of many pathogenic bacteria could be a function of both their growth properties and intraspecific competition. However, due to technical challenges in distinguishing and tracking individual strains, experimental evidence on intraspecific competition has been limited so far. Here, we developed a robust application of the high-resolution melting (HRM) assay to study head-to-head competition between mixed genotype co-cultures of a waterborne bacterial pathogen of fish, Flavobacterium columnare, at two different temperatures. We found that competition outcome in liquid cultures seemed to be well predicted by growth yield of isolated strains, but was mostly inconsistent with interference competition results measured in inhibition tests on solid agar, especially as no growth inhibition between strain pairs was detected at the higher temperature. These results suggest that, for a given temperature, the factors driving competition outcome differ between liquid and solid environments. ...
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Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium Ashrafi, Roghaieh; Bruneaux, Matthieu; Sundberg, Lotta-Riina; Pulkkinen, Katja; Ketola, Tarmo (Nature Publishing Group, 2017)Studies on species’ responses to climate change have focused largely on the direct effect of abiotic factors and in particular temperature, neglecting the effects of biotic interactions in determining the outcome of climate ...
Does the freshwater mussel Anodonta anatina remove the fish pathogen Flavobacterium columnare from water? Hajisafarali, Mahsa; Aaltonen, Sari; Pulkkinen, Katja; Taskinen, Jouni (Springer, 2022)Global decline of freshwater mussels (Unionoida) is threatening biodiversity and the essential ecosystem services that mussels provide. As filter-feeding organisms, freshwater mussels remove phytoplankton and suspended ...
Characterizing the bacterial fish pathogen Flavobacterium columnare, and some factors affecting its pathogenicity Kunttu, Heidi (University of Jyväskylä, 2010)Flavobacterium columnare is a bacterial pathogen of fresh water fish causing lethal columnaris disease worldwide. At Finnish fish farms, columnaris disease has occurred since the early 1980s, and is a serious problem in ...
Comparing the different morphotypes of a fish pathogen - implications for key virulence factors in Flavobacterium columnare Laanto, Elina; Penttinen, Reetta; Bamford, Jaana; Sundberg, Lotta-Riina (BioMed Central Ltd., 2014)Background: Flavobacterium columnare (Bacteroidetes) is the causative agent of columnaris disease in farmed freshwater fish around the world. The bacterium forms three colony morphotypes (Rhizoid, Rough and Soft), ...
Effect of nutrients on competition between a virulent and a less virulent strain of the fish pathogen Flavobacterium columnare Hämäläinen, Dorrit (2016)Patogeenit ovat haitallisia eliöitä, jotka tarvitsevat isäntää saadakseen tarvittavia ravinteita. Patogeenin haitallisuutta kutsutaan virulenssiksi, ja se on tärkeä osuus patogeeni-isäntä–suhteessa. Virulenssia säädellään ...