Fine-tuning the extent and dynamics of binding cleft opening as a potential general regulatory mechanism in parvulin-type peptidyl prolyl isomerases
Czajlik, A., Kovács, B., Permi, P., & Gáspári, Z. (2017). Fine-tuning the extent and dynamics of binding cleft opening as a potential general regulatory mechanism in parvulin-type peptidyl prolyl isomerases. Scientific Reports, 7, Article 44504. https://doi.org/10.1038/srep44504
Julkaistu sarjassa
Scientific ReportsPäivämäärä
2017Oppiaine
Solu- ja molekyylibiologiaOrgaaninen kemiaNanoscience CenterCell and Molecular BiologyOrganic ChemistryNanoscience CenterTekijänoikeudet
© the Authors, 2017. This is an open access article distributed under under a Creative Commons Attribution 4.0 International License.
Parvulins or rotamases form a distinct group within peptidyl prolyl cis-trans isomerases. Their exact
mode of action as well as the role of conserved residues in the family are still not unambiguously
resolved. Using backbone S2 order parameters and NOEs as restraints, we have generated dynamic
structural ensembles of three distinct parvulins, SaPrsA, TbPin1 and CsPinA. The resulting ensembles
are in good agreement with the experimental data but reveal important differences between the three
enzymes. The largest difference can be attributed to the extent of the opening of the substrate binding
cleft, along which motional mode the three molecules occupy distinct regions. Comparison with a
wide range of other available parvulin structures highlights structural divergence along the bottom of
the binding cleft acting as a hinge during the opening-closing motion. In the prototype WW-domain
containing parvulin, Pin1, this region is also important in forming contacts with the WW domain known
to modulate enzymatic activity of the catalytic domain. We hypothesize that modulation of the extent
and dynamics of the identified ‘breathing motion’ might be one of the factors responsible for functional
differences in the distinct parvulin subfamilies.
...
Julkaisija
Nature Publishing GroupISSN Hae Julkaisufoorumista
2045-2322Asiasanat
Julkaisu tutkimustietojärjestelmässä
https://converis.jyu.fi/converis/portal/detail/Publication/26920944
Metadata
Näytä kaikki kuvailutiedotKokoelmat
Lisenssi
Ellei muuten mainita, aineiston lisenssi on © the Authors, 2017. This is an open access article distributed under under a Creative Commons Attribution 4.0 International License.
Samankaltainen aineisto
Näytetään aineistoja, joilla on samankaltainen nimeke tai asiasanat.
-
Phosphorylated immunoreceptor tyrosine-based activation motifs and integrin cytoplasmic domains activate spleen tyrosine kinase via distinct mechanisms
Antenucci, Lina; Hytönen, Vesa P.; Ylänne, Jari (American Society for Biochemistry and Molecular Biology, 2018)Spleen tyrosine kinase (Syk) is involved in cellular adhesion and also in the activation and development of hematopoietic cells. Syk activation induced by genomic rearrangement has been linked to certain T-cell lymphomas, ... -
Role of serine synthesis pathway parallel to glycolysis in C2C12 muscle cell proliferation and myotube hypertrophy potential : effect of phosphoglycerate dehydrogenase inhibition
Kolari, Kalle (2023)Pro-gradu tutkimuksen tarkoituksena on tuoda lisätietoa glykolyysin rinnakkaisen aineenvaihduntareitin, seriinisynteesin, roolista C2C12 lihassolujen jakaantumis- hypertrofia potentiaaliin. Potentiaalin muutoksia tarkastellaan ... -
Common and separable behavioral and neural mechanisms underlie the generalization of fear and disgust
Wang, Jinxia; Sun, Xiaoying; Becker, Benjamin; Lei, Yi (Elsevier BV, 2022)Generalization represents the transfer of a conditioned responses to stimuli that resemble the conditioned stimulus (CS). Previous studies on generalization of defensive avoidance responses have primarily focused on fear ... -
Influence of Perceptual and Conceptual Information on Fear Generalization : A Behavioral and Event-Related Potential Study
Wang, Jinxia; E, Mei; Wu, Qi; Xie, Tao; Dou, Haoran; Lei, Yi (Springer, 2021)Learned fear can be generalized through both perceptual and conceptual information. This study investigated how perceptual and conceptual similarities influence this generalization process. Twenty-three healthy volunteers ... -
Proposed mechanism for increased reproductive potential of wild boars under hunting pressure
Shanas, Uri; Davidson, Achiad; Malkinson, Dan (Open Science Centre, University of Jyväskylä, 2018)Throughout Europe and Asia, populations of wild boars (Sus scrofa) demonstrate a steady increase in recent decades. This results in increased conflicts between wild boars and humans, intensifying economic costs like epidemics ...
Ellei toisin mainittu, julkisesti saatavilla olevia JYX-metatietoja (poislukien tiivistelmät) saa vapaasti uudelleenkäyttää CC0-lisenssillä.