Show simple item record

dc.contributor.authorHuttunen, Moona
dc.contributor.authorTurkki, Paula
dc.contributor.authorMäki, Anita
dc.contributor.authorPaavolainen, L.
dc.contributor.authorRuusuvuori, P.
dc.contributor.authorMarjomäki, Varpu
dc.date.accessioned2017-02-14T12:08:42Z
dc.date.available2017-10-20T21:45:09Z
dc.date.issued2017
dc.identifier.citationHuttunen, M., Turkki, P., Mäki, A., Paavolainen, L., Ruusuvuori, P., & Marjomäki, V. (2017). Echovirus 1 internalization negatively regulates epidermal growth factor receptor downregulation. <i>Cellular Microbiology</i>, <i>19</i>(3), Article e12671. <a href="https://doi.org/10.1111/cmi.12671" target="_blank">https://doi.org/10.1111/cmi.12671</a>
dc.identifier.otherCONVID_26232255
dc.identifier.otherTUTKAID_71272
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/53034
dc.description.abstractWe have demonstrated previously that the human picornavirus Echovirus 1 (EV1) triggers an infectious internalization pathway that follows closely, but seems to stay separate, from the epidermal growth factor receptor (EGFR) pathway triggered by epidermal growth factor (EGF). Here, we confirmed by using live and confocal microscopy that EGFR and EV1 vesicles are following intimately each other but are distinct entities with different degradation kinetics. We show here that despite being sorted to different pathways and located in distinct endosomes, EV1 inhibits EGFR downregulation. Simultaneous treatment with EV1 and EGF led to an accumulation of EGFR in cytoplasmic endosomes, which was evident already 15 min p.i. and more pronounced after 2 hr p.i. EV1 treatment led to reduced downregulation, which was proven by increased total cellular amount of EGFR. Confocal microscopy studies revealed that EGFR accumulated in large endosomes, presumably macropinosomes, which were not positive for markers of the early, recycling, or late endosomes/lysosomes. Interestingly, EV1 did not have a similar blocking effect on bulk endosomal trafficking or transferrin recycling along the clathrin pathway suggesting that EV1 did not have a general effect on cellular trafficking pathways. Importantly, EGF treatment increased EV1 infection and increased cell viability during infection. Simultaneous EV1 and EGF treatment seemed to moderately enhance phosphorylation of protein kinase C α. Furthermore, similar phenotype of EGFR trafficking could be produced by phorbol 12‐myristate 13‐acetate treatment, further suggesting that activated protein kinase C α could be contributing to EGFR phenotype. These results altogether demonstrate that EV1 specifically affects EGFR trafficking, leading to EGFR downregulation, which is beneficial to EV1 infection.en
dc.languageeng
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing Ltd
dc.relation.ispartofseriesCellular Microbiology
dc.subject.otherenterovirus
dc.subject.otherepidermal growth factor receptor (EGFR)
dc.subject.othersignaling
dc.titleEchovirus 1 internalization negatively regulates epidermal growth factor receptor downregulation
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201702131421
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2017-02-13T13:15:05Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.relation.issn1462-5814
dc.relation.numberinseries3
dc.relation.volume19
dc.type.versionacceptedVersion
dc.rights.copyright© 2016 John Wiley & Sons. This is a final draft version of an article whose final and definitive form has been published by Wiley. Published in this repository with the kind permission of the publisher.
dc.rights.accesslevelopenAccessfi
dc.subject.ysoendosytoosi
dc.subject.ysopilaantuminen
jyx.subject.urihttp://www.yso.fi/onto/yso/p24291
jyx.subject.urihttp://www.yso.fi/onto/yso/p4660
dc.relation.doi10.1111/cmi.12671
jyx.fundinginformationAcademy of Finland. Grant Number: 257125


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record