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dc.contributor.authorEdlund, Petra
dc.contributor.authorTakala, Heikki
dc.contributor.authorClaesson, Elin
dc.contributor.authorHenry, Léocadie
dc.contributor.authorDods, Robert
dc.contributor.authorLehtivuori, Heli
dc.contributor.authorPanman, Matthijs
dc.contributor.authorPande, Kanupriya
dc.contributor.authorWhite, Thomas
dc.contributor.authorNakane, Takanori
dc.contributor.authorBerntsson, Oskar
dc.contributor.authorGustavsson, Emil
dc.contributor.authorBåth, Petra
dc.contributor.authorModi, Vaibhav
dc.contributor.authorRoy-Chowdhury, Shatabdi
dc.contributor.authorZook, James
dc.contributor.authorBerntsen, Peter
dc.contributor.authorPandey, Suraj
dc.contributor.authorPoudyal, Ishwor
dc.contributor.authorTenboer, Jason
dc.contributor.authorKupitz, Christopher
dc.contributor.authorBarty, Anton
dc.contributor.authorFromme, Petra
dc.contributor.authorKoralek, Jake D.
dc.contributor.authorTanaka, Tomoyuki
dc.contributor.authorSpence, John
dc.contributor.authorLiang, Mengning
dc.contributor.authorHunter, Mark S.
dc.contributor.authorBoutet, Sebastien
dc.contributor.authorNango, Eriko
dc.contributor.authorMoffat, Keith
dc.contributor.authorGroenhof, Gerrit
dc.contributor.authorIhalainen, Janne
dc.contributor.authorStojković, Emina A.
dc.contributor.authorSchmidt, Marius
dc.contributor.authorWestenhoff, Sebastian
dc.date.accessioned2016-10-26T11:36:31Z
dc.date.available2016-10-26T11:36:31Z
dc.date.issued2016
dc.identifier.citationEdlund, P., Takala, H., Claesson, E., Henry, L., Dods, R., Lehtivuori, H., . . . Westenhoff, S. (2016). The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography. <em>Scientific Reports</em>, 6, 35279. <a href="https://doi.org/10.1038/srep35279">doi:10.1038/srep35279</a>
dc.identifier.otherTUTKAID_71544
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/51689
dc.description.abstractPhytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophorebinding domains of the Deinococcus radiodurans phytochrome at 2.1Å resolution.The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser.We find overall good agreement compared to a crystal structure at 1.35Å resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX.
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofseriesScientific Reports
dc.subject.otherroom temperature
dc.subject.othercrystal structure
dc.subject.otherphytochromes
dc.subject.othercrystallography
dc.titleThe room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201610244425
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosFysiikan laitosfi
dc.contributor.laitosKemian laitosfi
dc.contributor.laitosThe Department of Biological and Environmental Scienceen
dc.contributor.laitosDepartment of Physicsen
dc.contributor.laitosDepartment of Chemistryen
dc.contributor.oppiaineSolu- ja molekyylibiologia
dc.contributor.oppiaineFysikaalinen kemia
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2016-10-24T12:15:11Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.format.pagerange35279
dc.relation.issn2045-2322
dc.relation.volume6
dc.type.versionpublishedVersion
dc.rights.copyright© the Authors, 2016. This is an open access article distributed under a Creative Commons Attribution 4.0 International License.
dc.rights.accesslevelopenAccessfi
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1038/srep35279


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© the Authors, 2016. This is an open access article distributed under a Creative Commons Attribution 4.0 International License.
Except where otherwise noted, this item's license is described as © the Authors, 2016. This is an open access article distributed under a Creative Commons Attribution 4.0 International License.