The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography
Edlund, P., Takala, H., Claesson, E., Henry, L., Dods, R., Lehtivuori, H., Panman, M., Pande, K., White, T., Nakane, T., Berntsson, O., Gustavsson, E., Båth, P., Modi, V., Roy-Chowdhury, S., Zook, J., Berntsen, P., Pandey, S., Poudyal, I., . . . Westenhoff, S. (2016). The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography. Scientific Reports, 6, 35279. https://doi.org/10.1038/srep35279
Published inScientific Reports
DisciplineSolu- ja molekyylibiologiaFysikaalinen kemiaNanoscience CenterCell and Molecular BiologyPhysical ChemistryNanoscience Center
© the Authors, 2016. This is an open access article distributed under a Creative Commons Attribution 4.0 International License.
Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophorebinding domains of the Deinococcus radiodurans phytochrome at 2.1Å resolution.The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser.We find overall good agreement compared to a crystal structure at 1.35Å resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX. ...
PublisherNature Publishing Group
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Except where otherwise noted, this item's license is described as © the Authors, 2016. This is an open access article distributed under a Creative Commons Attribution 4.0 International License.
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