dc.contributor.author | Edlund, Petra | |
dc.contributor.author | Takala, Heikki | |
dc.contributor.author | Claesson, Elin | |
dc.contributor.author | Henry, Léocadie | |
dc.contributor.author | Dods, Robert | |
dc.contributor.author | Lehtivuori, Heli | |
dc.contributor.author | Panman, Matthijs | |
dc.contributor.author | Pande, Kanupriya | |
dc.contributor.author | White, Thomas | |
dc.contributor.author | Nakane, Takanori | |
dc.contributor.author | Berntsson, Oskar | |
dc.contributor.author | Gustavsson, Emil | |
dc.contributor.author | Båth, Petra | |
dc.contributor.author | Modi, Vaibhav | |
dc.contributor.author | Roy-Chowdhury, Shatabdi | |
dc.contributor.author | Zook, James | |
dc.contributor.author | Berntsen, Peter | |
dc.contributor.author | Pandey, Suraj | |
dc.contributor.author | Poudyal, Ishwor | |
dc.contributor.author | Tenboer, Jason | |
dc.contributor.author | Kupitz, Christopher | |
dc.contributor.author | Barty, Anton | |
dc.contributor.author | Fromme, Petra | |
dc.contributor.author | Koralek, Jake D. | |
dc.contributor.author | Tanaka, Tomoyuki | |
dc.contributor.author | Spence, John | |
dc.contributor.author | Liang, Mengning | |
dc.contributor.author | Hunter, Mark S. | |
dc.contributor.author | Boutet, Sebastien | |
dc.contributor.author | Nango, Eriko | |
dc.contributor.author | Moffat, Keith | |
dc.contributor.author | Groenhof, Gerrit | |
dc.contributor.author | Ihalainen, Janne | |
dc.contributor.author | Stojković, Emina A. | |
dc.contributor.author | Schmidt, Marius | |
dc.contributor.author | Westenhoff, Sebastian | |
dc.date.accessioned | 2016-10-26T11:36:31Z | |
dc.date.available | 2016-10-26T11:36:31Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Edlund, P., Takala, H., Claesson, E., Henry, L., Dods, R., Lehtivuori, H., Panman, M., Pande, K., White, T., Nakane, T., Berntsson, O., Gustavsson, E., Båth, P., Modi, V., Roy-Chowdhury, S., Zook, J., Berntsen, P., Pandey, S., Poudyal, I., . . . Westenhoff, S. (2016). The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography. <i>Scientific Reports</i>, <i>6</i>, 35279. <a href="https://doi.org/10.1038/srep35279" target="_blank">https://doi.org/10.1038/srep35279</a> | |
dc.identifier.other | CONVID_26282980 | |
dc.identifier.other | TUTKAID_71544 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/51689 | |
dc.description.abstract | Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria.
A sequence of structural changes, which is not yet fully understood, leads to activation of an output
domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these
conformational changes. Here we report the room temperature crystal structure of the chromophorebinding
domains of the Deinococcus radiodurans phytochrome at 2.1Å resolution.The structure was
obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron
laser.We find overall good agreement compared to a crystal structure at 1.35Å resolution derived from
conventional crystallography at cryogenic temperatures, which we also report here. The thioether
linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but
is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the
phytochrome photocycle with time-resolved SFX. | |
dc.language.iso | eng | |
dc.publisher | Nature Publishing Group | |
dc.relation.ispartofseries | Scientific Reports | |
dc.subject.other | room temperature | |
dc.subject.other | crystal structure | |
dc.subject.other | phytochromes | |
dc.title | The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography | |
dc.type | article | |
dc.identifier.urn | URN:NBN:fi:jyu-201610244425 | |
dc.contributor.laitos | Bio- ja ympäristötieteiden laitos | fi |
dc.contributor.laitos | Fysiikan laitos | fi |
dc.contributor.laitos | Kemian laitos | fi |
dc.contributor.laitos | Department of Biological and Environmental Science | en |
dc.contributor.laitos | Department of Physics | en |
dc.contributor.laitos | Department of Chemistry | en |
dc.contributor.oppiaine | Solu- ja molekyylibiologia | fi |
dc.contributor.oppiaine | Fysikaalinen kemia | fi |
dc.contributor.oppiaine | Nanoscience Center | fi |
dc.contributor.oppiaine | Cell and Molecular Biology | en |
dc.contributor.oppiaine | Physical Chemistry | en |
dc.contributor.oppiaine | Nanoscience Center | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.date.updated | 2016-10-24T12:15:11Z | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.format.pagerange | 35279 | |
dc.relation.issn | 2045-2322 | |
dc.relation.numberinseries | 0 | |
dc.relation.volume | 6 | |
dc.type.version | publishedVersion | |
dc.rights.copyright | © the Authors, 2016. This is an open access article distributed under a Creative Commons Attribution 4.0 International License. | |
dc.rights.accesslevel | openAccess | fi |
dc.subject.yso | kidetiede | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p643 | |
dc.rights.url | https://creativecommons.org/licenses/by/4.0/ | |
dc.relation.doi | 10.1038/srep35279 | |
dc.type.okm | A1 | |