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dc.contributor.authorPajari, Anne-Maria
dc.contributor.authorPäivärinta, Essi
dc.contributor.authorPaavolainen, Lassi
dc.contributor.authorVaara, Elina
dc.contributor.authorKoivumäki, Tuuli
dc.contributor.authorGarg, Ritu
dc.contributor.authorHeiman-Lindh, Anu
dc.contributor.authorMutanen, Marja
dc.contributor.authorMarjomäki, Varpu
dc.contributor.authorRidley, Anne J.
dc.date.accessioned2016-08-22T10:46:15Z
dc.date.available2016-08-22T10:46:15Z
dc.date.issued2016
dc.identifier.citationPajari, A.-M., Päivärinta, E., Paavolainen, L., Vaara, E., Koivumäki, T., Garg, R., Heiman-Lindh, A., Mutanen, M., Marjomäki, V., & Ridley, A. J. (2016). Ellagitannin-rich cloudberry inhibits hepatocyte growth factorinduced cell migration and phosphatidylinositol 3-kinase/AKT activation in colon carcinoma cells and tumors in Min mice. <i>Oncotarget</i>, <i>7</i>(28), 43907-43923. <a href="https://doi.org/10.18632/oncotarget.9724" target="_blank">https://doi.org/10.18632/oncotarget.9724</a>
dc.identifier.otherCONVID_26169810
dc.identifier.otherTUTKAID_70934
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/50989
dc.description.abstractBerries have been found to inhibit colon carcinogenesis in animal models, and thus represent a potential source of compounds for prevention and treatment of colorectal cancer. The mechanistic basis for their effects is not well understood. We used human colon carcinoma cells and Min mice to investigate the effects of ellagitannin-rich cloudberry (Rubus chamaemorus) extract on cancer cell migration and underlying cell signaling. Intrinsic and hepatocyte growth factor (HGF) -induced cell motility in human HT29 and HCA7 colon carcinoma cells was assessed carrying out cell scattering and scratch wound healing assays using time-lapse microscopy. Activation of Met, AKT, and ERK in cell lines and tumors of cloudberry-fed Min mice were determined using immunoprecipitation, Western blot and immunohistochemical analyses. Cloudberry extract significantly inhibited particularly HGF-induced cancer cell migration in both cell lines. Cloudberry extract inhibited the Met receptor tyrosine phosphorylation by HGF and strongly suppressed HGF-induced AKT and ERK activation in both HT29 and HCA7 cells. Consistently, cloudberry feeding (10% w/w freezedried berries in diet for 10 weeks) reduced the level of active AKT and prevented phosphoMet localization at the edges in tumors of Min mice. These results indicate that cloudberry reduces tumor growth and cancer cell motility by inhibiting Met signaling and consequent activation of phosphatidylinositol 3-kinase/AKT in vitro and in tumors in vivo. As the Met receptor is recognized to be a major target in cancer treatment, our results suggest that dietary phytochemicals may have therapeutic value in reducing cancer progression and metastasis.
dc.language.isoeng
dc.publisherImpact Journals LLC
dc.relation.ispartofseriesOncotarget
dc.subject.othercolorectal cancer
dc.subject.otherMet receptor
dc.subject.otherellagitannins
dc.subject.otherMin mouse
dc.titleEllagitannin-rich cloudberry inhibits hepatocyte growth factorinduced cell migration and phosphatidylinositol 3-kinase/AKT activation in colon carcinoma cells and tumors in Min mice
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201608173815
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2016-08-17T12:15:07Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.format.pagerange43907-43923
dc.relation.issn1949-2553
dc.relation.numberinseries28
dc.relation.volume7
dc.type.versionpublishedVersion
dc.rights.copyright© the Authors, 2016. This is an open access article distributed under the terms of a Creative Commons License.
dc.rights.accesslevelopenAccessfi
dc.subject.ysosolumigraatio
jyx.subject.urihttp://www.yso.fi/onto/yso/p38818
dc.rights.urlhttps://creativecommons.org/licenses/by/3.0/
dc.relation.doi10.18632/oncotarget.9724


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© the Authors, 2016. This is an open access article distributed under the terms of a Creative Commons License.
Except where otherwise noted, this item's license is described as © the Authors, 2016. This is an open access article distributed under the terms of a Creative Commons License.