Ellagitannin-rich cloudberry inhibits hepatocyte growth factorinduced cell migration and phosphatidylinositol 3-kinase/AKT activation in colon carcinoma cells and tumors in Min mice
Pajari, A.-M., Päivärinta, E., Paavolainen, L., Vaara, E., Koivumäki, T., Garg, R., Heiman-Lindh, A., Mutanen, M., Marjomäki, V., & Ridley, A. J. (2016). Ellagitannin-rich cloudberry inhibits hepatocyte growth factorinduced cell migration and phosphatidylinositol 3-kinase/AKT activation in colon carcinoma cells and tumors in Min mice. Oncotarget, 7(28), 43907-43923. https://doi.org/10.18632/oncotarget.9724
Published in
OncotargetAuthors
Date
2016Copyright
© the Authors, 2016. This is an open access article distributed under the terms of a Creative Commons License.
Berries have been found to inhibit colon carcinogenesis in animal models, and
thus represent a potential source of compounds for prevention and treatment of
colorectal cancer. The mechanistic basis for their effects is not well understood.
We used human colon carcinoma cells and Min mice to investigate the effects of
ellagitannin-rich cloudberry (Rubus chamaemorus) extract on cancer cell migration
and underlying cell signaling. Intrinsic and hepatocyte growth factor (HGF) -induced
cell motility in human HT29 and HCA7 colon carcinoma cells was assessed carrying
out cell scattering and scratch wound healing assays using time-lapse microscopy.
Activation of Met, AKT, and ERK in cell lines and tumors of cloudberry-fed Min mice
were determined using immunoprecipitation, Western blot and immunohistochemical
analyses. Cloudberry extract significantly inhibited particularly HGF-induced cancer
cell migration in both cell lines. Cloudberry extract inhibited the Met receptor tyrosine
phosphorylation by HGF and strongly suppressed HGF-induced AKT and ERK activation
in both HT29 and HCA7 cells. Consistently, cloudberry feeding (10% w/w freezedried
berries in diet for 10 weeks) reduced the level of active AKT and prevented
phosphoMet localization at the edges in tumors of Min mice. These results indicate
that cloudberry reduces tumor growth and cancer cell motility by inhibiting Met
signaling and consequent activation of phosphatidylinositol 3-kinase/AKT in vitro
and in tumors in vivo. As the Met receptor is recognized to be a major target in cancer
treatment, our results suggest that dietary phytochemicals may have therapeutic
value in reducing cancer progression and metastasis.
...


Publisher
Impact Journals LLCISSN Search the Publication Forum
1949-2553Publication in research information system
https://converis.jyu.fi/converis/portal/detail/Publication/26169810
Metadata
Show full item recordCollections
License
Except where otherwise noted, this item's license is described as © the Authors, 2016. This is an open access article distributed under the terms of a Creative Commons License.
Related items
Showing items with similar title or keywords.
-
EGFR gene copy number decreases during anti-EGFR antibody therapy in colorectal cancer
Birkman, Eva-Maria; Avoranta, Tuulia; Ålgars, Annika; Korkeila, Eija; Lintunen, Minnamaija; Lahtinen, Laura; Kuopio, Teijo; Ristamäki, Raija; Carpén, Olli; Sundström, Jari (Elsevier Inc., 2018)Epidermal growth factor receptor (EGFR) gene copy number (GCN) increase is associated with a favorable anti-EGFR antibody treatment response in RAS wild-type metastatic colorectal cancer. However, there are limited and ... -
Epidemiological, clinical and molecular characterization of Lynch-like syndrome : A population-based study
Porkka, Noora; Lahtinen, Laura; Ahtiainen, Maarit; Böhm, Jan P.; Kuopio, Teijo; Eldfors, Samuli; Mecklin, Jukka-Pekka; Seppälä, Toni T.; Peltomäki, Päivi (John Wiley & Sons, Inc., 2019)Colorectal carcinomas that are mismatch repair (MMR)‐deficient in the absence of MLH1 promoter methylation or germline mutations represent Lynch‐like syndrome (LLS). Double somatic events inactivating MMR genes are involved ... -
Lack of association between screening interval and cancer stage in Lynch syndrome may be accounted for by over-diagnosis; a prospective Lynch syndrome database report
Seppälä, Toni T.; Ahadova, Aysel; Dominguez-Valentin, Mev; Macrae, Finlay; Evans, D. Gareth; Therkildsen, Christina; Sampson, Julian; Scott, Rodney; Burn, John; Möslein, Gabriela; Bernstein, Inge; Holinski-Feder, Elke; Pylvänäinen, Kirsi; Renkonen-Sinisalo, Laura; Lepistö, Anna; Lautrup, Charlotte Kvist; Lindblom, Annika; Plazzer, John-Paul; Winship, Ingrid; Tjandra, Douglas; Katz, Lior H.; Aretz, Stefan; Hüneburg, Robert; Holzapfel, Stefanie; Heinimann, Karl; Valle, Adriana Della; Neffa, Florencia; Gluck, Nathan; Cappel, Wouter H. de Vos tot Nederveen; Vasen, Hans; Morak, Monika; Steinke-Lange, Verena; Engel, Christoph; Rahner, Nils; Schmiegel, Wolff; Vangala, Deepak; Thomas, Huw; Green, Kate; Lalloo, Fiona; Crosbie, Emma J.; Hill, James; Capella, Gabriel; Pineda, Marta; Navarro, Matilde; Blanco, Ignacio; Broeke, Sanne ten; Nielsen, Maartje; Ljungmann, Ken; Nakken, Sigve; Lindor, Noralane; Frayling, Ian; Hovig, Eivind; Sunde, Lone; Kloor, Matthias; Mecklin, Jukka-Pekka; Kalager, Mette; Møller, Pål (BioMed Central Ltd., 2019)Background Recent epidemiological evidence shows that colorectal cancer (CRC) continues to occur in carriers of pathogenic mismatch repair (path_MMR) variants despite frequent colonoscopy surveillance in expert centres. ... -
Tissue microarray technology in breast cancer subtyping
Nieminen, Anssi (2008) -
Blocking Activin Receptor Ligands Is Not Sufficient to Rescue Cancer-Associated Gut Microbiota : A Role for Gut Microbial Flagellin in Colorectal Cancer and Cachexia?
Pekkala, Satu; Keskitalo, Anniina; Kettunen, Emilia; Lensu, Sanna; Nykänen, Noora; Kuopio, Teijo; Ritvos, Olli; Hentilä, Jaakko; Nissinen, Tuuli A.; Hulmi, Juha J. (MDPI AG, 2019)Colorectal cancer (CRC) and cachexia are associated with the gut microbiota and microbial surface molecules. We characterized the CRC-associated microbiota and investigated whether cachexia affects the microbiota composition. ...