dc.contributor.author | Hynninen, Ville | |
dc.contributor.author | Vuori, Leena | |
dc.contributor.author | Hannula, Markku | |
dc.contributor.author | Tapio, Kosti | |
dc.contributor.author | Lahtonen, Kimmo | |
dc.contributor.author | Isoniemi, Tommi | |
dc.contributor.author | Lehtonen, Elina | |
dc.contributor.author | Hirsimäki, Mika | |
dc.contributor.author | Toppari, Jussi | |
dc.contributor.author | Valden, Mika | |
dc.contributor.author | Hytönen, Vesa P. | |
dc.date.accessioned | 2016-07-08T04:55:01Z | |
dc.date.available | 2016-07-08T04:55:01Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Hynninen, V., Vuori, L., Hannula, M., Tapio, K., Lahtonen, K., Isoniemi, T., Lehtonen, E., Hirsimäki, M., Toppari, J., Valden, M., & Hytönen, V. P. (2016). Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology. <i>Scientific Reports</i>, <i>6</i>, Article 29324. <a href="https://doi.org/10.1038/srep29324" target="_blank">https://doi.org/10.1038/srep29324</a> | |
dc.identifier.other | CONVID_26104880 | |
dc.identifier.other | TUTKAID_70578 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/50765 | |
dc.description.abstract | A straightforward solution-based method to modify the biofunctionality of stainless steel (SS) using heterobifunctional silane-polyethylene glycol (silane-PEG) overlayers is reported. Reduced nonspecific biofouling of both proteins and bacteria onto SS and further selective biofunctionalization of the modified surface were achieved. According to photoelectron spectroscopy analyses, the silane-PEGs formed less than 10 Å thick overlayers with close to 90% surface coverage and reproducible chemical compositions. Consequently, the surfaces also became more hydrophilic, and the observed non-specific biofouling of proteins was reduced by approximately 70%. In addition, the attachment of E. coli was reduced by more than 65%. Moreover, the potential of the overlayer to be further modified was demonstrated by successfully coupling biotinylated alkaline phosphatase (bAP) to a silane-PEG-biotin overlayer via avidin-biotin bridges. The activity of the immobilized enzyme was shown to be well preserved without compromising the achieved antifouling properties. Overall, the simple solution-based approach enables the tailoring of SS to enhance its activity for biomedical and biotechnological applications. | |
dc.language.iso | eng | |
dc.publisher | Nature Publishing Group | |
dc.relation.ispartofseries | Scientific Reports | |
dc.subject.other | biofunctionality | |
dc.title | Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology | |
dc.type | article | |
dc.identifier.urn | URN:NBN:fi:jyu-201607073528 | |
dc.contributor.laitos | Fysiikan laitos | fi |
dc.contributor.laitos | Department of Physics | en |
dc.contributor.oppiaine | Nanoscience Center | fi |
dc.contributor.oppiaine | Nanoscience Center | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.date.updated | 2016-07-07T12:15:17Z | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.relation.issn | 2045-2322 | |
dc.relation.numberinseries | 0 | |
dc.relation.volume | 6 | |
dc.type.version | publishedVersion | |
dc.rights.copyright | © the Authors, 2016. This is an open access article published by Nature and distributed under the terms of a Creative Commons Attribution 4.0 International License. | |
dc.rights.accesslevel | openAccess | fi |
dc.subject.yso | ruostumaton teräs | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p15964 | |
dc.rights.url | https://creativecommons.org/licenses/by/4.0/ | |
dc.relation.doi | 10.1038/srep29324 | |
dc.type.okm | A1 | |