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dc.contributor.authorHynninen, Ville
dc.contributor.authorVuori, Leena
dc.contributor.authorHannula, Markku
dc.contributor.authorTapio, Kosti
dc.contributor.authorLahtonen, Kimmo
dc.contributor.authorIsoniemi, Tommi
dc.contributor.authorLehtonen, Elina
dc.contributor.authorHirsimäki, Mika
dc.contributor.authorToppari, Jussi
dc.contributor.authorValden, Mika
dc.contributor.authorHytönen, Vesa P.
dc.date.accessioned2016-07-08T04:55:01Z
dc.date.available2016-07-08T04:55:01Z
dc.date.issued2016
dc.identifier.citationHynninen, V., Vuori, L., Hannula, M., Tapio, K., Lahtonen, K., Isoniemi, T., Lehtonen, E., Hirsimäki, M., Toppari, J., Valden, M., & Hytönen, V. P. (2016). Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology. <i>Scientific Reports</i>, <i>6</i>, Article 29324. <a href="https://doi.org/10.1038/srep29324" target="_blank">https://doi.org/10.1038/srep29324</a>
dc.identifier.otherCONVID_26104880
dc.identifier.otherTUTKAID_70578
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/50765
dc.description.abstractA straightforward solution-based method to modify the biofunctionality of stainless steel (SS) using heterobifunctional silane-polyethylene glycol (silane-PEG) overlayers is reported. Reduced nonspecific biofouling of both proteins and bacteria onto SS and further selective biofunctionalization of the modified surface were achieved. According to photoelectron spectroscopy analyses, the silane-PEGs formed less than 10 Å thick overlayers with close to 90% surface coverage and reproducible chemical compositions. Consequently, the surfaces also became more hydrophilic, and the observed non-specific biofouling of proteins was reduced by approximately 70%. In addition, the attachment of E. coli was reduced by more than 65%. Moreover, the potential of the overlayer to be further modified was demonstrated by successfully coupling biotinylated alkaline phosphatase (bAP) to a silane-PEG-biotin overlayer via avidin-biotin bridges. The activity of the immobilized enzyme was shown to be well preserved without compromising the achieved antifouling properties. Overall, the simple solution-based approach enables the tailoring of SS to enhance its activity for biomedical and biotechnological applications.
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofseriesScientific Reports
dc.subject.otherbiofunctionality
dc.titleImproved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201607073528
dc.contributor.laitosFysiikan laitosfi
dc.contributor.laitosDepartment of Physicsen
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2016-07-07T12:15:17Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.relation.issn2045-2322
dc.relation.numberinseries0
dc.relation.volume6
dc.type.versionpublishedVersion
dc.rights.copyright© the Authors, 2016. This is an open access article published by Nature and distributed under the terms of a Creative Commons Attribution 4.0 International License.
dc.rights.accesslevelopenAccessfi
dc.subject.ysoruostumaton teräs
jyx.subject.urihttp://www.yso.fi/onto/yso/p15964
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1038/srep29324


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© the Authors, 2016. This is an open access article published by Nature and distributed under the terms of a Creative Commons Attribution 4.0 International License.
Except where otherwise noted, this item's license is described as © the Authors, 2016. This is an open access article published by Nature and distributed under the terms of a Creative Commons Attribution 4.0 International License.