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dc.contributor.authorMattila, Sari
dc.contributor.authorRuotsalainen, Pilvi
dc.contributor.authorJalasvuori, Matti
dc.date.accessioned2016-01-04T08:11:54Z
dc.date.available2016-01-04T08:11:54Z
dc.date.issued2015
dc.identifier.citationMattila, S., Ruotsalainen, P., & Jalasvuori, M. (2015). On-Demand Isolation of Bacteriophages Against Drug-Resistant Bacteria for Personalized Phage Therapy. <i>Frontiers in Microbiology</i>, <i>6</i>, Article 1271. <a href="https://doi.org/10.3389/fmicb.2015.01271" target="_blank">https://doi.org/10.3389/fmicb.2015.01271</a>
dc.identifier.otherCONVID_25382344
dc.identifier.otherTUTKAID_68269
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/48227
dc.description.abstractBacteriophages are bacterial viruses, capable of killing even multi-drug resistant bacterial cells. For this reason, therapeutic use of phages is considered as a possible alternative to conventional antibiotics. However, phages are very host specific in comparison to wide-spectrum antibiotics and thus preparation of phage-cocktails beforehand against pathogens can be difficult. In this study, we evaluate whether it may be possible to isolate phages on-demand from environmental reservoir. We attempted to enrich infectious bacteriophages from sewage against nosocomial drug-resistant bacterial strains of different medically important species in order to evaluate the probability of discovering novel therapeutic phages. Stability and host-range were determined for the acquired phages. Our results suggest that on-demand isolation of phages is possible against Pseudomonas aeruginosa, Salmonella and extended spectrum beta-lactamase Escherichia coli and Klebsiella pneumoniae. The probability of finding suitable phages was less than 40% against vancomycin resistant Enterococcus and Acinetobacter baumannii strains. Furthermore, isolation of new phages against methicillin resistant Staphylococcus aureus strains was found to be very difficult.
dc.language.isoeng
dc.publisherFrontiers Research Foundation
dc.relation.ispartofseriesFrontiers in Microbiology
dc.subject.otherESBL
dc.subject.otherphage cocktails
dc.titleOn-Demand Isolation of Bacteriophages Against Drug-Resistant Bacteria for Personalized Phage Therapy
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201512294139
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineBiologisten vuorovaikutusten huippututkimusyksikköfi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineCentre of Excellence in Biological Interactions Researchen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2015-12-29T10:15:20Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.relation.issn1664-302X
dc.relation.numberinseries0
dc.relation.volume6
dc.type.versionpublishedVersion
dc.rights.copyright© 2015 Mattila, Ruotsalainen and Jalasvuori. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
dc.rights.accesslevelopenAccessfi
dc.subject.ysoantibioottiresistenssi
dc.subject.ysoMRSA
dc.subject.ysofagiterapia
dc.subject.ysobakteriofagit
jyx.subject.urihttp://www.yso.fi/onto/yso/p29640
jyx.subject.urihttp://www.yso.fi/onto/yso/p23013
jyx.subject.urihttp://www.yso.fi/onto/yso/p29496
jyx.subject.urihttp://www.yso.fi/onto/yso/p25303
dc.rights.urlhttp://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3389/fmicb.2015.01271


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© 2015 Mattila, Ruotsalainen and Jalasvuori. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
Except where otherwise noted, this item's license is described as © 2015 Mattila, Ruotsalainen and Jalasvuori. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).