dc.contributor.author | Mattila, Sari | |
dc.contributor.author | Ruotsalainen, Pilvi | |
dc.contributor.author | Jalasvuori, Matti | |
dc.date.accessioned | 2016-01-04T08:11:54Z | |
dc.date.available | 2016-01-04T08:11:54Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Mattila, S., Ruotsalainen, P., & Jalasvuori, M. (2015). On-Demand Isolation of Bacteriophages Against Drug-Resistant Bacteria for Personalized Phage Therapy. <i>Frontiers in Microbiology</i>, <i>6</i>, Article 1271. <a href="https://doi.org/10.3389/fmicb.2015.01271" target="_blank">https://doi.org/10.3389/fmicb.2015.01271</a> | |
dc.identifier.other | CONVID_25382344 | |
dc.identifier.other | TUTKAID_68269 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/48227 | |
dc.description.abstract | Bacteriophages are bacterial viruses, capable of killing even multi-drug resistant bacterial cells. For this reason, therapeutic use of phages is considered as a possible alternative to conventional antibiotics. However, phages are very host specific in comparison to wide-spectrum antibiotics and thus preparation of phage-cocktails beforehand against pathogens can be difficult. In this study, we evaluate whether it may be possible to isolate phages on-demand from environmental reservoir. We attempted to enrich infectious bacteriophages from sewage against nosocomial drug-resistant bacterial strains of different medically important species in order to evaluate the probability of discovering novel therapeutic phages. Stability and host-range were determined for the acquired phages. Our results suggest that on-demand isolation of phages is possible against Pseudomonas aeruginosa, Salmonella and extended spectrum beta-lactamase Escherichia coli and Klebsiella pneumoniae. The probability of finding suitable phages was less than 40% against vancomycin resistant Enterococcus and Acinetobacter baumannii strains. Furthermore, isolation of new phages against methicillin resistant Staphylococcus aureus strains was found to be very difficult. | |
dc.language.iso | eng | |
dc.publisher | Frontiers Research Foundation | |
dc.relation.ispartofseries | Frontiers in Microbiology | |
dc.subject.other | ESBL | |
dc.subject.other | phage cocktails | |
dc.title | On-Demand Isolation of Bacteriophages Against Drug-Resistant Bacteria for Personalized Phage Therapy | |
dc.type | article | |
dc.identifier.urn | URN:NBN:fi:jyu-201512294139 | |
dc.contributor.laitos | Bio- ja ympäristötieteiden laitos | fi |
dc.contributor.laitos | Department of Biological and Environmental Science | en |
dc.contributor.oppiaine | Solu- ja molekyylibiologia | fi |
dc.contributor.oppiaine | Biologisten vuorovaikutusten huippututkimusyksikkö | fi |
dc.contributor.oppiaine | Cell and Molecular Biology | en |
dc.contributor.oppiaine | Centre of Excellence in Biological Interactions Research | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.date.updated | 2015-12-29T10:15:20Z | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.relation.issn | 1664-302X | |
dc.relation.numberinseries | 0 | |
dc.relation.volume | 6 | |
dc.type.version | publishedVersion | |
dc.rights.copyright | © 2015 Mattila, Ruotsalainen and Jalasvuori. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). | |
dc.rights.accesslevel | openAccess | fi |
dc.subject.yso | antibioottiresistenssi | |
dc.subject.yso | MRSA | |
dc.subject.yso | fagiterapia | |
dc.subject.yso | bakteriofagit | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p29640 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p23013 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p29496 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p25303 | |
dc.rights.url | http://creativecommons.org/licenses/by/4.0/ | |
dc.relation.doi | 10.3389/fmicb.2015.01271 | |
dc.type.okm | A1 | |