Seasonal gene expression kinetics between diapause phases in Drosophila virilis group species and overwintering differences between diapausing and non-diapausing females
Salminen, T., Vesala, L., Laiho, A., Merisalo, M., Hoikkala, A., & Kankare, M. (2015). Seasonal gene expression kinetics between diapause phases in Drosophila virilis group species and overwintering differences between diapausing and non-diapausing females. Scientific Reports, 5, Article 11197. https://doi.org/10.1038/srep11197
Published inScientific Reports
© 2015 the Authors. The article has been distributed under a Creative Commons CC-BY license. Published by Nature Publishing Group.
Most northern insect species experience a period of developmental arrest, diapause, which enables them to survive over the winter and postpone reproduction until favorable conditions. We studied the timing of reproductive diapause and its long-term effects on the cold tolerance of Drosophila montana, D. littoralis and D. ezoana females in seasonally varying environmental conditions. At the same time we traced expression levels of 219 genes in D. montana using a custom-made microarray. We show that the seasonal switch to reproductive diapause occurs over a short time period, and that overwintering in reproductive diapause has long-lasting effects on cold tolerance. Some genes, such as Hsc70, Jon25Bi and period, were upregulated throughout the diapause, while others, including regucalcin, couch potato and Thor, were upregulated only at its specific phases. Some of the expression patterns induced during the sensitive stage, when the females either enter diapause or not, remained induced regardless of the later conditions. qPCR analyses confirmed the findings of the microarray analysis in D. montana and revealed similar gene expression changes in D. littoralis and D. ezoana. The present study helps to achieve a better understanding of the genetic regulation of diapause and of the plasticity of seasonal responses in general. ...
PublisherNature Publishing Group
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Related funder(s)Academy of Finland
Funding program(s)Academy Research Fellow, AoF
Additional information about fundingWe thank several colleagues for their very useful discussions and suggestions. The work has been supported by The Finnish Centre of Excellence in Evolutionary Research, by the Academy of Finland to Anneli Hoikkala (project 132619) and to Maaria Kankare (project 268214) and by the Finnish Cultural Foundation to Tiina S. Salminen.
Except where otherwise noted, this item's license is described as © 2015 the Authors. The article has been distributed under a Creative Commons CC-BY license. Published by Nature Publishing Group.
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