Transcriptional Differences between Diapausing and Non-Diapausing D. montana Females Reared under the Same Photoperiod and Temperature
Kankare, M., Parker, D., Merisalo, M., Salminen, T. S., & Hoikkala, A. (2016). Transcriptional Differences between Diapausing and Non-Diapausing D. montana Females Reared under the Same Photoperiod and Temperature. PLoS ONE, 11(8), Article e0161852. https://doi.org/10.1371/journal.pone.0161852
Published in
PLoS ONEDate
2016Copyright
© 2016 Kankare et al. This is an open
access article distributed under the terms of the
Creative Commons Attribution License.
Background
A wide range of insects living at higher latitudes enter diapause at the end of the warm season,
which increases their chances of survival through harsh winter conditions. In this study
we used RNA sequencing to identify genes involved in adult reproductive diapause in a
northern fly species, Drosophila montana. Both diapausing and non-diapausing flies were
reared under a critical day length and temperature, where about half of the emerging
females enter diapause enabling us to eliminate the effects of varying environmental conditions
on gene expression patterns of the two types of female flies.
Results
RNA sequencing revealed large differences between gene expression patterns of diapausing
and non-diapausing females, especially in genes involved with metabolism, fatty acid
biosynthesis, and metal and nucleotide binding. Differently expressed genes included several
gene groups, including myosin, actin and cytochromeP450 genes, which have been
previously associated with diapause. This study also identified new candidate genes,
including some involved in cuticular hydrocarbon synthesis or regulation (desat1 and
desat2), and acyl-CoA Δ11-desaturase activity (CG9747), and few odorant-binding protein
genes (e.g. Obp44A). Also, several transposable elements (TEs) showed differential
expression between the two female groups motivating future research on their roles in
diapause.
Conclusions
Our results demonstrate that the adult reproductive diapause in D. montana involves
changes in the expression level of a variety of genes involved in key processes (e.g. metabolism and fatty acid biosynthesis) which help diapausing females to cope with overwintering.
This is consistent with the view that diapause is a complex adaptive phenotype
where not only sexual maturation is arrested, but also changes in adult physiology are
required in order to survive over the winter.
...
Publisher
Public Library of ScienceISSN Search the Publication Forum
1932-6203Publication in research information system
https://converis.jyu.fi/converis/portal/detail/Publication/26198076
Metadata
Show full item recordCollections
License
Except where otherwise noted, this item's license is described as © 2016 Kankare et al. This is an open
access article distributed under the terms of the
Creative Commons Attribution License.
Related items
Showing items with similar title or keywords.
-
Clinal variation in the temperature and photoperiodic control of reproductive diapause in Drosophila montana females
Lankinen, Pekka; Kastally, Chedly; Hoikkala, Anneli (Elsevier, 2023)Insect adaptation to climatic conditions at different latitudes has required changes in life-history traits linked with survival and reproduction. Several species, including Drosophila montana, show robust latitudinal ... -
Preparing for Winter: The Transcriptomic Response Associated with Different Day Lengths in Drosophila montana
Parker, Darren; Ritchie, Michael G.; Kankare, Maaria (Genetics Society of America, 2016)At northern latitudes, the most robust cue for assessing the onset of winter is the shortening of day lengths. Many species use day length as a cue to increase their cold tolerance and/or enter into diapause, but little ... -
Plasticity in Photoperiodism : Drosophila montana Females Have a Life-Long Ability to Switch From Reproduction to Diapause
Lankinen, Pekka; Kastally, Chedly; Hoikkala, Anneli (SAGE Publications, 2022)Photoperiodic reproductive diapause is an essential part of female life cycle in several insect species living on high latitudes, where overwintering in reproductive stage involves high risks for survival and progeny ... -
Nanda-Hamner Curves Show Huge Latitudinal Variation but No Circadian Components in Drosophila Montana Photoperiodism
Lankinen, Pekka, Kastally, Chedly, Hoikkala, Anneli (SAGE Publications, 2021)Insect species with a wide distribution offer a great opportunity to trace latitudinal variation in the photoperiodic regulation of traits important in reproduction and stress tolerances. We measured this variation in the ... -
Latitudinal clines in the timing and temperature‐sensitivity of photoperiodic reproductive diapause in Drosophila montana
Tyukmaeva, Venera; Lankinen, Pekka; Kinnunen, Johanna; Kauranen, Hannele; Hoikkala, Anneli (John Wiley & Sons, 2020)Reproductive diapause is a primary mechanism used by arthropods to synchronize their life cycle with seasonal changes in temperate regions. Our study species, Drosophila montana, represents the northern insect species where ...