Comparing the different morphotypes of a fish pathogen - implications for key virulence factors in Flavobacterium columnare
Lataukset:
Laanto, E., Penttinen, R., Bamford, J., & Sundberg, L.-R. (2014). Comparing the different morphotypes of a fish pathogen - implications for key virulence factors in Flavobacterium columnare. BMC Microbiology, 14(170), 1-11. https://doi.org/10.1186/1471-2180-14-170
Julkaistu sarjassa
BMC MicrobiologyPäivämäärä
2014Tekijänoikeudet
© 2014 Laanto et al.; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver applies to the data made available in this article, unless otherwise stated.
Background:
Flavobacterium columnare
(Bacteroidetes) is the causative agent of columnaris disease in farmed
freshwater fish around the world. The bacterium forms three colony morphotypes (Rhizoid, Rough and Soft), but
the differences of the morphotypes are poorly known. We studied the virulence of the morphotypes produced
by
F. columnare
strain B067 in rainbow trout (
Onconrhynchus mykiss
) and used high-resolution scanning electron
microscopy to identify the fine structures of the cells grown in liquid and on agar. We also analysed the proteins
secreted extracellularly and in membrane vesicles to identify possible virulence factors.
Results:
Only the Rhizoid morphotype was virulent in rainbow trout. Under electron microscopy, the cells of
Rhizoid and Soft morphotypes were observed to display an organised structure within the colony, whereas in the
Rough type this internal organisation was absent. Planktonic cells of the Rhizoid and Rough morphotypes produced
large membrane vesicles that were not seen on the cells of the Soft morphotype. The vesicles were purified and
analysed. Two proteins with predicted functions were identified, OmpA and SprF. Furthermore, the Rhizoid
morphotype secreted a notable amount of a small, unidentified 13 kDa protein absent in the Rough and Soft
morphotypes, indicating an association with bacterial virulence.
Conclusions:
Our results suggest three factors that are associated with the virulence of
F. columnare
: the
coordinated organisation of cells, a secreted protein and outer membrane vesicles. The internal organisation of the
cells within a colony may be associated with bacterial gliding motility, which has been suggested to be connected
with virulence in
F. columnare
. The function of the secreted 13 kDa protein by the cells of the virulent morphotype
cells remains unknown. The membrane vesicles might be connected with the adhesion of cells to the surfaces and
could also carry potential virulence factors. Indeed, OmpA is a virulence factor in several bacterial pathogens, often
linked with adhesion and invasion, and SprF is a protein connected with gliding motility and the protein secretion
of flavobacteria.
...
Julkaisija
BioMed Central Ltd.ISSN Hae Julkaisufoorumista
1471-2180Asiasanat
Alkuperäislähde
http://www.biomedcentral.com/1471-2180/14/170Julkaisu tutkimustietojärjestelmässä
https://converis.jyu.fi/converis/portal/detail/Publication/23769402
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Lisenssi
Ellei muuten mainita, aineiston lisenssi on © 2014 Laanto et al.; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver applies to the data made available in this article, unless otherwise stated.
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