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dc.contributor.authorTurkki, Paula
dc.contributor.authorMakkonen, Emilia
dc.contributor.authorHuttunen, Moona
dc.contributor.authorLaakkonen, Johanna
dc.contributor.authorYläHerttuala, Seppo
dc.contributor.authorAirenne, Kari
dc.contributor.authorMarjomäki, Varpu
dc.date.accessioned2013-10-07T08:13:17Z
dc.date.available2014-01-03T22:45:05Z
dc.date.issued2013
dc.identifier.citationTurkki, P., Makkonen, E., Huttunen, M., Laakkonen, J., YläHerttuala, S., Airenne, K., & Marjomäki, V. (2013). Cell susceptibility to baculovirus transduction and echovirus infection is modified by protein kinase C phosphorylation and vimentin organization.. <i>Journal of Virology</i>, <i>87</i>(17), 9822-9835. <a href="https://doi.org/10.1128/JVI.01004-13" target="_blank">https://doi.org/10.1128/JVI.01004-13</a>
dc.identifier.otherCONVID_22538859
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/42294
dc.description.abstractSome cell types are more susceptible to viral gene transfer or virus infection than others, irrespective of the number of viral receptors or virus binding efficacy on their surfaces. In order to characterize the cell-line-specific features contributing to efficient virus entry, we studied two cell lines (Ea.hy926 and MG-63) that are nearly nonpermissive to insect-specific baculovirus (BV) and the human enterovirus echovirus 1 (EV1) and compared their characteristics with those of a highly permissive (HepG2) cell line. All the cell lines contained high levels of viral receptors on their surfaces, and virus binding was shown to be efficient. However, in nonpermissive cells, BV and its receptor, syndecan 1, were unable to internalize in the cells and formed large aggregates near the cell surface. Accordingly, EV1 had a low infection rate in nonpermissive cells but was still able to internalize the cells, suggesting that the postinternalization step of the virus was impaired. The nonpermissive and permissive cell lines showed differential expression of syntenin, filamentous actin, vimentin, and phosphorylated protein kinase C subtype α (pPKCα). The nonpermissive nature of the cells could be modulated by the choice of culture medium. RPMI medium could partially rescue infection/transduction and concomitantly showed lower syntenin expression, a modified vimentin network, and altered activities of PKC subtypes PKCα and PKCε. The observed changes in PKCα and PKCε activation caused alterations in the vimentin organization, leading to efficient BV transduction and EV1 infection. This study identifies PKCα, PKCε, and vimentin as key factors affecting efficient infection and transduction by EV1 and BV, respectively.fi
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofseriesJournal of Virology
dc.relation.urihttp://dx.doi.org/10.1128/JVI.01004-13
dc.subject.othervirologiafi
dc.subject.othervirologyen
dc.titleCell susceptibility to baculovirus transduction and echovirus infection is modified by protein kinase C phosphorylation and vimentin organization.
dc.typeresearch article
dc.identifier.urnURN:NBN:fi:jyu-201310032405
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2013-10-03T03:30:29Z
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.format.pagerange9822-9835
dc.relation.issn0022-538X
dc.relation.numberinseries17
dc.relation.volume87
dc.type.versionpublishedVersion
dc.rights.copyright© 2013 American Society for Microbiology
dc.rights.accesslevelopenAccessfi
dc.type.publicationarticle
dc.subject.ysovirologia
jyx.subject.urihttp://www.yso.fi/onto/yso/p14570
dc.relation.doi10.1128/JVI.01004-13
dc.type.okmA1


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