Chirality-controlled preparation and single molecule characterisation of carbon nanotubes
Nanostructures have gained increasing attention not only for their basic scientific richness, but also because they promise novelties and potentials that may lead to technological revolution. Carbon nanotubes (CNT’s) are one of the primary focuses of nanotechnology because of their unique physical properties such as huge Young modulus, high tensile strength, thermal and electrical conductivity. However many of their physical properties are extremely sensitive to their atomic structure. In order to optimize utilization of CNTs, their fundamental material properties should be well understood. A single shell of a CNT is composed of a wrapped up sheet of graphite. Depending on the wrapping, single wall carbon nanotubes (SWCNT) may have numerous different chiralities. Owing to SWCNT’s outstanding mechanical and electrical properties, many of their fundamental studies and technological applications require a population of tubes with identical chiralities. Separating SWCNT of different chiralities from each other is, however, a challenging problem. One proposed solution is to construct DNA-SWCNT hybrid and separate SWCNTs by their chiralities and diameter chromatographically. An oligomer of specific sequence of single-stranded DNA (ssDNA) has been shown to wrap around a SWCNT of a specific chirality and form ssDNA-SWCNT hybrid. Additionally self assembled DNA-SWCNT hybrids can be used a unique platform to study nucleic acid folding structures and other biophysical properties.
In this study, DNA-SWCNT hybrids were produced and sorted according to the chirality of the CNT in a controlled fashion by ion exchange chromatography (IEX). During the chromatography, eluent were collected in a series of fractions whose composition was monitored by HPLC detector. Selected fractions were then subjected to detailed analysis by UV-Vis to prove that the single chirality purification of DNA-SWCNT was successful. For confirmation, Atomic Force Microscopy was conducted on the samples and DNA wrapped SWCNTs with expected diameter and DNA pitch were observed indicating that the spectral signatures recorded in UV-Vis analysis is indeed coming from sorted DNA-SWCNT.
In future DNA-SWCNT hybrids will be deposited on an advanced nanofabricated silicon chip containing tiny openings. TEM measurements including DNA supertwist imaging and CNT chirality investigations will be done for direct determination of chiral indices (n,m) of the SWCNTs from their electron diffraction patterns (EDPs).
...
![showless](/themes/JYX2//images/showless.png)
![showmore](/themes/JYX2//images/showmore.png)
Metadata
Show full item recordCollections
- Pro gradu -tutkielmat [29041]
Related items
Showing items with similar title or keywords.
-
Preparation of novel [Co(SCN)4] hybrid material : crystal structure investigation, DFT mechanistic analysis, antioxidant activity, and molecular docking study for potential inflammatory disorder control
Makhlouf, Jawher; Louis, Hitler; Benjamin, Innocent; Amodu, Ismail O.; Valkonen, Arto; Abuelizz, Hatem A.; El Bakri, Youness; Al-Salahi, Rashad; Smirani Sta, Wajda (Taylor & Francis, 2024)The crystal structure of the title solvated coordination compound, [Co CN)4(BtaH)2]·(BtaH)2.6(H2O), consists of discrete complexes in which the Co2+cations are sixfold coordinated by four N-bonded thiocyanate anions and ... -
Preparation of potentially porous, chiral organometallic materials through spontaneous resolution of pincer palladium conformers
Johnson, Magnus T.; Dzolic, Zoran; Cetina, Mario; Lahtinen, Manu; Ahlquist, Mårten S. G.; Rissanen, Kari; Öhrström, Lars; Wendt, Ola F. (Royal Society of Chemistry, 2013)Understanding the mechanism by which advanced materials assemble is essential for the design of new materials with desired properties. Here, we report a method to form chiral, potentially porous materials through spontaneous ... -
Ca-P-O thin film preparation, modification and characterisation
Arcot Rajashekar, Ananda Sagari (University of Jyväskylä, 2011)Bioceramics are biocompatible ceramic materials, that interact with biological systems of the body to treat, strengthen or replace body functions. Most conventional bioceramics are oxide ceramics, glass ceramics and ... -
Structural characterisation of wood pulp fibres and their nanofibrillated materials for biodegradable composite applications
Chinga-Carrasco, Gary; Miettinen, Arttu; Luengo Hendriks, Cris. L.; Gamstedt, E. Kristofer; Kataja, Markku (InTech, 2011)Introduction: The utilization of wood pulp fibres in composite materials has gained major interest during the last years. One of the major motivations has been the potential of wood pulp fibres and their nanofibrillated ... -
Lignin-carbohydrate complexes of wood and non-wood materials : fractionation and characterisation
Mälkiä, Kia (2023)Nykytutkimuksen valossa ligniinin oletetaan olevan sitoutunut kovalenttisesti hiilihydraatteihin lignoselluloosapitoisessa biomassassa. Näitä rakenteita kutsutaan ligniini-hiilihydraatti komplekseiksi. Tämän pro gradu- ...