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dc.contributor.authorAhmed, Syeda Josna
dc.date.accessioned2024-07-15T05:13:02Z
dc.date.available2024-07-15T05:13:02Z
dc.date.issued2024
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/96420
dc.description.abstractPerceiving light is a crucial step for all photosynthetic organisms. This perception of light is mediated by a group of photoreceptor proteins called phytochrome that exhibit high responsiveness to light and govern other biological processes like growth and development in plants, bacteria, fungi, algae, and cyanobacteria. They utilize red light and far-red light to regulate different light-dependent cellular processes. Certain cyanobacteria contain a distinct phytochrome called RfpA, which enables them to carry out photosynthesis by harnessing far-red light. So far there is no structural-functional relationship is known about this protein. The aim of this study was to purify RfpA to explore its signaling mechanism and spectral characterization to observe the photocycle of the phytochrome. Due to the difficulties in the purification of full-length protein, we constructed three different versions of the protein varying in length and containing different domains (GAF-PHY-PAS, GAF-PHY, and GAF only) using site-directed mutagenesis. Although our primary goal was the purification of the full-length protein, we attempted to obtain the longest possible fragment of the protein. We successfully managed to purify GAF-PHY and GAF mutants by affinity and size exclusion chromatography. Furthermore, UV-Vis spectroscopy was performed to study the photocycle of the protein. Spectroscopy study highlights that RfpA protein exists predominantly in Pfr (Far-red light absorbing form) as its resting state, denoting the characteristics of a bathy phytochrome. These findings provide remarkable insight into the unique spectral properties of RfpA, which can be beneficial in developing optogenetic tools and biotechnological applications.en
dc.format.extent38
dc.language.isoen
dc.subject.otherRfpA phytochrome
dc.titlePurification and characterization of the RfpA phytochrome of a thermophilic cyanobacterium
dc.identifier.urnURN:NBN:fi:jyu-202407155246
dc.type.ontasotMaster’s thesisen
dc.type.ontasotPro gradu -tutkielmafi
dc.contributor.tiedekuntaMatemaattis-luonnontieteellinen tiedekuntafi
dc.contributor.tiedekuntaFaculty of Sciencesen
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.yliopistoJyväskylän yliopistofi
dc.contributor.yliopistoUniversity of Jyväskyläen
dc.contributor.oppiaineEkologia ja evoluutiobiologiafi
dc.contributor.oppiaineEcology and evolutionary biologyen
dc.rights.copyrightJulkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.fi
dc.rights.copyrightThis publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.en
dc.contributor.oppiainekoodi4011
dc.subject.ysoproteiinit
dc.subject.ysosyanobakteerit
dc.subject.ysoyhteyttäminen
dc.subject.ysoproteins
dc.subject.ysocyanobacteria
dc.subject.ysophotosynthesis


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