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dc.contributor.authorParikka, Johannes M.
dc.contributor.authorJärvinen, Heini
dc.contributor.authorSokołowska, Karolina
dc.contributor.authorRuokolainen, Visa
dc.contributor.authorMarkešević, Nemanja
dc.contributor.authorNatarajan, Ashwin K.
dc.contributor.authorVihinen-Ranta, Maija
dc.contributor.authorKuzyk, Anton
dc.contributor.authorTapio, Kosti
dc.contributor.authorToppari, J. Jussi
dc.date.accessioned2023-04-26T05:29:48Z
dc.date.available2023-04-26T05:29:48Z
dc.date.issued2023
dc.identifier.citationParikka, J. M., Järvinen, H., Sokołowska, K., Ruokolainen, V., Markešević, N., Natarajan, A. K., Vihinen-Ranta, M., Kuzyk, A., Tapio, K., & Toppari, J. J. (2023). Creation of ordered 3D tubes out of DNA origami lattices. <i>Nanoscale</i>, <i>15</i>(17), 7772-7780. <a href="https://doi.org/10.1039/D2NR06001A" target="_blank">https://doi.org/10.1039/D2NR06001A</a>
dc.identifier.otherCONVID_182874691
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/86593
dc.description.abstractHierarchical self-assembly of nanostructures with addressable complexity has been a promising route for realizing novel functional materials. Traditionally, the fabrication of such structures on a large scale has been achievable using top-down methods but with the cost of complexity of the fabrication equipment versus resolution and limitation mainly to 2D structures. More recently bottom-up methods using molecules like DNA have gained attention due to the advantages of low fabrication costs, high resolution and simplicity in an extension of the methods to the third dimension. One of the more promising bottom-up techniques is DNA origami due to the robust self-assembly of arbitrarily shaped nanostructures with feature sizes down to a few nanometers. Here, we show that under specific ionic conditions of the buffer, the employed plus-shaped, blunt-ended Seeman tile (ST) origami forms elongated, ordered 2D lattices, which are further rolled into 3D tubes in solution. Imaging structures on a surface by atomic force microscopy reveals ribbon-like structures, with single or double layers of the origami lattice. Further studies of the double-layered structures in a liquid state by confocal microscopy and cryo-TEM revealed elongated tube structures with a relatively uniform width but with a varying length. Through meticulous study, we concluded that the assembly process of these 3D DNA origami tubes is heavily dependent on the concentration of both mono- and divalent cations. In particular, nickel seems to act as a trigger for the formation of the tubular assemblies in liquid.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherRoyal Society of Chemistry (RSC)
dc.relation.ispartofseriesNanoscale
dc.rightsCC BY 3.0
dc.titleCreation of ordered 3D tubes out of DNA origami lattices
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202304262701
dc.contributor.laitosFysiikan laitosfi
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Physicsen
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineOrgaaninen kemiafi
dc.contributor.oppiaineNanoscience Centeren
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineOrganic Chemistryen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.format.pagerange7772-7780
dc.relation.issn2040-3364
dc.relation.numberinseries17
dc.relation.volume15
dc.type.versionpublishedVersion
dc.rights.copyright© The Royal Society of Chemistry 2023
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber330584
dc.relation.grantnumber330896
dc.subject.ysonanorakenteet
dc.subject.ysoDNA
dc.subject.ysotoiminnalliset materiaalit
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p25315
jyx.subject.urihttp://www.yso.fi/onto/yso/p7690
jyx.subject.urihttp://www.yso.fi/onto/yso/p21221
dc.rights.urlhttps://creativecommons.org/licenses/by/3.0/
dc.relation.doi10.1039/D2NR06001A
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderAcademy of Finlanden
dc.relation.funderAcademy of Finlanden
jyx.fundingprogramTutkijaliikkuvuusrahoitus, SAfi
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundingprogramResearcher mobility Funding, AoFen
jyx.fundingprogramAcademy Project, AoFen
jyx.fundinginformationFunding from the Jane and Aatos Erkko Foundation (J.J.T. and A.K./M.V.-R.) and the Academy of Finland (#330584 and #350797 J.J.T./#308992 A.K. and A.K.N./#330896 M.V.-R.) is gratefully acknowledged. The authors also acknowledge the provision of facilities and technical support by Aalto University at OtaNano – Nanomicroscopy Center (Aalto-NMC).
dc.type.okmA1


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