Liver fat biomarkers study from the 'Personalized diet intervention to treat fatty liver' project

Abstract

The study aimed at finding gut microbiota signatures and metabolite markers in plasma and feces related to high liver fat content in humans. Based on imaging, we divided study participants into low (<5%, LF, n=25) and high (>5%, HF, n=39) liver fat groups. Fecal (LF n=14, HF n=25) and plasma (LF n=11, HF n=7) metabolomes of subsets of participants were studied using liquid chromatography/high resolution mass spectrometry. The gut microbiota were analyzed using 16S rRNA gene sequencing. Additionally, blood clinical variables, body composition and diet were studied.

The list of metadata variables can be found below. They are categorized by the type of sample and/or analysis.

BLOOD CLINICAL VARIABLES & UNITS

alanine aminotransferase, ALT (U/L) aspartate aminotransferase, AST (U/L) gamma-glutamyltransferase, GGT(U/L) total cholesterol (mmol/L) high-density lipoprotein, HDL (mmol/L) low-density lipoprotein, LDL (mmol/L) triglycrides (mmol/L) glucose (mmol/L) insulin (iU/L) HOMA-IR

BODY COMPOSITION VARIABLES & UNITS

Height (cm) Weight (kg) body mass index, BMI (kg/m2) Waist circumference (cm) blood pressure (BP) systolic (mm Hg) blood pressure (BP) diastolic (mm Hg) Liver fat %

GUT MICROBIOTA COMPOSITION

fastq files of the sequences OUT counts per each sample relative abundance per each sample diversity measures (Shannon, Chao1, phylogenetic diversity, Bray Curtis distance)

METABOLITES

plasma metabolites & identification information fecal metabolites & identification information

DIETARY INTAKES & UNITS (from 3 different days & average from 3 days)

Energy (kJ) Energy (kcal) Protein (g) Carbohydrates (g) Fat (g) Alcohol Coffee (dl/3 days) * Fatty acids (g) Monosaturated Fatty acids (g) Polyunsaturated Fatty acids (g) Sucrose (g) Fiber, total (g) Vitamin C (mg) Thiamine (B1) (mg) Vitamin E (mg) Vitamin A (µg) Vitamin D (µg) Riboflavin (B2) (mg) Vitamin K (mg) Vitamin B6 (mg) Folate (B9) (µg) Sodium (mg) Magnesium (mg) Calcium (mg) Potassium (mg) Iron (mg) Iodine (µg) Phosphorus (mg) Selenium (µg) Zinc (mg) Fatty acids, total (g) Cholesterol (mg) Fatty acids, trans (g) Lactose (g) Fiber, total amount (g) Vitamin B12 (cobalamine) (µg) Salt (mg) Omega-6 Fatty acids (g) Omega-3 Fatty acids (g).