In-depth characterization of denitrifier communities across different soil ecosystems in the tundra
Pessi, I. S., Viitamäki, S., Virkkala, A.-M., Eronen-Rasimus, E., Delmont, T. O., Marushchak, M. E., Luoto, M., & Hultman, J. (2022). In-depth characterization of denitrifier communities across different soil ecosystems in the tundra. Environmental Microbiome, 17, Article 30. https://doi.org/10.1186/s40793-022-00424-2
Published inEnvironmental Microbiome
© The Author(s) 2022.
Background In contrast to earlier assumptions, there is now mounting evidence for the role of tundra soils as important sources of the greenhouse gas nitrous oxide (N2O). However, the microorganisms involved in the cycling of N2O in this system remain largely uncharacterized. Since tundra soils are variable sources and sinks of N2O, we aimed at investigating differences in community structure across different soil ecosystems in the tundra. Results We analysed 1.4 Tb of metagenomic data from soils in northern Finland covering a range of ecosystems from dry upland soils to water-logged fens and obtained 796 manually binned and curated metagenome-assembled genomes (MAGs). We then searched for MAGs harbouring genes involved in denitrification, an important process driving N2O emissions. Communities of potential denitrifiers were dominated by microorganisms with truncated denitrification pathways (i.e., lacking one or more denitrification genes) and differed across soil ecosystems. Upland soils showed a strong N2O sink potential and were dominated by members of the Alphaproteobacteria such as Bradyrhizobium and Reyranella. Fens, which had in general net-zero N2O fluxes, had a high abundance of poorly characterized taxa affiliated with the Chloroflexota lineage Ellin6529 and the Acidobacteriota subdivision Gp23. Conclusions By coupling an in-depth characterization of microbial communities with in situ measurements of N2O fluxes, our results suggest that the observed spatial patterns of N2O fluxes in the tundra are related to differences in the composition of denitrifier communities. ...
PublisherBioMed Central Ltd
Publication in research information system
MetadataShow full item record
Related funder(s)Academy of Finland
Funding program(s)Postdoctoral Researcher, AoF
Additional information about fundingThis work was funded by the Academy of Finland (Grants 314114 and 335354) and the University of Helsinki. SV was funded by the Microbiology and Biotechnology Doctoral Programme (MBDP). AMV was funded by the Academy of Finland (Grant 286950), the Otto Malm Foundation, and the Gordon and Betty Moore Foundation (Grant 8414). MEM was supported by the Academy of Finland (Grants 314630 and 317054). Open access funded by Helsinki University Library. ...
Showing items with similar title or keywords.
Saarenheimo, Jatta (University of Jyväskylä, 2015)
Emissions of atmospherically reactive gases nitrous acid and nitric oxide from Arctic permafrost peatlands Bhattarai, Hem Raj; Marushchak, Maija E; Ronkainen, Jussi; Lamprecht, Richard E; Siljanen, Henri M P; Martikainen, Pertti J; Biasi, Christina; Maljanen, Marja (IOP Publishing, 2022)Soils are important sources of nitric oxide (NO) and nitrous acid (HONO) in the atmosphere. These nitrogen (N)-containing gases play a crucial role in atmospheric chemistry and climate at different scales because of reactions ...
The ABCflux database : Arctic–boreal CO2 flux observations and ancillary information aggregated to monthly time steps across terrestrial ecosystems Virkkala, Anna-Maria; Natali, Susan M.; Rogers, Brendan M.; Watts, Jennifer D.; Savage, Kathleen; Connon, Sara June; Mauritz, Marguerite; Schuur, Edward A. G.; Peter, Darcy; Minions, Christina; Nojeim, Julia; Commane, Roisin; Emmerton, Craig A.; Goeckede, Mathias; Helbig, Manuel; Holl, David; Iwata, Hiroki; Kobayashi, Hideki; Kolari, Pasi; López-Blanco, Efrén; Marushchak, Maija E.; Mastepanov, Mikhail; Merbold, Lutz; Parmentier, Frans-Jan W.; Peichl, Matthias; Sachs, Torsten; Sonnentag, Oliver; Ueyama, Masahito; Voigt, Carolina; Aurela, Mika; Boike, Julia; Celis, Gerardo; Chae, Namyi; Christensen, Torben R.; Bret-Harte, M. Syndonia; Dengel, Sigrid; Dolman, Han; Edgar, Colin W.; Elberling, Bo; Euskirchen, Eugenie; Grelle, Achim; Hatakka, Juha; Humphreys, Elyn; Järveoja, Järvi; Kotani, Ayumi; Kutzbach, Lars; Laurila, Tuomas; Lohila, Annalea; Mammarella, Ivan; Matsuura, Yojiro; Meyer, Gesa; Nilsson, Mats B.; Oberbauer, Steven F.; Park, Sang-Jong; Petrov, Roman; Prokushkin, Anatoly S.; Schulze, Christopher; St. Louis, Vincent L.; Tuittila, Eeva-Stiina; Tuovinen, Juha-Pekka; Quinton, William; Varlagin, Andrej; Zona, Donatella; Zyryanov, Viacheslav I. (Copernicus GmbH, 2022)Past efforts to synthesize and quantify the magnitude and change in carbon dioxide (CO2) fluxes in terrestrial ecosystems across the rapidly warming Arctic–boreal zone (ABZ) have provided valuable information but were ...
Statistical upscaling of ecosystem CO2 fluxes across the terrestrial tundra and boreal domain : regional patterns and uncertainties Virkkala, Anna‐Maria; Aalto, Juha; Rogers, Brendan M.; Tagesson, Torbern; Treat, Claire C.; Natali, Susan M.; Watts, Jennifer D.; Potter, Stefano; Lehtonen, Aleksi; Mauritz, Marguerite; Schuur, Edward A.G.; Kochendorfer, John; Zona, Donatella; Oechel, Walter; Kobayashi, Hideki; Humphreys, Elyn; Goeckede, Mathias; Iwata, Hiroki; Lafleur, Peter M.; Euskirchen, Eugenie S.; Bokhorst, Stef; Marushchak, Maija; Martikainen, Pertti J.; Elberling, Bo; Voigt, Carolina; Biasi, Christina; Sonnentag, Oliver; Parmentier, Frans‐Jan W.; Ueyama, Masahito; Celis, Gerardo; St.Loius, Vincent L.; Emmerton, Craig A.; Peichl, Matthias; Chi, Jinshu; Järveoja, Järvi; Nilsson, Mats.B.; Oberbauer, Steven F.; Torn, Margaret S.; Park, Sang‐Jong; Dolman, Han; Mammarella, Ivan; Chae, Namyi; Poyatos, Rafael; López‐Blanco, Efrén; Røjle, Christensen Torben; Jung, Kwon Min; Sachs, Torsten; Holl, David; Luoto, Miska (Wiley, 2021)The regional variability in tundra and boreal carbon dioxide (CO2) fluxes can be high, complicating efforts to quantify sink‐source patterns across the entire region. Statistical models are increasingly used to predict ...
Nitrate removal microbiology in woodchip bioreactors : a case-study with full-scale bioreactors treating aquaculture effluents Aalto, Sanni L.; Suurnäkki, Suvi; von Ahnen, Mathis; Siljanen, Henri M. P.; Pedersen, Per Bovbjerg; Tiirola, Marja (Elsevier, 2020)Woodchip bioreactors are viable low-cost nitrate (NO3−) removal applications for treating agricultural and aquaculture discharges. The active microbial biofilms growing on woodchips are conducting nitrogen (N) removal, ...