Näytä suppeat kuvailutiedot

dc.contributor.authorLaine, Veronika N.
dc.contributor.authorSepers, Bernice
dc.contributor.authorLindner, Melanie
dc.contributor.authorGawehns, Fleur
dc.contributor.authorRuuskanen, Suvi
dc.contributor.authorOers, Kees
dc.date.accessioned2022-04-27T09:26:39Z
dc.date.available2022-04-27T09:26:39Z
dc.date.issued2023
dc.identifier.citationLaine, V. N., Sepers, B., Lindner, M., Gawehns, F., Ruuskanen, S., & Oers, K. (2023). An ecologist’s guide for studying DNA methylation variation in wild vertebrates. <i>Molecular Ecology Resources</i>, <i>23</i>(7), 1488-1508. <a href="https://doi.org/10.1111/1755-0998.13624" target="_blank">https://doi.org/10.1111/1755-0998.13624</a>
dc.identifier.otherCONVID_118907237
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/80756
dc.description.abstractThe field of molecular biology is advancing fast with new powerful technologies, sequencing methods and analysis software being developed constantly. Commonly used tools originally developed for research on humans and model species are now regularly used in ecological and evolutionary research. There is also a growing interest in the causes and consequences of epigenetic variation in natural populations. Studying ecological epigenetics is currently challenging, especially for vertebrate systems, because of the required technical expertise, complications with analyses and interpretation, and limitations in acquiring sufficiently high sample sizes. Importantly, neglecting the limitations of the experimental setup, technology and analyses may affect the reliability and reproducibility, and the extent to which unbiased conclusions can be drawn from these studies. Here, we provide a practical guide for researchers aiming to study DNA methylation variation in wild vertebrates. We review the technical aspects of epigenetic research, concentrating on DNA methylation using bisulfite sequencing, discuss the limitations and possible pitfalls and how to overcome them through rigid and reproducible data analysis. This review provides a solid foundation for the proper design of epigenetic studies, a clear roadmap on the best practices for correct data analysis and a realistic view on the limitations for studying ecological epigenetics in vertebrates. This review will help researchers studying the ecological and evolutionary implications of epigenetic variation in wild populations.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofseriesMolecular Ecology Resources
dc.rightsCC BY 4.0
dc.subject.otherepigenetics
dc.subject.otherDNA methylation
dc.subject.otherbisulfite sequencing
dc.subject.otherevolution
dc.subject.otherecology
dc.titleAn ecologist’s guide for studying DNA methylation variation in wild vertebrates
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202204272429
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_dcae04bc
dc.description.reviewstatuspeerReviewed
dc.format.pagerange1488-1508
dc.relation.issn1755-098X
dc.relation.numberinseries7
dc.relation.volume23
dc.type.versionacceptedVersion
dc.rights.copyright© 2022 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.
dc.rights.accesslevelopenAccessfi
dc.subject.ysoDNA-metylaatio
dc.subject.ysomuuntelu (biologia)
dc.subject.ysotutkimusmenetelmät
dc.subject.ysoevoluutioekologia
dc.subject.ysoepigenetiikka
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p38350
jyx.subject.urihttp://www.yso.fi/onto/yso/p8280
jyx.subject.urihttp://www.yso.fi/onto/yso/p415
jyx.subject.urihttp://www.yso.fi/onto/yso/p16528
jyx.subject.urihttp://www.yso.fi/onto/yso/p24631
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1111/1755-0998.13624
dc.type.okmA2


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