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dc.contributor.authorHiillos, Anna‐Lotta
dc.contributor.authorThonig, Anne
dc.contributor.authorKnott, Karelyn Emily
dc.date.accessioned2021-11-24T12:58:57Z
dc.date.available2021-11-24T12:58:57Z
dc.date.issued2021
dc.identifier.citationHiillos, A., Thonig, A., & Knott, K. E. (2021). Droplet digital PCR as a tool for investigating dynamics of cryptic symbionts. <i>Ecology and Evolution</i>, <i>11</i>(23), 17381-17396. <a href="https://doi.org/10.1002/ece3.8372" target="_blank">https://doi.org/10.1002/ece3.8372</a>
dc.identifier.otherCONVID_101986433
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/78778
dc.description.abstractInteractions among symbiotic organisms and their hosts are major drivers of ecological and evolutionary processes. Monitoring the infection patterns among natural populations and identifying factors affecting these interactions are critical for understanding symbiont–host relationships. However, many of these interactions remain understudied since the knowledge about the symbiont species is lacking, which hinders the development of appropriate tools. In this study, we developed a digital droplet PCR (ddPCR) assay based on apicomplexan COX1 gene to detect an undescribed agamococcidian symbiont. We show that the method gives precise and reproducible results and enables detecting cryptic symbionts in low target concentration. We further exemplify the assay's use to survey seasonally sampled natural host (Pygospio elegans) populations for symbiont infection dynamics. We found that symbiont prevalence differs spatially but does not show seasonal changes. Infection load differed between populations and was low in spring and significantly increased towards fall in all populations. We also found that the symbiont prevalence is affected by host length and population density. Larger hosts were more likely to be infected, and high host densities were found to have a lower probability of infection. The observed variations could be due to characteristics of both symbiont and host biology, especially the seasonal variation in encounter rates. Our findings show that the developed ddPCR assay is a robust tool for detecting undescribed symbionts that are otherwise difficult to quantify, enabling further insight into the impact cryptic symbionts have on their hosts.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofseriesEcology and Evolution
dc.rightsCC BY 4.0
dc.subject.otherapicomplexa
dc.subject.othercryptic symbiosis
dc.subject.otherdroplet digital PCR
dc.subject.otherinfection dynamics
dc.titleDroplet digital PCR as a tool for investigating dynamics of cryptic symbionts
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202111245786
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineEkologia ja evoluutiobiologiafi
dc.contributor.oppiaineEcology and Evolutionary Biologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.format.pagerange17381-17396
dc.relation.issn2045-7758
dc.relation.numberinseries23
dc.relation.volume11
dc.type.versionpublishedVersion
dc.rights.copyright© 2021 the Authors
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumbera6a412
dc.subject.ysoinfektiot
dc.subject.ysopopulaatiot
dc.subject.ysoloiset
dc.subject.ysoekosysteemit (ekologia)
dc.subject.ysoisäntäeläimet
dc.subject.ysoisäntälajit
dc.subject.ysosymbioosi
dc.subject.ysoevoluutiobiologia
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p7316
jyx.subject.urihttp://www.yso.fi/onto/yso/p5038
jyx.subject.urihttp://www.yso.fi/onto/yso/p4493
jyx.subject.urihttp://www.yso.fi/onto/yso/p4997
jyx.subject.urihttp://www.yso.fi/onto/yso/p24487
jyx.subject.urihttp://www.yso.fi/onto/yso/p23998
jyx.subject.urihttp://www.yso.fi/onto/yso/p8363
jyx.subject.urihttp://www.yso.fi/onto/yso/p21944
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.datasethttps://datadryad.org/stash/dataset/doi:10.5061/dryad.jwstqjq90
dc.relation.doi10.1002/ece3.8372
dc.relation.funderEmil Aaltonen Foundationen
dc.relation.funderEmil Aaltosen Säätiö srfi
jyx.fundingprogramFoundationen
jyx.fundingprogramSäätiöfi
jyx.fundinginformationEmil Aaltosen Säätiö, Grant/Award Number: a6a412; University of Jyväskylä Graduate School for Doctoral Studies
dc.type.okmA1


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