Mitochondrial bioenergetic pathways in blood leukocyte transcriptome decrease after intensive weight loss but are rescued following weight regain in female physique athletes
Sarin, H. V., Pirinen, E., Pietiläinen, K. H., Isola, V., Häkkinen, K., Perola, M., & Hulmi, J. J. (2021). Mitochondrial bioenergetic pathways in blood leukocyte transcriptome decrease after intensive weight loss but are rescued following weight regain in female physique athletes. FASEB Journal, 35(4), Article e21484. https://doi.org/10.1096/fj.202002029r
Published inFASEB Journal
DisciplineLiikuntafysiologiaValmennus- ja testausoppiExercise PhysiologyScience of Sport Coaching and Fitness Testing
© 2021 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology
Prolonged periods of energy deficit leading to weight loss induce metabolic adaptations resulting in reduced energy expenditure, but the mechanisms for energy conservation are incompletely understood. We examined 42 healthy athletic females (age 27.5 ± 4.0 years, body mass index 23.4 ± 1.7 kg/m2) who volunteered into either a group dieting for physique competition (n = 25) or a control group (n = 17). The diet group substantially reduced their energy intake and moderately increased exercise levels to induce loss of fat mass that was regained during a voluntary weight regain period. The control group maintained their typical lifestyle habits and body mass as instructed. From the diet group, fasting blood samples were drawn at baseline (PRE), after 4‐ to 5‐month weight loss (PRE‐MID), and after 4‐ to 5‐month weight regain (MID‐POST) as well as from the control group at similar intervals. Blood was analyzed to determine leukocyte transcriptome by RNA‐Sequencing and serum metabolome by nuclear magnetic resonance (NMR) platform. The intensive weight loss period induced several metabolic adaptations, including a prominent suppression of transcriptomic signature for mitochondrial OXPHOS and ribosome biogenesis. The upstream regulator analysis suggested that this reprogramming of cellular energy metabolism may be mediated via AMPK/PGC1‐α signaling and mTOR/eIF2 signaling‐dependent pathways. Our findings show for the first time that prolonged energy deprivation induced modulation of mitochondrial metabolism can be observed through minimally invasive measures of leukocyte transcriptome and serum metabolome at systemic level, suggesting that adaptation to energy deficit is broader in humans than previously thought. ...
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- Liikuntatieteiden tiedekunta 
Related funder(s)Academy of Finland
Funding program(s)Research post as Academy Research Fellow, AoF
Additional information about fundingAcademy of Finland (Suomen Akatemia), Grant/Award Number: 269517, 286359, 275922, 314383 and 266286; Academy of Finland, Centre of Excellence in Research on Mitochondria, Metabolism and Disease, Grant/Award Number: 272376; Novo Nordisk Foundation Center for Basic Metabolic Research, Grant/Award Number: NNF16OC0020866, NNF17OC0027232 and NNF10OC1013354; Juho Vainion Säätiö; Orionin Tutkimussäätiö (Orion Research Foundation); The European Union's FP7 programme, Grant/Award Number: HZ2020 633589 (Ageing with Elegans); Yrjö Jahnsson Foundation; Suomen Lääketieteen Säätiö (Finnish Medical Foundation); Finnish Diabetes Research Foundation; Signe ja Ane Gyllenbergin Säätiö (Signe and Ane Gyllenberg Foundation); Sigrid Juselius Foundation; Helsinki University Hospital Research Funds; Government Research Funds; Helsingin Yliopisto ja Jyväskylän yliopisto (University of Helsinki and Jyväskylä) ...
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