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dc.contributor.advisorSievänen, Elina
dc.contributor.authorAhonen, Jari
dc.date.accessioned2020-02-06T10:01:36Z
dc.date.available2020-02-06T10:01:36Z
dc.date.issued2020
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/67764
dc.description.abstractThis thesis begins with a literature review that presents the basics of the fluorescence phenomenon and fluorescence microscopy. The literature on cellular cholesterol composition and cholesterol transport mechanisms is extensively studied. Thereafter, the fluorescent steroid-based molecules designed to identify cholesterol and cholesteryl esters suitable for use in living cells are covered. The literature review ends an overview of other applications for the use of fluorescent probes, for example in cell biology. The aim of the experimental part was to optimize the synthesis pathway for producing fluorescent biocompatible bile acid-based molecules designed for the detection of cholesterol and cholesteryl esters. Preliminary cholesterol-binding assays were also performed for the prepared cholesterol probes by NMR titration, diffusion NMR-experiments, and mass spectrometry.en
dc.format.extent113
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.subject.othercholesterol-probes
dc.subject.othersteroid-derivatived
dc.titleFluorescence in the study of steroid metabolism
dc.identifier.urnURN:NBN:fi:jyu-202002062010
dc.type.ontasotLisensiaatintyöfi
dc.type.ontasotLicentiate thesisen
dc.contributor.tiedekuntaMatemaattis-luonnontieteellinen tiedekuntafi
dc.contributor.tiedekuntaFaculty of Sciencesen
dc.contributor.laitosKemian laitosfi
dc.contributor.laitosDepartment of Chemistryen
dc.contributor.yliopistoJyväskylän yliopistofi
dc.contributor.yliopistoUniversity of Jyväskyläen
dc.contributor.oppiaineOrgaaninen kemiafi
dc.contributor.oppiaineOrganic Chemistryen
dc.rights.copyrightJulkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.fi
dc.rights.copyrightThis publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.en
dc.contributor.oppiainekoodi4035
dc.subject.ysofluoresenssi
dc.subject.ysoaineenvaihdunta
dc.subject.ysofluorescence
dc.subject.ysometabolism
dc.format.contentfulltext
dc.type.okmG3


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