University of Jyväskylä | JYX Digital Repository

  • English  | Give feedback |
    • suomi
    • English
 
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.
View Item 
  • JYX
  • Opinnäytteet
  • Lisensiaatintyöt
  • View Item
JYX > Opinnäytteet > Lisensiaatintyöt > View Item

Fluorescence in the study of steroid metabolism

Thumbnail
View/Open
10.Mb

Downloads:  
Show download detailsHide download details  
Authors
Ahonen, Jari
Date
2020
Discipline
Orgaaninen kemiaOrganic Chemistry
Copyright
This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.

 
This thesis begins with a literature review that presents the basics of the fluorescence phenomenon and fluorescence microscopy. The literature on cellular cholesterol composition and cholesterol transport mechanisms is extensively studied. Thereafter, the fluorescent steroid-based molecules designed to identify cholesterol and cholesteryl esters suitable for use in living cells are covered. The literature review ends an overview of other applications for the use of fluorescent probes, for example in cell biology. The aim of the experimental part was to optimize the synthesis pathway for producing fluorescent biocompatible bile acid-based molecules designed for the detection of cholesterol and cholesteryl esters. Preliminary cholesterol-binding assays were also performed for the prepared cholesterol probes by NMR titration, diffusion NMR-experiments, and mass spectrometry.
Keywords
cholesterol-probes steroid-derivatived fluoresenssi aineenvaihdunta fluorescence metabolism
URI

http://urn.fi/URN:NBN:fi:jyu-202002062010

Metadata
Show full item record
Collections
  • Lisensiaatintyöt [490]

Related items

Showing items with similar title or keywords.

  • 160218/MLH: TUPLA; VIRHEELL. In Situ Formation of Steroidal Supramolecular Gels Designed for Drug Release 

    Svobodova, Hana; Kolehmainen, Erkki (MDPI, 2013)
    In this work, a steroidal gelator containing an imine bond was synthesized, and its gelation behavior as well as a sensitivity of its gels towards acids was investigated. It was shown that the gels were acid-responsive, ...
  • Steroidal derivatives of nitrogen containing compounds as potential gelators 

    Bunzen, Hana (University of Jyväskylä, 2013)
  • Spectroscopic studies of semiconducting single-walled carbon nanotubes 

    Siitonen, Anni (University of Jyväskylä, 2010)
    The unique nature of optical properties of single-walled carbon nanotubes (SWCNT), together with their promising potential applications, have created enormous interest towards the photophysics of SWCNT. Many aspects ...
  • On dynamics of parvoviral replication protein NS1 

    Niskanen, Einari (University of Jyväskylä, 2010)
     
  • Synthesis and structural characterization of new transition metal complexes of a highly luminescent amino-terpyridine ligand 

    Nath Ghosh, Biswa; Puttreddy, Rakesh; Rissanen, Kari (Elsevier Ltd, 2020)
    The synthesis, NMR and UV-Vis spectroscopy measurements and X-ray diffraction analysis of four new metal complexes of the amino terpyridine ligand 4́-[4-(4-aminophenyl)phenyl]-2,2́:6́,2́́-terpyridine L, namely [FeL2](ClO4)2 ...
  • Browse materials
  • Browse materials
  • Articles
  • Conferences and seminars
  • Electronic books
  • Historical maps
  • Journals
  • Tunes and musical notes
  • Photographs
  • Presentations and posters
  • Publication series
  • Research reports
  • Research data
  • Study materials
  • Theses

Browse

All of JYXCollection listBy Issue DateAuthorsSubjectsPublished inDepartmentDiscipline

My Account

Login

Statistics

View Usage Statistics
  • How to publish in JYX?
  • Self-archiving
  • Publish Your Thesis Online
  • Publishing Your Dissertation
  • Publication services

Open Science at the JYU
 
Data Protection Description

Accessibility Statement

Unless otherwise specified, publicly available JYX metadata (excluding abstracts) may be freely reused under the CC0 waiver.
Open Science Centre