Fluorescence in the study of steroid metabolism
This thesis begins with a literature review that presents the basics of the fluorescence phenomenon and fluorescence microscopy. The literature on cellular cholesterol composition and cholesterol transport mechanisms is extensively studied. Thereafter, the fluorescent steroid-based molecules designed to identify cholesterol and cholesteryl esters suitable for use in living cells are covered. The literature review ends an overview of other applications for the use of fluorescent probes, for example in cell biology.
The aim of the experimental part was to optimize the synthesis pathway for producing fluorescent biocompatible bile acid-based molecules designed for the detection of cholesterol and cholesteryl esters. Preliminary cholesterol-binding assays were also performed for the prepared cholesterol probes by NMR titration, diffusion NMR-experiments, and mass spectrometry.
Keywords
Metadata
Show full item recordCollections
- Lisensiaatintyöt [514]
License
Related items
Showing items with similar title or keywords.
-
Designing fluorescent probes for the study of intracellular cholesterol metabolism
Hintikka, Jukka (2019)Cholesterol is abundantly present in animal cells. It comprises a major portion of the lipids in mammalian cell membranes and is required for the synthesis of steroid hormones, lipoproteins and bile acids. Many characteristics ... -
Steroidal derivatives of nitrogen containing compounds as potential gelators
Bunzen, Hana (University of Jyväskylä, 2013) -
Eating behavior and cardiometabolic health : associations of eating habits with glucose and lipid metabolism in menopausal women
Kansanen, Mari (2021)Background: Cardiovascular diseases (CVD) are the primary cause of death in women in developed countries. CVD risk increases with age and further accentuates after the onset of menopause in women. This finding suggests ... -
Prolonged sleep restriction induces changes in pathways involved in cholesterol metabolism and inflammatory responses
Aho, Vilma; Ollila, Hanna M.; Kronholm, Erkki; Bondia-Pons, Isabel; Soininen, Pasi; Kangas, Antti J.; Hilvo, Mika; Seppälä, Ilkka; Kettunen, Johannes; Oikonen, Mervi; Raitoharju, Emma; Hyötyläinen, Tuulia; Kähönen, Mika; Viikari, Jorma S.A.; Härmä, Mikko; Sallinen, Mikael; Olkkonen, Vesa M.; Alenius, Harri; Jauhiainen, Matti; Paunio, Tiina; Lehtimäki, Terho; Salomaa, Veikko; Orešič, Matej; Raitakari, Olli T.; Ala-Korpela, Mika; Porkka-Heiskanen, Tarja (Nature Publishing Group, 2016)Sleep loss and insufficient sleep are risk factors for cardiometabolic diseases, but data on how insufficient sleep contributes to these diseases are scarce. These questions were addressed using two approaches: an ...