Show simple item record

dc.contributor.authorTurkki, Paula
dc.contributor.authorLaajala, Mira
dc.contributor.authorStark, Marie
dc.contributor.authorVandesande, Helena
dc.contributor.authorSallinen-Dal Maso, Heidi
dc.contributor.authorShroff, Sailee
dc.contributor.authorSävneby, Anna
dc.contributor.authorGalitska, Ganna
dc.contributor.authorLindberg, A. Michael
dc.contributor.authorMarjomäki, Varpu
dc.date.accessioned2019-08-27T08:42:32Z
dc.date.available2019-08-27T08:42:32Z
dc.date.issued2019
dc.identifier.citationTurkki, P., Laajala, M., Stark, M., Vandesande, H., Sallinen-Dal Maso, H., Shroff, S., Sävneby, A., Galitska, G., Lindberg, A. M., & Marjomäki, V. (2019). Slow infection due to lowering amount of intact versus empty particles is a characteristic feature of Coxsackievirus B5, dictated by the structural proteins. <i>Journal of Virology</i>, <i>93</i>(20), e01130-19. <a href="https://doi.org/10.1128/JVI.01130-19" target="_blank">https://doi.org/10.1128/JVI.01130-19</a>
dc.identifier.otherCONVID_32498145
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/65321
dc.description.abstractEnterovirus B species typically cause a rapid cytolytic infection leading to efficient release of progeny viruses. However, they are also capable of persistent infections in tissues, which are suggested to contribute to severe chronic states such as myocardial inflammation and type 1 diabetes. In order to understand the factors contributing to differential infection strategies, we constructed a chimera by combining the capsid proteins from a fast cytolysis causing echovirus 1 (EV1) with non-structural proteins from Coxsackievirus B5 (CVB5) showing persistent infection in RD cells. The results showed that the chimera behaved similar to the parental EV1 leading to efficient cytolysis in both permissive A549 and semi-permissive RD cells. In contrast to EV1 and chimera, CVB5 replicated slower in permissive cells and showed persistent infection in semi-permissive cells. However, there was no difference in the efficiency of uptake of CVB5 in A549 or RD cells in comparison to the chimera or EV1. CVB5 virus batches constantly contained significant amounts of empty capsids, also in comparison to its close relative CVB3. During successive passaging of batch containing only intact CVB5, increasing amounts of empty and decreasing amounts of infective capsids were produced. Our results demonstrate that the increased amounts of empty particles and lowering amounts of infective particles is dictated by the CVB5 structural proteins leading to slowing down the infection between passages. Furthermore, the key factor for persistent infection is the low amount of infective particles produced, not the high number of empty particles accumulating.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofseriesJournal of Virology
dc.rightsIn Copyright
dc.titleSlow infection due to lowering amount of intact versus empty particles is a characteristic feature of Coxsackievirus B5, dictated by the structural proteins
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201908273919
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centeren
dc.contributor.oppiaineCell and Molecular Biologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.description.reviewstatuspeerReviewed
dc.format.pagerangee01130-19
dc.relation.issn0022-538X
dc.relation.numberinseries20
dc.relation.volume93
dc.type.versionacceptedVersion
dc.rights.copyright© 2019 American Society for Microbiology
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber
dc.subject.ysoenterovirukset
dc.subject.ysokapsidi
dc.subject.ysoproteiinit
dc.subject.ysoinfektiot
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p20689
jyx.subject.urihttp://www.yso.fi/onto/yso/p28020
jyx.subject.urihttp://www.yso.fi/onto/yso/p4332
jyx.subject.urihttp://www.yso.fi/onto/yso/p7316
dc.rights.urlhttp://rightsstatements.org/page/InC/1.0/?language=en
dc.relation.doi10.1128/JVI.01130-19
dc.relation.funderJane ja Aatos Erkon säätiöfi
dc.relation.funderJane and Aatos Erkko Foundationen
jyx.fundingprogramSäätiöfi
jyx.fundingprogramFoundationen
jyx.fundinginformationThis work was supported by the Jane and Aatos Erkko Foundation (V.M.).


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

In Copyright
Except where otherwise noted, this item's license is described as In Copyright