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dc.contributor.advisorSundberg, Lotta-Riina
dc.contributor.advisorHoikkala, Ville
dc.contributor.authorSmith, Katie
dc.date.accessioned2019-07-30T05:58:54Z
dc.date.available2019-07-30T05:58:54Z
dc.date.issued2019
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/65152
dc.description.abstractThe freshwater bacterium Flavobacterium columnare is known to exhibit different morphologies when cultured in different media with bacteriophage. This could influence the type of phage-resistance mechanisms the bacteria use, such as CRISPR-Cas system or alterations which reduce bacteriophage adsorption. In this study the different morphologies which arise in different media when treated with bacteriophage was investigated to identify their favoured phage-resistance mechanism. The bacteria were screened for acquisition of new CRISPR spaces and tested for changes in phage adsorption. Results show that the growth medium itself influences the bacterial colony morphology, likely due to the difference in nutrients available. When exposed to bacteriophage, a small proportion of bacteria in HTC medium acquired new CRISPR spacers, however, the preferred phage-resistance mechanism appears to be something other than CRISPR-Cas overall, in SHIEH and HTC. Improved understanding of bacterial resistance mechanisms is useful towards the development of bacteriophage therapies.en
dc.format.extent47
dc.language.isoen
dc.subject.otherFlavobacterium columnare
dc.subject.othercolony morphology
dc.subject.otherCRISPR-Cas
dc.subject.otherPhage resistance
dc.titleThe effects of growth medium on Flavobacterium columnare colony morphology and its phage resistance mechanisms
dc.identifier.urnURN:NBN:fi:jyu-201907303713
dc.type.ontasotMaster’s thesisen
dc.type.ontasotPro gradu -tutkielmafi
dc.contributor.tiedekuntaMatemaattis-luonnontieteellinen tiedekuntafi
dc.contributor.tiedekuntaFaculty of Sciencesen
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.yliopistoJyväskylän yliopistofi
dc.contributor.yliopistoUniversity of Jyväskyläen
dc.contributor.oppiaineAkvaattiset tieteetfi
dc.contributor.oppiaineAquatic sciencesen
dc.rights.copyrightJulkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.fi
dc.rights.copyrightThis publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.en
dc.contributor.oppiainekoodi4012
dc.subject.ysobakteerit
dc.subject.ysobakteriofagit
dc.subject.ysobacteria
dc.subject.ysobacteriophages


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