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dc.contributor.authorWoitowich, Nicole C.
dc.contributor.authorHalavaty, Andrei S.
dc.contributor.authorWaltz, Patricia
dc.contributor.authorKupitz, Christopher
dc.contributor.authorValera, Joseph
dc.contributor.authorTracy, Gregory
dc.contributor.authorGallagher, Kevin D.
dc.contributor.authorClaesson, Elin
dc.contributor.authorNakane, Takanori
dc.contributor.authorPandey, Suraj
dc.contributor.authorNelson, Garrett
dc.contributor.authorTanaka, Rie
dc.contributor.authorNango, Eriko
dc.contributor.authorMizohata, Eiichi
dc.contributor.authorOwada, Shigeki
dc.contributor.authorTono, Kensure
dc.contributor.authorJoti, Yasumasa
dc.contributor.authorNugent, Angela C.
dc.contributor.authorPatel, Hardik
dc.contributor.authorMapara, Ayesha
dc.contributor.authorHopkins, James
dc.contributor.authorDuong, Phu
dc.contributor.authorBizhga, Dorina
dc.contributor.authorKovaleva, Svetlana E.
dc.contributor.authorPeter, Rachael St.
dc.contributor.authorHernandez, Cynthia N.
dc.contributor.authorOzarowski, Wesley B.
dc.contributor.authorRoy-Chowdhuri, Shatabdi
dc.contributor.authorYang, Jay-How
dc.contributor.authorEdlund, Petra
dc.contributor.authorTakala, Heikki
dc.contributor.authorIhalainen, Janne
dc.contributor.authorBrayshaw, Jennifer
dc.contributor.authorNorwood, Tyler
dc.contributor.authorPoudyal, Ishwor
dc.contributor.authorFromme, Petra
dc.contributor.authorSpence, John C. H.
dc.contributor.authorMoffat, Keith
dc.contributor.authorWestenhoff, Sebastian
dc.contributor.authorSchmidt, Marius
dc.contributor.authorStojković, Emina A.
dc.date.accessioned2018-09-26T07:22:16Z
dc.date.available2018-09-26T07:22:16Z
dc.date.issued2018fi
dc.identifier.citationWoitowich, N. C., Halavaty, A. S., Waltz, P., Kupitz, C., Valera, J., Tracy, G., . . . Stojković, E. A. (2018). Structural basis for light control of cell development revealed by crystal structures of a myxobacterial phytochrome. <em>IUCrJ</em>, 5 (5), 619-634. <a href="https://doi.org/10.1107/S2052252518010631">doi:10.1107/S2052252518010631</a>fi
dc.identifier.otherTUTKAID_78946
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/59673
dc.description.abstractPhytochromes are red-light photoreceptors that were first characterized in plants, with homologs in photosynthetic and non-photosynthetic bacteria known as bacteriophytochromes (BphPs). Upon absorption of light, BphPs interconvert between two states denoted Pr and Pfr with distinct absorption spectra in the red and far-red. They have recently been engineered as enzymatic photoswitches for fluorescent-marker applications in non-invasive tissue imaging of mammals. This article presents cryo- and room-temperature crystal structures of the unusual phytochrome from the non-photosynthetic myxo­bacterium Stigmatella aurantiaca (SaBphP1) and reveals its role in the fruiting-body formation of this photomorphogenic bacterium. SaBphP1 lacks a conserved histidine (His) in the chromophore-binding domain that stabilizes the Pr state in the classical BphPs. Instead it contains a threonine (Thr), a feature that is restricted to several myxobacterial phytochromes and is not evolutionarily understood. SaBphP1 structures of the chromophore binding domain (CBD) and the complete photosensory core module (PCM) in wild-type and Thr-to-His mutant forms reveal details of the molecular mechanism of the Pr/Pfr transition associated with the physiological response of this myxobacterium to red light. Specifically, key structural differences in the CBD and PCM between the wild-type and the Thr-to-His mutant involve essential chromophore contacts with proximal amino acids, and point to how the photosignal is transduced through the rest of the protein, impacting the essential enzymatic activity in the photomorphogenic response of this myxobacterium.fi
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherInternational Union of Crystallography
dc.relation.ispartofseriesIUCrJ
dc.rightsCC BY 2.0
dc.subject.otherfotobiologiafi
dc.subject.otheryhteyttäminenfi
dc.subject.otherbakteeritfi
dc.subject.otherproteiinitfi
dc.subject.otherphytochromesfi
dc.subject.otherphotoreceptorsfi
dc.subject.otherphotosynthetic bacteriafi
dc.subject.othermyxobacteriafi
dc.subject.otherabsorption spectrafi
dc.titleStructural basis for light control of cell development revealed by crystal structures of a myxobacterial phytochromefi
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201809254233
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosThe Department of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologia
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2018-09-25T09:15:09Z
dc.description.reviewstatuspeerReviewed
dc.format.pagerange619-634
dc.relation.issn2052-2525
dc.relation.numberinseries5
dc.relation.volume5
dc.type.versionpublishedVersion
dc.rights.copyright© 2018 the Authors
dc.rights.accesslevelopenAccessfi
dc.format.contentfulltext
dc.rights.urlhttps://creativecommons.org/licenses/by/2.0/uk/legalcode
dc.relation.doi10.1107/S2052252518010631


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