Identification and structural characterization of LytU, a unique peptidoglycan endopeptidase from the lysostaphin family
Raulinaitis, V., Tossavainen, H., Aitio, O., Juuti, J. T., Hiramatsu, K., Kontinen, V., & Permi, P. (2017). Identification and structural characterization of LytU, a unique peptidoglycan endopeptidase from the lysostaphin family. Scientific Reports, 7, Article 6020. https://doi.org/10.1038/s41598-017-06135-w
Julkaistu sarjassa
Scientific ReportsTekijät
Päivämäärä
2017Oppiaine
Solu- ja molekyylibiologiaOrgaaninen kemiaNanoscience CenterCell and Molecular BiologyOrganic ChemistryNanoscience CenterTekijänoikeudet
© The Author(s) 2017. This is an open access article distributed under the terms of the Creative Commons Attribution License.
We introduce LytU, a short member of the lysostaphin family of zinc-dependent pentaglycine endopeptidases. It is a potential antimicrobial agent for S. aureus infections and its gene transcription is highly upregulated upon antibiotic treatments along with other genes involved in cell wall synthesis. We found this enzyme to be responsible for the opening of the cell wall peptidoglycan layer during cell divisions in S. aureus. LytU is anchored in the plasma membrane with the active part residing in the periplasmic space. It has a unique Ile/Lys insertion at position 151 that resides in the catalytic site-neighbouring loop and is vital for the enzymatic activity but not affecting the overall structure common to the lysostaphin family. Purified LytU lyses S. aureus cells and cleaves pentaglycine, a reaction conveniently monitored by NMR spectroscopy. Substituting the cofactor zinc ion with a copper or cobalt ion remarkably increases the rate of pentaglycine cleavage. NMR and isothermal titration calorimetry further reveal that, uniquely for its family, LytU is able to bind a second zinc ion which is coordinated by catalytic histidines and is therefore inhibitory. The pH-dependence and high affinity of binding carry further physiological implications.
...
Julkaisija
Nature Publishing GroupISSN Hae Julkaisufoorumista
2045-2322Asiasanat
Julkaisu tutkimustietojärjestelmässä
https://converis.jyu.fi/converis/portal/detail/Publication/27136429
Metadata
Näytä kaikki kuvailutiedotKokoelmat
Lisenssi
Ellei muuten mainita, aineiston lisenssi on © The Author(s) 2017. This is an open access article distributed under the terms of the Creative Commons Attribution License.
Samankaltainen aineisto
Näytetään aineistoja, joilla on samankaltainen nimeke tai asiasanat.
-
Structural and Functional Insights into Lysostaphin-Substrate Interaction
Tossavainen, Helena; Raulinaitis, Vytas; Kauppinen, Linda; Pentikäinen, Ulla; Maaheimo, Hannu; Permi, Perttu (Frontiers Research Foundation, 2018)Lysostaphin from Staphylococcus simulans and its family enzymes rapidly acquire prominence as the next generation agents in treatment of S. aureus infections. The specificity of lysostaphin is promoted by its C-terminal ... -
Synthesis, characterization, crystal structures and biological screening of 4-amino quinazoline sulfonamide derivatives
Kumar, A. Sunil; Kudva, Jyothi; Lahtinen, Manu; Peuronen, Anssi; Sadashiva, Rajitha; Naral, Damodara (Elsevier BV, 2019)Three quinazolin-4-ylamino derivatives containing phenylbenzenesulfonamides (7a-7c) were synthesized by reacting (E)-N'-(2-cyanophenyl)-N,N-dimethyl formamidine (6) with different 4- amino-N-(phenyl)benzenesulfonamides ... -
Determining the substrate specificity of two lysostaphin enzyme family members using NMR spectroscopy
Jartti, Minne (2020)Yksi merkittävimmistä ihmisen patogeeneista on Staphylococcus aureus-bakteeri. Se aiheuttaa erityyppisiä vakavia infektioita kuten verenmyrkytystä ja sydämen sisäkalvon tulehdusta, sekä lievempiä ihotulehduksia ja ... -
Synthesis and X-ray Structure Analysis of the Polymeric [Ag2(4-Amino-4H-1,2,4-triazole)2(NO3)]n(NO3)n Adduct : Anticancer, and Antimicrobial Applications
El-Naggar, Mostafa A.; Al-Rasheed, Hessa H.; AL-khamis, Sarah A.; El-Faham, Ayman; Abu-Youssef, Morsy A. M.; Haukka, Matti; Barakat, Assem; Sharaf, Mona M.; Soliman, Saied M. (MDPI AG, 2023)A new Ag(I) adduct was synthesized by the reaction of 4-amino-4H-1,2,4-triazole (L) with AgNO3. Its chemical structure was approved to be [Ag2(L)2(NO3)]n(NO3)n utilizing elemental analysis, FTIR spectra, and single crystal ... -
Synthesis, X-ray Structure, Antimicrobial and Anticancer Activity of a Novel [Ag(ethyl-3-quinolate)2(citrate)] Complex
Altowyan, Mezna Saleh; El-Naggar, Mostafa A.; Abu-Youssef, Morsy A. M.; Soliman, Saied M.; Haukka, Matti; Barakat, Assem; Badr, Ahmed M. A. (MDPI AG, 2022)A novel Ag(I) citrate complex with ethyl-3-quinolate (Et3qu) was synthesized. Its structure was confirmed using X-ray single crystal to be [Ag(Et3qu)2(citrate)]. It crystallized in the Triclinic crystal system and P-1 space ...
Ellei toisin mainittu, julkisesti saatavilla olevia JYX-metatietoja (poislukien tiivistelmät) saa vapaasti uudelleenkäyttää CC0-lisenssillä.