dc.contributor.author | Ahlstrand, Tuuli | |
dc.contributor.author | Tuominen, Heidi | |
dc.contributor.author | Beklen, Arzu | |
dc.contributor.author | Torittu, Annamari | |
dc.contributor.author | Oscarsson, Jan | |
dc.contributor.author | Sormunen, Raija | |
dc.contributor.author | Pöllänen, Marja T. | |
dc.contributor.author | Permi, Perttu | |
dc.contributor.author | Ihalina, Riikka | |
dc.date.accessioned | 2017-03-08T06:37:00Z | |
dc.date.available | 2017-03-08T06:37:00Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Ahlstrand, T., Tuominen, H., Beklen, A., Torittu, A., Oscarsson, J., Sormunen, R., Pöllänen, M. T., Permi, P., & Ihalina, R. (2017). A novel intrinsically disordered outer membrane lipoprotein of Aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8. <i>Virulence</i>, <i>8</i>(2), 115-134. <a href="https://doi.org/10.1080/21505594.2016.1216294" target="_blank">https://doi.org/10.1080/21505594.2016.1216294</a> | |
dc.identifier.other | CONVID_26134644 | |
dc.identifier.other | TUTKAID_70750 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/53206 | |
dc.description.abstract | Intrinsically disordered proteins (IDPs) do not have a well-defined and stable 3-dimensional fold.
Some IDPs can function as either transient or permanent binders of other proteins and may interact
with an array of ligands by adopting different conformations. A novel outer membrane lipoprotein,
bacterial interleukin receptor I (BilRI) of the opportunistic oral pathogen Aggregatibacter
actinomycetemcomitans binds a key gatekeeper proinflammatory cytokine interleukin (IL)-1b.
Because the amino acid sequence of the novel lipoprotein resembles that of fibrinogen binder A of
Haemophilus ducreyi, BilRI could have the potential to bind other proteins, such as host matrix
proteins. However, from the tested host matrix proteins, BilRI interacted with neither collagen nor
fibrinogen. Instead, the recombinant non-lipidated BilRI, which was intrinsically disordered, bound
various pro/anti-inflammatory cytokines, such as IL-8, tumor necrosis factor (TNF)-a, interferon (IFN)-
g and IL-10. Moreover, BilRI played a role in the in vitro sensing of IL-1b and IL-8 because low
concentrations of cytokines did not decrease the amount of extracellular DNA in the matrix of bilRI¡
mutant biofilm as they did in the matrix of wild-type biofilm when the biofilms were exposed to
recombinant cytokines for 22 hours. BilRI played a role in the internalization of IL-1b in the gingival
model system but did not affect either IL-8 or IL-6 uptake. However, bilRI deletion did not entirely
prevent IL-1b internalization, and the binding of cytokines to BilRI was relatively weak. Thus, BilRI
might sequester cytokines on the surface of A. actinomycetemcomitans to facilitate the
internalization process in low local cytokine concentrations. | |
dc.language.iso | eng | |
dc.publisher | Landes Bioscience | |
dc.relation.ispartofseries | Virulence | |
dc.subject.other | aggregatibacter actinomycetemcomitans | |
dc.subject.other | bacterial cytokine receptors | |
dc.subject.other | biofilm matrix compositions | |
dc.subject.other | intrinsically disordered proteins | |
dc.subject.other | outer membrane lipoproteins | |
dc.title | A novel intrinsically disordered outer membrane lipoprotein of Aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8 | |
dc.type | article | |
dc.identifier.urn | URN:NBN:fi:jyu-201703061582 | |
dc.contributor.laitos | Bio- ja ympäristötieteiden laitos | fi |
dc.contributor.laitos | Kemian laitos | fi |
dc.contributor.laitos | Department of Biological and Environmental Science | en |
dc.contributor.laitos | Department of Chemistry | en |
dc.contributor.oppiaine | Solu- ja molekyylibiologia | fi |
dc.contributor.oppiaine | Orgaaninen kemia | fi |
dc.contributor.oppiaine | Nanoscience Center | fi |
dc.contributor.oppiaine | Cell and Molecular Biology | en |
dc.contributor.oppiaine | Organic Chemistry | en |
dc.contributor.oppiaine | Nanoscience Center | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.date.updated | 2017-03-06T10:15:06Z | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.format.pagerange | 115-134 | |
dc.relation.issn | 2150-5594 | |
dc.relation.numberinseries | 2 | |
dc.relation.volume | 8 | |
dc.type.version | publishedVersion | |
dc.rights.copyright | © 2017 the Authors. Published with license by Taylor & Francis.
This is an open access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License. | |
dc.rights.accesslevel | openAccess | fi |
dc.rights.url | https://creativecommons.org/licenses/by-nc/3.0/ | |
dc.relation.doi | 10.1080/21505594.2016.1216294 | |
dc.type.okm | A1 | |