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dc.contributor.authorKarvinen, Sira
dc.contributor.authorSilvennoinen, Mika
dc.contributor.authorVainio, Petra
dc.contributor.authorSistonen, Lea
dc.contributor.authorKoch, Lauren G.
dc.contributor.authorBritton, Steven L.
dc.contributor.authorKainulainen, Heikki
dc.date.accessioned2016-04-26T04:43:19Z
dc.date.available2017-03-31T21:45:06Z
dc.date.issued2016
dc.identifier.citationKarvinen, S., Silvennoinen, M., Vainio, P., Sistonen, L., Koch, L. G., Britton, S. L., & Kainulainen, H. (2016). Effects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins. <i>Experimental Gerontology</i>, <i>79</i>, 46-54. <a href="https://doi.org/10.1016/j.exger.2016.03.015" target="_blank">https://doi.org/10.1016/j.exger.2016.03.015</a>
dc.identifier.otherCONVID_25629110
dc.identifier.otherTUTKAID_69621
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/49551
dc.description.abstractAim Sirtuins are proteins that connect energy metabolism, oxidative stress and aging. Expression of heat shock proteins (Hsps) is regulated by heat shock factors (HSFs) in response to various environmental and physiological stresses, such as oxidative stress. Oxidative stress accumulates during aging which makes cells more prone to DNA damage. Although many experimental animal models have been designed to study the effects of knockdown or overexpression of sirtuins, HSFs and Hsps, little is known about how aging per se affects their expression. Here we study the impact of intrinsic aerobic capacity, aging and voluntary exercise on the levels of sirtuins, HSFs and Hsps in skeletal muscle. Methods We studied the protein levels of sirtuins (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT7), HSF1, HSF2, Hsp10, Hsp27 and Hsp70 before and after one-year of voluntary running intervention of rat strains selectively bred for intrinsic aerobic exercise capacity; high capacity runners (HCR) and low capacity runners (LCR) differ by more than 30% for median lifespan. This setup enabled us to discern the effects of inborn aerobic capacity, aging and exercise activity on the protein levels of sirtuins, HSFs and Hsps in skeletal muscle. Results Our results revealed that the longer lived HCR rats had higher SIRT3, HSF1 and HSF2 contents in skeletal muscle (gastrocnemius, p < 0.05) than LCRs. Neither aging nor voluntary running had a significant effect on the studied sirtuin proteins. Aging significantly increased the protein levels of HSF1, HSF2 and Hsp27 (p < 0.05). Conclusion Our finding of elevated SIRT3 levels in HCR rats is in line with previous studies; SIRT3 in general is linked to elevated fatty acid oxidation and oxidative phosphorylation, which previously have been associated with metabolic profile of HCRs. HSF1, HSF2 and Hsp27 levels increased with aging, showing that aged muscles responded to aging-related stress. Our study shows for the first time that SIRT3 protein level is linked to high inborn aerobic capacity, and may be directly interconnected to longevity.
dc.language.isoeng
dc.publisherElsevier Inc.
dc.relation.ispartofseriesExperimental Gerontology
dc.subject.otheraging
dc.subject.otherphysical activity
dc.subject.othersirtuin
dc.subject.otherskeletal muscle
dc.titleEffects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201604212285
dc.contributor.laitosLiikuntabiologian laitosfi
dc.contributor.laitosDepartment of Biology of Physical Activityen
dc.contributor.oppiaineLiikuntafysiologiafi
dc.contributor.oppiaineExercise Physiologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2016-04-21T09:15:03Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.format.pagerange46-54
dc.relation.issn0531-5565
dc.relation.numberinseries0
dc.relation.volume79
dc.type.versionacceptedVersion
dc.rights.copyright© 2016 Elsevier Inc. This is a final draft version of an article whose final and definitive form has been published by Elsevier. Published in this repository with the kind permission of the publisher.
dc.rights.accesslevelopenAccessfi
dc.subject.ysooksidatiivinen stressi
jyx.subject.urihttp://www.yso.fi/onto/yso/p27309
dc.relation.doi10.1016/j.exger.2016.03.015


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