Show simple item record

dc.contributor.authorAsikainen, Suvi
dc.contributor.authorHeikkinen, Liisa
dc.contributor.authorJuhila, Juuso
dc.contributor.authorHolm, Frida
dc.contributor.authorWeltner, Jere
dc.contributor.authorTrokovic, Ras
dc.contributor.authorMikkola, Milla
dc.contributor.authorToivonen, Sanna
dc.contributor.authorBalboa, Diego
dc.contributor.authorLampela, Riina
dc.contributor.authorIcay, Katherine
dc.contributor.authorTuuri, Timo
dc.contributor.authorOtonkoski, Timo
dc.contributor.authorWong, Garry
dc.contributor.authorHovatta, Outi
dc.date.accessioned2015-05-21T08:48:13Z
dc.date.available2015-05-21T08:48:13Z
dc.date.issued2015
dc.identifier.citationAsikainen, S., Heikkinen, L., Juhila, J., Holm, F., Weltner, J., Trokovic, R., Mikkola, M., Toivonen, S., Balboa, D., Lampela, R., Icay, K., Tuuri, T., Otonkoski, T., Wong, G., & Hovatta, O. (2015). Selective MicroRNA-Offset RNA Expression in Human Embryonic Stem Cells. <i>PLoS ONE</i>, <i>10</i>(3), Article e0116668. <a href="https://doi.org/10.1371/journal.pone.0116668" target="_blank">https://doi.org/10.1371/journal.pone.0116668</a>
dc.identifier.otherCONVID_24712842
dc.identifier.otherTUTKAID_66174
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/45972
dc.description.abstractSmall RNA molecules, including microRNAs (miRNAs), play critical roles in regulating pluripotency, proliferation and differentiation of embryonic stem cells. miRNA-offset RNAs (moRNAs) are similar in length to miRNAs, align to miRNA precursor (pre-miRNA) loci and are therefore believed to derive from processing of the pre-miRNA hairpin sequence. Recent next generation sequencing (NGS) studies have reported the presence of moRNAs in human neurons and cancer cells and in several tissues in mouse, including pluripotent stem cells. In order to gain additional knowledge about human moRNAs and their putative development-related expression, we applied NGS of small RNAs in human embryonic stem cells (hESCs) and fibroblasts. We found that certain moRNA isoforms are notably expressed in hESCs from loci coding for stem cell-selective or cancer-related miRNA clusters. In contrast, we observed only sparse moRNAs in fibroblasts. Consistent with earlier findings, most of the observed moRNAs derived from conserved loci and their expression did not appear to correlate with the expression of the adjacent miRNAs. We provide here the first report of moRNAs in hESCs, and their expression profile in comparison to fibroblasts. Moreover, we expand the repertoire of hESC miRNAs. These findings provide an expansion on the known repertoire of small non-coding RNA contents in hESCs.
dc.language.isoeng
dc.publisherPublic Library of Science
dc.relation.ispartofseriesPLoS ONE
dc.subject.othermicroRNAs
dc.subject.othermiRNA-offset RNAs
dc.subject.otherRNA sequencing
dc.subject.otherembryonic stem cells
dc.titleSelective MicroRNA-Offset RNA Expression in Human Embryonic Stem Cells
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201505201923
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineEkologia ja evoluutiobiologiafi
dc.contributor.oppiaineEcology and Evolutionary Biologyen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2015-05-20T15:15:03Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.relation.issn1932-6203
dc.relation.numberinseries3
dc.relation.volume10
dc.type.versionpublishedVersion
dc.rights.copyright© 2015 Asikainen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.accesslevelopenAccessfi
dc.rights.urlhttp://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1371/journal.pone.0116668


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

© 2015 Asikainen et al. This is an open
access article distributed under the terms of the
Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any
medium, provided the original author and source are
credited.
Except where otherwise noted, this item's license is described as © 2015 Asikainen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.