Genetic relationship between clinical and environmental Vibrio cholerae isolates in Tanzania: A comparison using repetitive extragenic palindromic (REP) and enterobacterial repetitive intergenic consensus (ERIC) fingerprinting approach
Dalusi, L., Saarenheimo, J., Lyimo, T., & Lugomela, C. (2015). Genetic relationship between clinical and environmental Vibrio cholerae isolates in Tanzania: A comparison using repetitive extragenic palindromic (REP) and enterobacterial repetitive intergenic consensus (ERIC) fingerprinting approach. African journal of microbiology research, 9 (7), 455-462. doi:10.5897/AJMR2014.7307
Published inAfrican journal of microbiology research
© 2015 the Authors. Published by Academic Journals. This is an Open Access article under the terms of the Creative Commons Attribution Non Commercial License which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
The bacterium causing cholera, Vibrio cholerae, is a marine organism and coastal waters are important reservoirs of the organism. There are more than 200 serogroups of V. cholerae, of which serogroups O1 and O139 are known to be the causative agent of the cholera. The main virulent factor in V. cholerae is cholera toxin gene (ctx) that is found from the epidemic O1 and O139 strains, but may also be found in some strains other than O1 and O139 (non-O1 and non-O139). In this study, 48 V. cholerae strains isolated from three estuaries of Tanzania and 20 stool isolates were characterized in terms of their serogroups and possession of ctx gene and then compared using two PCR based fingerprinting methods: Enterobacterial repetitive intergenic consensus (ERIC) sequences and repetitive extragenic palindromic (REP) sequences. All the stool isolates and twelve of the environmental isolates belonged to serogroup O1 while the remaining 36 environmental isolates were defined as non-O1/O139. The entire stool isolates and 21 of the environmental isolates had the cholera toxin gene (ctxA). Both ERIC and REP methods gave almost unique fingerprints for each strain and confirmed high genetic heterogeneity among the different cholera strains. Higher similarity was observed in REP-PCR (70-100%) than in ERIC-PCR (62-100%), indicating different discriminative power of these methods. Environmental isolates clustered together with clinical isolates at ≥90% similarity level suggesting their great potential of producing pathogenic strains that may be the causative agents for the frequent observed cholera outbreaks particularly along the coast. ...
MetadataShow full item record
Except where otherwise noted, this item's license is described as © 2015 the Authors. Published by Academic Journals. This is an Open Access article under the terms of the Creative Commons Attribution Non Commercial License which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Showing items with similar title or keywords.
Relationship between physical activity and predicted home presenteeism among participants with depressive symptoms with and without clinical depression : Findings from Finnish Depression and Metabolic Syndrome in Adults (FDMSA) study Raatikainen, I.; Vanhala, M.; Mäntyselkä, P.; Heinonen, A.; Kautiainen, H.; Koponen, H.; Korniloff, K. (Elsevier; Asociación Universitaria de Zaragoza para el Progreso de la Psiquiatría y la Salud Mental, 2021)Background and objectives Depression can pose a major threat to an individual’s ability to cope with daily activities. The aim of this study was to explore the relationship between physical activity (PA) and predicted ...
European evidence-based recommendations for clinical assessment of upper limb in neurorehabilitation (CAULIN) : data synthesis from systematic reviews, clinical practice guidelines and expert consensus Prange-Lasonder, Gerdienke B.; Alt Murphy, Margit; Lamers, Ilse; Hughes, Ann-Marie; Buurke, Jaap H.; Feys, Peter; Keller, Thierry; Klamroth-Marganska, Verena; Tarkka, Ina M.; Timmermans, Annick; Burridge, Jane H. (Biomed Central, 2021)Background: Technology-supported rehabilitation can help alleviate the increasing need for cost-effective rehabilitation of neurological conditions, but use in clinical practice remains limited. Agreement on a core set of ...
The Relationship Between Ethical Organisational Culture and Organisational Innovativeness : Comparison of Findings from Finland and Lithuania Pučėtaitė, Raminta; Novelskaitė, Aurelija; Lämsä, Anna-Maija; Riivari, Elina (Springer Netherlands, 2016)The paper explores the interrelations between ethical organisational culture and organisational innovativeness in two different socio-cultural contexts, Finland and Lithuania. According to the Global Innovation Index 2013, ...
Energy-Efficient Edge Computing Service Provisioning for Vehicular Networks : A Consensus ADMM Approach Zhou, Zhenyu; Feng, Junhao; Chang, Zheng; Shen, Xuemin Sherman (Institute of Electrical and Electronics Engineers, 2019)In vehicular networks, in-vehicle user equipment (UE) with limited battery capacity can achieve opportunistic energy saving by offloading energy-hungry workloads to vehicular edge computing nodes via vehicle-to-infrastructure ...