Sequencing, De Novo Assembly and Annotation of the Colorado Potato Beetle, Leptinotarsa decemlineata, Transcriptome
Kumar, A., Congiu, L., Lindström, L., Piiroinen, S., Vidotto, M., & Grapputo, A. (2014). Sequencing, De Novo Assembly and Annotation of the Colorado Potato Beetle, Leptinotarsa decemlineata, Transcriptome. PLOS ONE, 9(1). https://doi.org/10.1371/journal.pone.0086012
Published inPLOS ONE
DisciplineEkologia ja evoluutiobiologiaBiologisten vuorovaikutusten huippututkimusyksikköEcology and Evolutionary BiologyCentre of Excellence in Biological Interactions Research
© 2014 Kumar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background. The Colorado potato beetle (Leptinotarsa decemlineata) is a major pest and a serious threat to potato cultivation throughout the northern hemisphere. Despite its high importance for invasion biology, phenology and pest management, little is known about L. decemlineata from a genomic perspective. We subjected European L. decemlineata adult and larval transcriptome samples to 454-FLX massively-parallel DNA sequencing to characterize a basal set of genes from this species. We created a combined assembly of the adult and larval datasets including the publicly available midgut larval Roche 454 reads and provided basic annotation. We were particularly interested in diapause-specific genes and genes involved in pesticide and Bacillus thuringiensis (Bt) resistance. Results. Using 454-FLX pyrosequencing, we obtained a total of 898,048 reads which, together with the publicly available 804,056 midgut larval reads, were assembled into 121,912 contigs. We established a repository of genes of interest, with 101 out of the 108 diapause-specific genes described in Drosophila montana; and 621 contigs involved in insecticide resistance, including 221 CYP450, 45 GSTs, 13 catalases, 15 superoxide dismutases, 22 glutathione peroxidases, 194 esterases, 3 ADAM metalloproteases, 10 cadherins and 98 calmodulins. We found 460 putative miRNAs and we predicted a significant number of single nucleotide polymorphisms (29,205) and microsatellite loci (17,284). Conclusions. This report of the assembly and annotation of the transcriptome of L. decemlineata offers new insights into diapause-associated and insecticide-resistance-associated genes in this species and provides a foundation for comparative studies with other species of insects. The data will also open new avenues for researchers using L. decemlineata as a model species, and for pest management research. Our results provide the basis for performing future gene expression and functional analysis in L. decemlineata and improve our understanding of the biology of this invasive species at the molecular level. ...
PublisherPublic Library of Science
Publication in research information system
MetadataShow full item record
Showing items with similar title or keywords.
Ecological and genetic factors contributing to invasion success : the northern spread of the Colorado potato beetle (Leptinotarsa decemlineata) Boman, Sanna (University of Jyväskylä, 2008)Sanna Boman tutki väitöskirjassaan koloradonkuoriaisen potentiaalia levitä Suomeen. Hän selvitti kuoriaisten mahdollisuuksia selviytyä alhaisissa lämpötiloissa ja kykyä sopeutua Suomen viileään ilmastoon.Boman havaitsi, ...
Adaptation to stressful environments : invasion success of the Colorado potato beetle (Leptinotarsa decemlineata) Margus, Aigi (Jyväskylän yliopisto, 2018)Biological invasions, specifically human-induced dispersals, are one of the major threats to our biodiversity and are predicted to increase. Invasive pests provide an opportunity to study whether adaptation to human-induced ...
Effects of a glyphosate-based herbicide on survival and oxidative status of a non-target herbivore, the Colorado potato beetle (Leptinotarsa decemlineata) Rainio, Miia J.; Margus, Aigi; Lehmann, Philipp; Helander, Marjo; Lindström, Leena (Elsevier Inc., 2019)Glyphosate is the globally most used herbicide against a wide range of weeds. Glyphosate has been considered safe to animals as it mainly targets physiological pathways in plants. However, recent toxicological studies have ...
Northward range expansion requires synchronization of both overwintering behaviour and physiology with photoperiod in the invasive Colorado potato beetle (Leptinotarsa decemlineata) Lehmann, Philipp; Lyytinen, Anne; Piiroinen, Saija; Lindström, Leena (Springer; International Association for Ecology, (Intecol), 2014)Abstract. Photoperiodic phenological adaptations are prevalent in many organisms living in seasonal environments. As both photoperiod and growth season length change with latitude, species undergoing latitudinal range ...
Range expansion to novel environments : evolutionary physiology and genetics in Leptinotarsa decemlineata Piiroinen, Saija (University of Jyväskylä, 2010)