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ICTV Virus Taxonomy Profile: Simuloviridae 2023
(Microbiology Society, 2023)
The family Simuloviridae includes tailless icosahedral viruses with an internal lipid membrane. The capsid is constructed from two major capsid proteins, both with a single jelly-roll fold. The genome is a circular dsDNA ...
ICTV Virus Taxonomy Profile: Sphaerolipoviridae 2023
(Microbiology Society, 2023)
Members of the family Sphaerolipoviridae have non-enveloped tailless icosahedral virions with a protein-rich internal lipid membrane. The genome is a linear double-stranded DNA of about 30 kbp with inverted terminal repeats ...
Lack of evidence of mimivirus replication in human PBMCs
(Elsevier Masson, 2018)
The Acanthamoeba polyphaga mimivirus (APMV) was first isolated during a pneumonia outbreak in Bradford, England, and since its discovery many research groups devoted efforts to understand whether this virus could be ...
Lukiolaisten tiedot, virhekäsitykset ja tunteet : aiheena tautia-aiheuttavat virukset ja rokotteet
(2021)
Virukset ovat vakava globaali terveysuhka. Kilpajuoksu viruksia vastaan edellyttää jokaiselta ihmiseltä tietoon pohjautuvia tekoja ja valintoja. Kun tiedetään, millainen tietopohja taudinaiheuttajista nuorilla on Suomessa, osataan biologian opetusta...
The viruses pose a serious global health threat. The arms race against viruses demands evidence-based actions and choices from everyone. When it is known what kind of knowledge today’s youth have on such topics, biology teaching can be more focused in the future. The aim of this study was to find out whether high school first year students’ knowledge about viruses, vaccines and the COVID-19 pandemic was based on scientific facts and what kinds of potential misconceptions they had. The aim was also to study what kinds of feelings the pandemic had caused among the students. Six high school groups answered an internet-based questionnaire and their biology teachers were interviewed. The results showed that the students’ knowledge on the biology of viruses and vaccines was variable. The students had fairly good knowledge on the structure and characteristics of viruses and vaccines but clearly weaker knowledge on interaction between viruses and cells and viral replication. Common misconceptions were related to e.g. viral penetration and replication and the function of vaccines. The students’ understanding on the COVID-19 pandemic mostly coincided with scientific facts. The pandemic had caused feelings of frustration, irritation, worry and anxiety, but also hope. In high schools, more attention should be paid on common misconceptions about viruses and vaccines because those can restrict understanding and absorption of new scientific knowledge about viruses and vaccines in the future....
The viruses pose a serious global health threat. The arms race against viruses demands evidence-based actions and choices from everyone. When it is known what kind of knowledge today’s youth have on such topics, biology teaching can be more focused in the future. The aim of this study was to find out whether high school first year students’ knowledge about viruses, vaccines and the COVID-19 pandemic was based on scientific facts and what kinds of potential misconceptions they had. The aim was also to study what kinds of feelings the pandemic had caused among the students. Six high school groups answered an internet-based questionnaire and their biology teachers were interviewed. The results showed that the students’ knowledge on the biology of viruses and vaccines was variable. The students had fairly good knowledge on the structure and characteristics of viruses and vaccines but clearly weaker knowledge on interaction between viruses and cells and viral replication. Common misconceptions were related to e.g. viral penetration and replication and the function of vaccines. The students’ understanding on the COVID-19 pandemic mostly coincided with scientific facts. The pandemic had caused feelings of frustration, irritation, worry and anxiety, but also hope. In high schools, more attention should be paid on common misconceptions about viruses and vaccines because those can restrict understanding and absorption of new scientific knowledge about viruses and vaccines in the future....
Silent Rain : Does the atmosphere-mediated connectivity between microbiomes influence bacterial evolutionary rates?
(Oxford University Press, 2020)
Air carries a vast number of bacteria and viruses over great distances all the time. This leads into the continuous introduction of foreign genetic material to local established microbial communities. In this perspective, ...
Foldameerien lääketieteelliset käyttösovellukset
(2019)
Kirjallisuuskatsauksessa tutkitaan foldameerien lääketieteellisiä käyttösovelluksia. Tässä työssä myös
perehdytään foldameerien perusrakenteisiin ja foldameerien luokitteluun abioottisiksi tai bioottisiksi.
Lääketieteen ...
The effect of PLC-inhibitor on echovirus 1 internalization probed with fluorescently labeled echovirus 1
(2014)
Echovirus 1 (EV1) kuuluu Pikornavirusperheeseen ja tarkemmin luokiteltuna Enterovirusten sukuun. EV1 on pieni ja vaipaton RNA-virus, jonka proteiinikuori koostuu neljästä proteiinista VP1-VP4. Infektion on osoitettu alkavan, ...
Proteiini IX:n yliekspression vaikutus adenovirusvektoreiden stabiilisuuteen ja infektiivisyyteen
(2019)
Geeniterapiassa sairautta hoidetaan siirtämällä soluihin siirtogeeni, joka koodaa haluttua proteiinituotetta. Virukset toimivat hyvinä geenien kuljettimina eli vektoreina, sillä niille on luontaista siirtää perimänsä kohdesolun replikoitavaksi...
In gene therapy genes are used to treat or cure illnesses. These transgenes are delivered into patient's cells with a special tool, vector. Viruses are often used as vectors because of their natural ability to transfer their genes into target cells. Adenovirus is a very common vector in gene therapy because its features are well known and it can infect many different types of cells in various times in cell cycle. Adenovirus structural protein IX (pIX) is located between hexon proteins stabilizing the structure of adenoviral capsid. Studies indicate that pIX might also have additional roles during adenovirus infection. In this study the object was to find out how the stabilities and infectivities of two different adenoviral vectors differ from each other. The aim was also to find out if the amount of pIX can be increased in adenoviral capsid by overexpressing it in the producer cells and if this has an effect to the stability of the capsid. Both vectors were produced with and without pIX overexpression. The effects of pIX overexpression to stability were studied by treating the viruses in different ways and observing the differences in infectivities. Produced vectors were purified using ultracentrifugation and dialysis. The purity and amount of pIX were estimated using Coomassie staining and Western blot analysis. Virus titers were determined using ddPCR (digital droplet polymerase chain reaction). The infectivities were determined by infecting producer cells for 2 hours and staining the cells after 48 hours with anti-pIX antibody. Results were analyzed using flow cytometry. Although statistically significant differences couldn't be observed in stability studies, the viruses which were produced with overexpression of pIX were more infectious than the ones that had been produced in normal producer cells. These results can be utilized when designing more effective and durable viral vectors for the field of gene therapy....
In gene therapy genes are used to treat or cure illnesses. These transgenes are delivered into patient's cells with a special tool, vector. Viruses are often used as vectors because of their natural ability to transfer their genes into target cells. Adenovirus is a very common vector in gene therapy because its features are well known and it can infect many different types of cells in various times in cell cycle. Adenovirus structural protein IX (pIX) is located between hexon proteins stabilizing the structure of adenoviral capsid. Studies indicate that pIX might also have additional roles during adenovirus infection. In this study the object was to find out how the stabilities and infectivities of two different adenoviral vectors differ from each other. The aim was also to find out if the amount of pIX can be increased in adenoviral capsid by overexpressing it in the producer cells and if this has an effect to the stability of the capsid. Both vectors were produced with and without pIX overexpression. The effects of pIX overexpression to stability were studied by treating the viruses in different ways and observing the differences in infectivities. Produced vectors were purified using ultracentrifugation and dialysis. The purity and amount of pIX were estimated using Coomassie staining and Western blot analysis. Virus titers were determined using ddPCR (digital droplet polymerase chain reaction). The infectivities were determined by infecting producer cells for 2 hours and staining the cells after 48 hours with anti-pIX antibody. Results were analyzed using flow cytometry. Although statistically significant differences couldn't be observed in stability studies, the viruses which were produced with overexpression of pIX were more infectious than the ones that had been produced in normal producer cells. These results can be utilized when designing more effective and durable viral vectors for the field of gene therapy....