Estimating Cecidomyiidae (Diptera) species richness in Finland using DNA barcoding
Authors
Date
2022Access restrictions
The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyväskylä University Library (https://kirjasto.jyu.fi/collections/archival-workstation).
Cecidomyiidae is a hyperdiverse but inadequately studied dipteran family. The number of described Cecidomyiidae species in the world is 6600 but the true species count is still regarded as unknown. Based on DNA barcodes, the number of Cecidomyiidae species in the world is estimated to be as high as 1.8 million. In Finland, no thorough inventories of Cecidomyiidae have been conducted, and the known species count is 384. The purpose of this study was to estimate the expected number of Cecidomyiidae species occurring in Finland utilising BINs (Barcode Index Number) for preliminary species delimitation. Cecidomyiids were collected from eight sites across the country using Malaise traps in the summer of 2020. Supplementary material from 13 sites was also included to the study. The barcode fragment of the COI (cytochrome oxidase c subunit I) gene was sequenced from 9025 Cecidomyiidae specimens. Of the analysed specimens, 7838 recovered validated sequences and were assigned to 1420 BINs. Species count was estimated using model-based extrapolations from rarefaction curves of the abundance data. Whole material is estimated to contain 1.8-fold species count compared to the analysed proportion (⅓). Since further extrapolated rarefaction curve did not reach an asymptote, the estimate of the number of Cecidomyiidae species in Finland still remains largely unknown. However, Finnish Cecidomyiidae fauna evidently consist of thousands of species. The result is congruent with previous studies and points out the efficiency of DNA based methods in dark taxa research.
...
Keywords
Metadata
Show full item recordCollections
- Pro gradu -tutkielmat [29684]
License
Related items
Showing items with similar title or keywords.
-
Resolving biology’s dark matter : species richness, spatiotemporal distribution, and community composition of a dark taxon
Hartop, Emily; Lee, Leshon; Srivathsan, Amrita; Jones, Mirkka; Peña-Aguilera, Pablo; Ovaskainen, Otso; Roslin, Tomas; Meier, Rudolf (BioMed Central, 2024)Background Zoology’s dark matter comprises hyperdiverse, poorly known taxa that are numerically dominant but largely unstudied, even in temperate regions where charismatic taxa are well understood. Dark taxa are everywhere, ... -
Host specificity of Selenidium pygospionis (Archigregarine Apicomplexa)
Alale, Theophilus (2019)In order to understand potential co-evolution between a parasite and its host, this study was conducted to investigate whether the apicomplexan parasite Selenidium pygospionis, specifically infects the polychaete worm, ... -
DNA barcoding reveals different cestode helminth species in northern European marine and freshwater ringed seals
Nyman, Tommi; Papadopoulou, Elena; Ylinen, Eeva; Wutke, Saskia; Michell, Craig T.; Sromek, Ludmila; Sinisalo, Tuula; Andrievskaya, Elena; Alexeev, Vyacheslav; Kunnasranta, Mervi (Elsevier Ltd., 2021)Three subspecies of the ringed seal (Pusa hispida) are found in northeastern Europe: P. h. botnica in the Baltic Sea, P. h saimensis in Lake Saimaa in Finland, and P. h. ladogensis in Lake Ladoga in Russia. We investigated ... -
Integrative taxonomy resolves species identities within the Macrobiotus pallarii complex (Eutardigrada: Macrobiotidae)
Stec, Daniel; Vecchi, Matteo; Dudziak, Magdalena; Bartels, Paul J.; Calhim, Sara; Michalczyk, Łukasz (Biomed Central, 2021)The taxonomy of many groups of meiofauna is challenging due to their low number of diagnostic morphological characters and their small body size. Therefore, with the advent of molecular techniques that provide a new source ... -
Desiccation risk favours prevalence and diversity of tardigrade communities and influences their trophic structure in alpine ephemeral rock pools
Vecchi, Matteo; Ferrari, Claudio; Stec, Daniel; Calhim, Sara (Springer, 2022)Rock pools are ephemeral freshwater habitats characterized by their small size, well-defined boundaries, and periodic desiccation, making them ideal model systems to answer numerous ecological questions. Although there are ...