Effect of acute strength training on cellular respiration and gene expression of peripheral blood mononuclear cells
Authors
Date
2019Access restrictions
The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyväskylä University Library (https://kirjasto.jyu.fi/en/workspaces/facilities).
Copyright
This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.
Liikunnan avulla voidaan ehkäistä matala-asteista tulehdustilaa, joka on osallisena monien sairauksien kehittymisessä. Toisaalta liikunta voi aiheuttaa lihassoluihin mikrovaurioita laukaisten kehossa tulehdusvasteen, jonka välittäjänä toimivat mononukleaariset valkosolut. Näiden valkosolujen mitokondrioiden toimintaa pidetään yhtenä mahdollisena terveysindikaattorina ja tämä voi myös olla hyödyllinen tutkittaessa liikunnan vaikutuksia terveyteen. Voimaharjoittelun sekä eksentrisen ja konsentrisen lihaskuormitusten eroja valkosolujen soluhengitykseen ei ole tutkittu. Tässä tutkimuksessa kaksitoista miesurheilijaa suoritti eksentrisen, konsentrisen ja näiden yhdistelmäharjoitteen satunnaisessa järjestyksessä. Verinäytteet otettiin ennen harjoitusta sekä 5 min ja 24 tuntia harjoituksen jälkeen. Mononukleaariset valkosolut eristettiin soluhengitysmittauksia varten, jotka suoritettiin korkean erotuskyvyn respirometrillä. Hengitystasot määritettiin hapen kulutuksen perusteella. Rutiinihengitys, elektroninsiirtokapasiteetti sekä vapaa rutiinihengityksen kapasiteetti muuttuivat 5-min yhdistelmäharjoitteen jälkeen. Eksentrisen kuormituksen vaikutus soluhengitykseen oli erilainen verrattuna toisiin kuormituksiin. Tulehdukseen ja mitokondrioihin liittyvien geenin ekpressiotasoissa ei löydetty huomattavaa muutosta. Akuutilla eksentrisellä voimaharjoituksella on erilainen vaikutus valkosoluihin, kuin akuutilla konsentrisella ja yhdistelmäharjoituksella, mikä voi johtua suuremmasta lihasvauriomäärästä, tulehdusvasteesta ja lihaksiston kuormittumisesta.
...
Physical activity prevents chronic low-grade inflammatory state which predisposes to many diseases. Exercise causes micro damage to muscle fibers triggering a temporary inflammatory response that is mediated by peripheral blood mononuclear cells (PBMCs) which are important part of the immune system. Mitochondrial bioenergetics of PBMCs could be a useful health indicator when investigating effects of exercise. Acute effects of resistance training on cellular respiration of PBMCs has not been studied, neither if eccentric and concentric contractions have different impact on it. For investigating this, twelve young male athletes performed eccentric (ECC), concentric (CON) and combination (CEX) of these two exercises in a random order. Blood samples were taken prior exercise, 5 min and 24 hours after and PBMCs were isolated for the respiration measurements with high resolution respirometry. Respiration states were analyzed from the real-time oxygen concentration data. Routine respiration, electron transfer capacity (ET) and free routine capacity showed significant changes after CEX and between different resistance exercises. These changes were reflected in routine control ratio and excess E-R capacity factor. Expression of four genes of PBMCs related to inflammation and mitochondrial dynamics did not show statistical significant changes on them. Acute ECC has different impact on cellular respiration of PBMCs than CON and CEX which may be a result of higher muscle damage level, higher inflammation response and fatigue of muscles.
...
Keywords
Metadata
Show full item recordCollections
- Pro gradu -tutkielmat [29473]
Related items
Showing items with similar title or keywords.
-
Resistance exercise with different workloads have distinct effects on cellular respiration of peripheral blood mononuclear cells
Lähteenmäki, Emilia Ilona; Koski, Max; Koskela, Iida; Lehtonen, Elias; Kankaanpää, Anna; Kainulainen, Heikki; Walker, Simon; Lehti, Maarit (Wiley, 2022)Little is known how acute exercise-induced inflammation and metabolic stress affect immune cell bioenergetics and the portion of its components. Therefore, we investigated acute effects of eccentric-only (E), concentric-only ... -
Effects of eccentric and concentric strength training on maximum strength, power variables and dynamic strength index
Rajalainen, Ville (2021)Eccentric and concentric strength training have been studied and compared since 1890’s. Studies have reported mixing results regarding strength gains. Also, research focusing on the effects of these training modalities on ... -
Effects of Upper Body Eccentric versus Concentric Strength Training and Detraining on Maximal Force, Muscle Activation, Hypertrophy and Serum Hormones in Women
Häkkinen, Keijo; Newton, Robert U.; Walker, Simon; Häkkinen, Arja; Krapi, Sonja; Rekola, Rebekka; Koponen, Päivi; Kraemer, William J.; Haff, G. Gregory; Blazevich, Anthony J.; Nosaka, Kazunori; Ahtiainen, Juha (Uludag University, 2022)Effects of eccentric (ECC) versus concentric (CON) strength training of the upper body performed twice a week for 10 weeks followed by detraining for five weeks on maximal force, muscle activation, muscle mass and serum ... -
Effects of eccentric and concentric isokinetic bench press training on dynamic strength, isometric force production and triceps brachii cross-sectional area
Wanttaja, Ryan (2020)Background. Engaging in resistance training programmes is an integral part of the physical preparation process for athletes. The neural and morphological mechanisms underpinning eccentric contractions are notably different ...