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dc.contributor.advisorKnott, Emily
dc.contributor.authorAlale, Theophilus
dc.date.accessioned2019-07-04T04:54:38Z
dc.date.available2019-07-04T04:54:38Z
dc.date.issued2019
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/64978
dc.description.abstractIn order to understand potential co-evolution between a parasite and its host, this study was conducted to investigate whether the apicomplexan parasite Selenidium pygospionis, specifically infects the polychaete worm, Pygospio elegans or if it has a broader distribution in other polychaete species. In addition, I analyzed the diversity of the mitochondrial cytochrome c oxidase subunit I (COX-I) gene in both the host and parasite from different populations in Europe. Species specific primers were used in ddPCR to identify the presence of S. pygospionis in polychaete species obtained from Finland, Scotland, Denmark and Iceland. With additional species specific primer pairs, ABI Sanger sequencing method was used to generate 548 bp sequences from the apicomplexan COX-I gene. Genetic diversity in COX-I was compared to that determined from DNA sequence data from P. elegans in order to compare the genetic diversity between the host (P. elegans) and the parasite (S. pygospionis). The ddPCR assay suggested that Marenzelleria species had higher levels of amplified apicomplexan COX-I compared to other species sampled. However, sequences obtained from the amplicon did not verify the result. The genetic diversity for both species was low as was the interpopulation and intrapopulation genetic distances. These results indicate that S. pygospionis is a specific parasite of Pygospio elegans despite the fact that other species are likely exposed to the parasite’s oocysts in the environment. Moreover, the COX-I gene may not be sufficient to separate different Selenidium species. It will therefore require that other coding genes such as Cytochrome b (Cytb) and COX-III be tried before conclusive inference.en
dc.format.extent47
dc.language.isoen
dc.subject.otherCo-evolution
dc.subject.otherCOX-I
dc.subject.otherMarine gregarines
dc.subject.otherDNA barcoding
dc.subject.otherddPCR
dc.titleHost specificity of Selenidium pygospionis (Archigregarine Apicomplexa)
dc.identifier.urnURN:NBN:fi:jyu-201907043568
dc.type.ontasotMaster’s thesisen
dc.type.ontasotPro gradu -tutkielmafi
dc.contributor.tiedekuntaMatemaattis-luonnontieteellinen tiedekuntafi
dc.contributor.tiedekuntaFaculty of Sciencesen
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.yliopistoJyväskylän yliopistofi
dc.contributor.yliopistoUniversity of Jyväskyläen
dc.contributor.oppiaineEkologia ja evoluutiobiologiafi
dc.contributor.oppiaineEcology and evolutionary biologyen
dc.rights.copyrightJulkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.fi
dc.rights.copyrightThis publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.en
dc.contributor.oppiainekoodi4011
dc.subject.ysoloiset
dc.subject.ysoparasitismi
dc.subject.ysoDNA-viivakoodit
dc.subject.ysoisäntälajit
dc.subject.ysoDNA-analyysi
dc.subject.ysoparasites
dc.subject.ysoparasitism
dc.subject.ysoDNA barcodes
dc.subject.ysohost species
dc.subject.ysoDNA analysis
dc.rights.accessrightsTekijä ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyväskylän yliopiston kirjaston arkistotyösemalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat..fi
dc.rights.accessrightsThe author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyväskylä University Library (https://kirjasto.jyu.fi/en/workspaces/facilities).en


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