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dc.contributor.authorReshamwala, Dhanik
dc.contributor.authorShroff, Sailee
dc.contributor.authorSheik, Amamuddy Olivier
dc.contributor.authorLaquintana, Valentino
dc.contributor.authorDenora, Nunzio
dc.contributor.authorZacheo, Antonella
dc.contributor.authorLampinen, Vili
dc.contributor.authorHytonen, Vesa P.
dc.contributor.authorTastan, Bishop Özlem
dc.contributor.authorKrol, Silke
dc.contributor.authorMarjomäki, Varpu
dc.date.accessioned2021-08-04T12:04:00Z
dc.date.available2021-08-04T12:04:00Z
dc.date.issued2021
dc.identifier.citationReshamwala, D., Shroff, S., Sheik, A. O., Laquintana, V., Denora, N., Zacheo, A., Lampinen, V., Hytonen, V. P., Tastan, B. Ö., Krol, S., & Marjomäki, V. (2021). Polyphenols Epigallocatechin Gallate and Resveratrol, and Polyphenol-Functionalized Nanoparticles Prevent Enterovirus Infection through Clustering and Stabilization of the Viruses. <i>Pharmaceutics</i>, <i>13</i>(8), Article 1182. <a href="https://doi.org/10.3390/pharmaceutics13081182" target="_blank">https://doi.org/10.3390/pharmaceutics13081182</a>
dc.identifier.otherCONVID_99193030
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/77281
dc.description.abstractTo efficiently lower virus infectivity and combat virus epidemics or pandemics, it is important to discover broadly acting antivirals. Here, we investigated two naturally occurring polyphenols, Epigallocatechin gallate (EGCG) and Resveratrol (RES), and polyphenol-functionalized nanoparticles for their antiviral efficacy. Concentrations in the low micromolar range permanently inhibited the infectivity of high doses of enteroviruses (107 PFU/mL). Sucrose gradient separation of radiolabeled viruses, dynamic light scattering, transmission electron microscopic imaging and an in-house developed real-time fluorescence assay revealed that polyphenols prevented infection mainly through clustering of the virions into very stable assemblies. Clustering and stabilization were not compromised even in dilute virus solutions or after diluting the polyphenols-clustered virions by 50-fold. In addition, the polyphenols lowered virus binding on cells. In silico docking experiments of these molecules against 2- and 3-fold symmetry axes of the capsid, using an algorithm developed for this study, discovered five binding sites for polyphenols, out of which three were novel binding sites. Our results altogether suggest that polyphenols exert their antiviral effect through binding to multiple sites on the virion surface, leading to aggregation of the virions and preventing RNA release and reducing cell surface binding.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofseriesPharmaceutics
dc.rightsCC BY 4.0
dc.subject.otherpolyphenols
dc.subject.otherfunctionalized gold nanoparticles
dc.subject.otherantiviral efficacy
dc.subject.otherenteroviruses
dc.subject.otherstabilization
dc.titlePolyphenols Epigallocatechin Gallate and Resveratrol, and Polyphenol-Functionalized Nanoparticles Prevent Enterovirus Infection through Clustering and Stabilization of the Viruses
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202108044449
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn1999-4923
dc.relation.numberinseries8
dc.relation.volume13
dc.type.versionpublishedVersion
dc.rights.copyright© 2021 the Authors
dc.rights.accesslevelopenAccessfi
dc.subject.ysonanohiukkaset
dc.subject.ysoinfektiot
dc.subject.ysolääkehoito
dc.subject.ysovirukset
dc.subject.ysopandemiat
dc.subject.ysoepidemiat
dc.subject.ysostabilointi (kemia)
dc.subject.ysopolyfenolit
dc.subject.ysotorjunta
dc.subject.ysoenterovirukset
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p23451
jyx.subject.urihttp://www.yso.fi/onto/yso/p7316
jyx.subject.urihttp://www.yso.fi/onto/yso/p10851
jyx.subject.urihttp://www.yso.fi/onto/yso/p1123
jyx.subject.urihttp://www.yso.fi/onto/yso/p10121
jyx.subject.urihttp://www.yso.fi/onto/yso/p12979
jyx.subject.urihttp://www.yso.fi/onto/yso/p977
jyx.subject.urihttp://www.yso.fi/onto/yso/p8756
jyx.subject.urihttp://www.yso.fi/onto/yso/p792
jyx.subject.urihttp://www.yso.fi/onto/yso/p20689
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3390/pharmaceutics13081182
jyx.fundinginformationThis work was supported by the Jane and Aatos Erkko foundation (V.M.).
dc.type.okmA1


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