Comparative analysis of two paradigm bacteriophytochromes reveals opposite functionalities in two-component signaling
Multamäki, E., Nanekar, R., Morozov, D., Lievonen, T., Golonka, D., Wahlgren, W. Y., Stucki-Buchli, B., Rossi, J., Hytönen, V. P., Westenhoff, S., Ihalainen, J. A., Möglich, A., & Takala, H. (2021). Comparative analysis of two paradigm bacteriophytochromes reveals opposite functionalities in two-component signaling. Nature Communications, 12, Article 4394. https://doi.org/10.1038/s41467-021-24676-7
Published in
Nature CommunicationsAuthors
Date
2021Discipline
Nanoscience CenterFysikaalinen kemiaSolu- ja molekyylibiologiaNanoscience CenterPhysical ChemistryCell and Molecular BiologyCopyright
© The Author(s) 2021
Bacterial phytochrome photoreceptors usually belong to two-component signaling systems which transmit environmental stimuli to a response regulator through a histidine kinase domain. Phytochromes switch between red light-absorbing and far-red light-absorbing states. Despite exhibiting extensive structural responses during this transition, the model bacteriophytochrome from Deinococcus radiodurans (DrBphP) lacks detectable kinase activity. Here, we resolve this long-standing conundrum by comparatively analyzing the interactions and output activities of DrBphP and a bacteriophytochrome from Agrobacterium fabrum (Agp1). Whereas Agp1 acts as a conventional histidine kinase, we identify DrBphP as a light-sensitive phosphatase. While Agp1 binds its cognate response regulator only transiently, DrBphP does so strongly, which is rationalized at the structural level. Our data pinpoint two key residues affecting the balance between kinase and phosphatase activities, which immediately bears on photoreception and two-component signaling. The opposing output activities in two highly similar bacteriophytochromes suggest the use of light-controllable histidine kinases and phosphatases for optogenetics.
...
Publisher
Springer Science and Business Media LLCISSN Search the Publication Forum
2041-1723Keywords
Publication in research information system
https://converis.jyu.fi/converis/portal/detail/Publication/99079024
Metadata
Show full item recordCollections
Related funder(s)
Research Council of Finland; European Commission; Jane and Aatos Erkko FoundationFunding program(s)
Academy Research Fellow, AoF; Research infrastructures, H2020; Academy Project, AoF; Foundation
The content of the publication reflects only the author’s view. The funder is not responsible for any use that may be made of the information it contains.
Additional information about funding
This work was supported by Academy of Finland grants 285461, 330678 (H.T.), 296135, and 332742 (J.A.I.), Jane and Aatos Erkko Foundation (J.A.I.), Three-year grant 2018–2020 from the University of Helsinki (E.M. and H.T.), and Bayreuth Humboldt Centre Senior Fellowship 2020 (E.M. and H.T.). S.W. and W.W. acknowledge the European Research Council for support (grant number: 279944), and B.S.-B. acknowledges Swiss National Science Foundation (P2ZHP2_164991). D.M. acknowledge the BioExcel CoE (www.bioexcel.eu), funded by the European Union contracts H2020-INFRAEDI-02-2018-823830, H2020-EINFRA-2015-1-675728. ...License
Related items
Showing items with similar title or keywords.
-
Optogenetic Control of Bacterial Expression by Red Light
Multamäki, Elina; García de Fuentes, Andrés; Sieryi, Oleksii; Bykov, Alexander; Gerken, Uwe; Ranzani, Américo Tavares; Köhler, Jürgen; Meglinski, Igor; Möglich, Andreas; Takala, Heikki (American Chemical Society (ACS), 2022)In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle ... -
Multimodal Control of Bacterial Gene Expression by Red and Blue Light
Meier, Stefanie S. M.; Multamäki, Elina; Ranzani, Américo T.; Takala, Heikki; Möglich, Andreas (Humana Press, 2024)By applying sensory photoreceptors, optogenetics realizes the light-dependent control of cellular events and state. Given reversibility, noninvasiveness, and exquisite spatiotemporal precision, optogenetic approaches enable ... -
The effects of photosensory module components for phytochrome activity and spectroscopy
Vanhatalo, Roosa (2023)Bakteerifytokromit voivat säädellä isäntäsolun sisäistä metaboliaa valo-olosuhteista riippuen. Tämän mahdollistaa se, että fytokromit voivat toimia osana kaksikomponenttijärjestelmiä, jotka voivat puolestaan säädellä geenien ... -
On the (un)coupling of the chromophore, tongue interactions and overall conformation in a bacterial phytochrome
Takala, Heikki; Lehtivuori, Heli; Berntsson, Oskar; Hughes, Ashley; Nanekar, Rahul; Niebling, Stephan; Panman, Matthijs; Henry, Léocadie; Menzel, Andreas; Westenhoff, Sebastian; Ihalainen, Janne (American Society for Biochemistry and Molecular Biology, Inc., 2018)Phytochromes are photoreceptors in plants, fungi, and various microorganisms and cycle between metastable red light–absorbing (Pr) and far-red light–absorbing (Pfr) states. Their light responses are thought to follow a ... -
Structural basis for light control of cell development revealed by crystal structures of a myxobacterial phytochrome
Woitowich, Nicole C.; Halavaty, Andrei S.; Waltz, Patricia; Kupitz, Christopher; Valera, Joseph; Tracy, Gregory; Gallagher, Kevin D.; Claesson, Elin; Nakane, Takanori; Pandey, Suraj; Nelson, Garrett; Tanaka, Rie; Nango, Eriko; Mizohata, Eiichi; Owada, Shigeki; Tono, Kensure; Joti, Yasumasa; Nugent, Angela C.; Patel, Hardik; Mapara, Ayesha; Hopkins, James; Duong, Phu; Bizhga, Dorina; Kovaleva, Svetlana E.; Peter, Rachael St.; Hernandez, Cynthia N.; Ozarowski, Wesley B.; Roy-Chowdhuri, Shatabdi; Yang, Jay-How; Edlund, Petra; Takala, Heikki; Ihalainen, Janne; Brayshaw, Jennifer; Norwood, Tyler; Poudyal, Ishwor; Fromme, Petra; Spence, John C. H.; Moffat, Keith; Westenhoff, Sebastian; Schmidt, Marius; Stojković, Emina A. (International Union of Crystallography, 2018)Phytochromes are red-light photoreceptors that were first characterized in plants, with homologs in photosynthetic and non-photosynthetic bacteria known as bacteriophytochromes (BphPs). Upon absorption of light, BphPs ...