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dc.contributor.advisorToppari, Jussi
dc.contributor.advisorGilbert, Leona
dc.contributor.authorShroff, Sailee
dc.date.accessioned2018-05-25T07:44:57Z
dc.date.available2018-05-25T07:44:57Z
dc.date.issued2018
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/58093
dc.description.abstractMedical devices contaminated with pathogens are the most common source of hospital acquired infections. To prevent the spreading of the infections to other patients and to ensure the safety of the medical devices, hospitals undertake obligatory decontamination procedures. The current decontamination procedures use expensive and hazardous disinfectants and lengthy sterilization protocols. Some bacteria have also developed resistant strains against common disinfectants, as well as against antibiotics of a similar structures through the process of cross resistance. Thus, there is an emerging need for the development of an alternate class of antibacterial agents that can be coated on all categories of medical devices and are active against a broad range of pathogens. Studies on silver nanoparticles in the past have highlighted their antibacterial nature, but toxicity associated with metallic silver has limited its applicability in biomedical science. With a future aim of developing an antibacterial coating for medical devices that overcomes all the above problems, we synthesized silver nanotriangles using biocompatible polymers like polyethylene glycol (PEG) and poly(sodium) styrene sulphonate (PSSS). The use of biocompatible polymers as a surface coating is hypothesized to reduce the cytotoxicity associated with the nanoparticle. Nanotriangles were of particular interest to us due to their high reactivity with bacterial surfaces that comes from their pointy vertexes and the presence of large number of high atom density facets such as {111}, which are not present in other shapes such as sphere, rods, etc. Techniques like light spectroscopy and Transmission electron microscopy were used to characterize the nanotriangles. Nanotriangles of PEG were mostly triangular with sharp edges and 35.6 % of them had edge length between 40-50 nm, while the nanotriangles of PSSS were quasi spherical to triangular with blunt edges and 37.5 % of them had edge length between 20-30 nm. The antimicrobial effect of nanotriangles of PEG and PSSS on bacteria were examined using agar plates and liquid cultures of Escherichia coli strain (DH5α). At lower dilution ratios (3/5) of PEG and PSSS nanotriangles, a clear antibacterial effect was observed, whereas, higher dilution ratios (1/10) only revealed a reduction in growth of the E. coli cells. With these set of results, silver nanotriangles made using biocompatible polymers provides innovative prospects in being used as a future antibacterial coating for medical devices.en
dc.format.extent40
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.titleExploring the antibacterial property of silver nanotriangles synthesized using biocompatible polymers
dc.identifier.urnURN:NBN:fi:jyu-201805252766
dc.type.ontasotPro gradu -tutkielmafi
dc.type.ontasotMaster’s thesisen
dc.contributor.tiedekuntaMatemaattis-luonnontieteellinen tiedekuntafi
dc.contributor.tiedekuntaFaculty of Sciencesen
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.yliopistoJyväskylän yliopistofi
dc.contributor.yliopistoUniversity of Jyväskyläen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineCell and molecular biologyen
dc.rights.copyrightJulkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.fi
dc.rights.copyrightThis publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.en
dc.type.publicationmasterThesis
dc.contributor.oppiainekoodi4013
dc.subject.ysobakteerit
dc.subject.ysokolibakteerit
dc.subject.ysoantimikrobiset yhdisteet
dc.subject.ysodesinfiointi
dc.subject.ysonanobiotekniikka
dc.subject.ysobacteria
dc.subject.ysoEscherichia coli
dc.subject.ysoantimicrobial compounds
dc.subject.ysodisinfection
dc.subject.ysonanobiotechnology
dc.format.contentfulltext
dc.type.okmG2


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